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Application of beta sodium aescine in preparation of medicine for treating leukemia

A technique for sodium aescin and leukemia is applied in the application field of β-escin sodium in the preparation of medicines for treating leukemia, which can solve the problem that the research on β-escin sodium has not been reported, and achieves the induction of leukemia cell apoptosis. , The dose-effect relationship is obvious, and the anti-tumor activity is strong.

Inactive Publication Date: 2005-11-02
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, there is no report on the study of β-escin sodium in inhibiting the proliferation and inducing apoptosis of leukemia cells

Method used

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  • Application of beta sodium aescine in preparation of medicine for treating leukemia
  • Application of beta sodium aescine in preparation of medicine for treating leukemia
  • Application of beta sodium aescine in preparation of medicine for treating leukemia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1: β-escin inhibits K562 cell proliferation and induces apoptosis

[0022] 1 Materials and methods

[0023] 1.1 Main reagents and instruments

[0024] β-Aescin sodium was purchased from Yantai Luye Pharmaceutical Co., Ltd., batch number 20021006, with a purity of 88.6%. The working solution of the required concentration was prepared with IMDM culture medium, and sterilized by positive pressure filtration with a filter membrane with a pore size of 0.25 μm. save. Annexin V-FITC was purchased from PharMingen, and PI was provided by Sigma. Flow cytometer, American Coulter Company, Epics-XL type.

[0025] 1.2 Cell culture

[0026] The K562 cell line was purchased from the Shanghai Cell Institute, and was kept at 37,5% CO 2 Under the condition, with volume fraction containing 10% calf serum, penicillin (1×10 5 U.L. -1 ), streptomycin (100μmol.L -1 ) of IMDM medium for subculture, and the cells used in the experiment were all in the logarithmic growth phase.

...

Embodiment 2

[0055] Example 2: β-escin sodium inhibits HL-60 cell proliferation and induces apoptosis

[0056] 1 Materials and methods

[0057] 1.1 Materials

[0058] Cells: HL-60 cell line, provided by Shanghai Cell Institute of Chinese Academy of Sciences.

[0059] Drugs: β-escin sodium was purchased from Yantai Luye Pharmaceutical Co., Ltd., batch number 20021006, with a purity of 88.6%. The working solution was made into the required concentration with the culture medium, and sterilized by positive pressure filtration with a filter membrane with a pore size of 0.25 μm. Store at ℃. Annexin V-FITC was purchased from PharMingen, and PI was provided by Sigma. Flow cytometer, American Coulter Company, Epics-XL type.

[0060] 1.2 Method

[0061] 1.2.1 Cell culture

[0062] HL-60 cells at 37 °C, 5% CO 2 Under the condition, with volume fraction containing 10% calf serum, penicillin (1×10 5 U.L. -1 ), streptomycin (100μmol.L -1 ) of IMDM medium for subculture, and the cells used in t...

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Abstract

The present invention relates to the application of beta-aescin sodium in preparing medicine for treating leukaemia. Beta-aescin sodium can inhibit K562 and HEALTH-60 cell proliferation and induce leukaemia apoptosis and thus may be used in treating leukaemia. Beta-aescin sodium has powerful extracorporeal antitumor activity, obvious dose-effect relationship and effect strengthened with time. The present invention provides foundation for screening new medicine for leukaemia.

Description

(1) Technical field [0001] The invention relates to the application of beta-escin sodium in the preparation of medicines for treating leukemia. (2) Background technology [0002] Sodium aescin is the total saponin extracted from the dried and mature fruit of the traditional Chinese medicine Suluozi. The main active ingredients are aescin A, B, C, and D. According to the definition of DAC in Germany, aescin A and B are called β- Aescin, C, D aescin is called α-escin. β-Aescinate Sodium has the functions of anti-inflammatory exudation, eliminating swelling, increasing venous tension, improving microcirculation and promoting the recovery of limb functions. This has been confirmed in foreign pharmacological research and clinical practice. It is mainly used in brain Treatment of diseases such as edema, craniocerebral trauma, cerebral infarction, Japanese encephalitis and limb swelling. [0003] Leukemia is a malignant disease of white blood cells in human blood. Its pathologic...

Claims

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Application Information

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IPC IPC(8): A61K31/704
Inventor 牛泱平
Owner ZHEJIANG UNIV OF TECH
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