Fifteen-nuclear silver cluster taking tri((diphenylphosphine)methene)amine as ligand as well as synthetic method and antitumor application thereof

A synthesis method and cluster technology, applied in the field of medicine, can solve problems affecting ribosomal subunit protein expression, cell apoptosis, damage, etc., and achieve good medicinal value and strong anti-tumor activity

Inactive Publication Date: 2018-04-24
GUANGXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no clear conclusion about its anti-tumor mechanism. It can enter cells through different ways and generate reactive oxygen species (ROS), which can damage certain components of cells, such as DNA double strands. Destroy and affect the expression of ribosomal subunit proteins, resulting in apoptosis or death of cells
There are many studies on the effect of silver and nano-silver compounds on tumor cells, but because the stability of silver compounds has been perplexing its application as a drug, the present invention has developed a stable and as yet unreported tri((diphenyl Pentanuclear silver clusters with phosphine)methylene)amines as ligands and related reports on their synthesis methods, structures and applications

Method used

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  • Fifteen-nuclear silver cluster taking tri((diphenylphosphine)methene)amine as ligand as well as synthetic method and antitumor application thereof
  • Fifteen-nuclear silver cluster taking tri((diphenylphosphine)methene)amine as ligand as well as synthetic method and antitumor application thereof
  • Fifteen-nuclear silver cluster taking tri((diphenylphosphine)methene)amine as ligand as well as synthetic method and antitumor application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Clusters [Ag 15 (N-triphos) 4 (Cl 4 )](NO 3 ) 3 Synthesis

[0026] 1) Dissolve 60mg (0.35mmol) silver nitrate in 0.5mL distilled water, add 2.5mL methanol after complete dissolution, add 30mg (0.05mmol) NP 3 Place on a magnetic stirrer and continue stirring for 30 min. After complete reaction, add 6 mL (0.53 mmol) of newly configured sodium borohydride solution and react at room temperature for 20 h;

[0027] 2) After the reaction, centrifuge in a high-speed centrifuge at 10,000 r / min for 2 minutes, discard the supernatant reaction liquid, dissolve and extract with 7 mL of chloroform, and obtain black crystals after one week of ether vapor phase diffusion, which is the product.

[0028] The obtained black crystals were analyzed by electrospray mass spectrometry and single crystal diffraction. Specific electrospray mass spectrometry data are as follows:

[0029] (1) ESI-MS m / z :1402 [Ag 15 (N-triphos) 4 (Cl) 4 ] 3+ 、2220 {[Ag 15 (N-triphos) 4 ...

Embodiment 2

[0033] 1) Dissolve 60mg (0.35mmol) of silver nitrate in 3mL of ethanol, add 30mg (0.05mmol) of NP3 after complete dissolution, place on a magnetic stirrer and continue stirring for 30min, and add 6mL (0.53mmol) of newly prepared boron after complete reaction Sodium hydride solution, the solution turned from colorless and transparent to black rapidly, and stirred and reacted at room temperature for 20 hours;

[0034] 2) After the reaction, centrifuge in a high-speed centrifuge at 10,000r / min for 2min, discard the supernatant reaction liquid, dissolve and extract with 1mL of anhydrous methanol and 6mL of chloroform, and obtain black crystals after five days of liquid phase diffusion in ether, which is the product. The obtained black crystals were analyzed by electrospray mass spectrometry and single crystal diffraction, and determined to be the target cluster compound [Ag 15 (N-triphos) 4 (Cl 4 )](NO 3 ) 3 .

Embodiment 3

[0036] The difference from Example 2 is that after the reaction, the solvent was spin-dried with a rotary evaporator, and then 7 mL of chloroform was added for dissolution and extraction, and the product was obtained as black crystals by diethyl ether diffusion.

[0037] The obtained black crystals were analyzed by electrospray mass spectrometry and single crystal diffraction, and determined to be the target cluster compound [Ag 15 (N-triphos) 4 (Cl 4 )](NO 3 ) 3 .

[0038] In order to fully illustrate the use of the Ag15 cluster compound with tris((diphenylphosphino)methyl)amine as ligand in pharmaceuticals, an anti-tumor activity experiment was carried out.

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Abstract

The invention discloses a fifteen-nuclear silver cluster taking tri((diphenylphosphine)methene)amine as ligand as well as a synthetic method and antitumor application thereof. A chemical formula of the cluster is [[Ag15(N-triphos)4(Cl4)](NO3)3]; the cluster has a six-cap body heart cubic structure shown as a formula (I). A preparation method of the fifteen-nuclear silver cluster comprises the following steps: taking a compound shown as a formula (II) and silver nitrate, and dissolving the compound and the silver nitrate into a polar solvent; carrying out coordination reaction according to moleratio and reducing by using sodium borohydride to obtain a target product. An Ag15 cluster disclosed by the invention shows higher antitumor activity than that of a ligand NP3 and cis-platinum, has abetter potential medicinal value and can be prepared into an antitumor drug which is applied. The structures shown as the formula (I) and the formula (II) are shown in the description.

Description

technical field [0001] The invention relates to the field of medical technology, in particular to a pentanuclear silver cluster compound with tris((diphenylphosphine)methylene)amine as a ligand, its synthesis method, structure and anti-tumor application. Background technique [0002] Malignant tumors (commonly known as cancer) are one of the major diseases that endanger human health. The number of new cancer patients in the world is about 11 million every year, and the number of cancer deaths worldwide is about 7 million every year. The defense and treatment of malignant tumors have become the focus of the world's medical circles. The growth of tumors can be inhibited to varying degrees by radiotherapy, chemotherapy, and surgery. [0003] As a metal material with high biological safety and good stability, silver has attracted the attention of many researchers. In the reported literature, it is found that silver nanomaterials not only have significant antibacterial activit...

Claims

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Application Information

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IPC IPC(8): C07F1/10A61P35/00
CPCC07B2200/13C07F1/005
Inventor 王修建彭艳倪青玲马梦霞
Owner GUANGXI NORMAL UNIV
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