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A kind of paper chip and preparation method thereof

A paper chip and paper substrate technology, applied in chemical instruments and methods, laboratory containers, fluorescence/phosphorescence, etc., can solve the problems of restricting the development and application of paper chips, complicated procedures, and high toxicity, and achieve a simple evaluation method. Easy to operate, small dosage and controllable molecular size

Inactive Publication Date: 2020-05-26
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The whole process is time-consuming, complicated, and highly toxic, which greatly limits the further development and application of paper chips in paper-based analysis platforms. There is an urgent need to obtain a simple and green method for preparing paper chips

Method used

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  • A kind of paper chip and preparation method thereof
  • A kind of paper chip and preparation method thereof
  • A kind of paper chip and preparation method thereof

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Effect test

preparation example Construction

[0038] The present invention also proposes the preparation method of above-mentioned paper chip, and it comprises the following steps:

[0039] S1, providing the detection material, injecting the detection material into the ink cartridge of the inkjet printing device;

[0040] S2, providing a paper substrate, and putting the paper substrate into an inkjet printing device;

[0041] S3, printing the detection material into the detection area of ​​the paper substrate.

[0042] Further, in a preferred embodiment of the present invention, the inkjet printing device is a piezoelectric inkjet printer, and the protein-based noble metal nanoclusters have negative charge characteristics, which can make the detection material used as ink squeeze smoothly under the action of piezoelectricity. Out of the nozzle, it is beneficial to the inkjet printing.

[0043] Further, before putting the paper substrate into the inkjet printing device, the following steps are also included:

[0044] S4...

Embodiment 1

[0053] A paper chip provided in this embodiment is prepared according to the following steps:

[0054] (1) Soak the filter paper sheet in the silk fibroin solution with a mass fraction of 5wt% for 5 minutes, and then ventilate and dry it.

[0055] (2) Mix the protein-based nanocluster solution, glucose (probe element) and PBS buffer solution in a volume ratio of 1:5:10 to obtain inkjet printing ink, which is filtered through a 0.2 μm filter and injected Inkjet print cartridges.

[0056] (3) Turn on the inkjet printer and connect it to the computer, put the filter paper pretreated by the silk fibroin solution into the printer, and click print on the interface after the dot matrix pattern is designed to obtain a fluorescent paper chip for detecting glucose oxidase.

[0057] Such as figure 1 Shown is the physical picture of the prepared fluorescent paper chip under the irradiation of ultraviolet light. There is a circular detection area on the paper chip, which emits red fluor...

Embodiment 2

[0059] A paper chip provided in this embodiment is prepared according to the following steps:

[0060] (1) Soak the filter paper piece in the wool keratin solution with a mass fraction of 10wt% for 15 minutes, and then ventilate and dry it.

[0061] (2) Proportion the protein fund nanocluster solution, xanthine and PBS buffer solution according to the volume ratio of 1:10:50 to obtain the inkjet printing ink, which is filtered through a 0.1 μm filter and injected into the inkjet printing cartridge middle.

[0062] (3) Turn on the inkjet printer and connect it to the computer, put the filter paper pretreated by the wool keratin solution into the printer, design the dot matrix pattern and click print on the interface to obtain a fluorescent paper chip for detecting xanthine oxidase . When the visible light and ultraviolet light were irradiated, the fluorescent paper chip changed from colored to colorless due to the dropwise addition of xanthine oxidase, and the results were si...

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Abstract

The invention provides a paper chip and a preparation method thereof, and relates to the technical field of biological detection. The paper chip includes a paper base material provided with at least one detection area; the detection area is fixedly provided with a detection material by an ink-jet printing method; the detection material includes protein-based precious metal nanoclusters and a detection base unit. The preparation method comprises that the detection material is injected into an ink box of an ink-jet printing device, then the detection material is printed into the detection area of the paper base material by the ink-jet printing device, and the fluorescent paper chip is obtained. The protein-based precious metal nanoclusters are used as ink, and the fluorescent paper chip is prepared by the ink-jet printing method; the paper chip can be used for detecting a variety of biomolecules, and is simple, portable and easy to manipulate; and the preparation method is simple and easy to realize.

Description

technical field [0001] The invention relates to the field of biological detection, and in particular to a paper chip and a preparation method thereof. Background technique [0002] In 2007, the Whitesides research group first proposed the concept of paper chips. Due to the abundant sources, low cost, stable chemical properties, convenient carrying capacity, and good biodegradability of paper substrates, combined with other mature technologies, devices for separation, reaction, and detection can be easily prepared . Compared with traditional microfluidic chips, the analysis components of paper chips do not require external fluid pump equipment or complex circuit design and chip design, and can achieve the purpose of sample delivery and transmission through the capillary action of cellulose fibers in paper chips. At the same time, the softness of paper chips provides a better platform and greater possibility for the innovation of subsequent processing technologies. Therefore...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01L3/00G01N21/64B41J2/01B41J3/44
CPCB01L3/5027B01L3/502707B01L2300/126B41J2/01B41J3/44G01N21/6428G01N2021/6432
Inventor 刘向阳刑瑶
Owner XIAMEN UNIV
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