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Saccharomyces cerevisiae BY21 for preventing and controlling postharvest diseases of fruits and vegetables, and preparation method and using method of saccharomyces cerevisiae BY21

A technology of Saccharomyces cerevisiae and BY21, which is applied in the field of Saccharomyces cerevisiae for biological control of postharvest diseases of fruits and vegetables, can solve the problems that the biocontrol effect is only verified on a small number of fruits, lack of strains with antibacterial spectrum, etc., and achieve significant social and ecological benefits, Stable properties and high safety effects

Active Publication Date: 2018-04-13
北京华康信使科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, although there are nearly a hundred species of antagonistic yeasts reported at home and abroad, the biocontrol effects of most antagonistic yeasts have only been verified on a few fruits.
However, since the biocontrol effects of different strains of the same yeast are very different (Filonow et al., 1996), most of the antagonistic yeasts lack strains with broad antibacterial spectrum and stable effect.

Method used

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  • Saccharomyces cerevisiae BY21 for preventing and controlling postharvest diseases of fruits and vegetables, and preparation method and using method of saccharomyces cerevisiae BY21
  • Saccharomyces cerevisiae BY21 for preventing and controlling postharvest diseases of fruits and vegetables, and preparation method and using method of saccharomyces cerevisiae BY21
  • Saccharomyces cerevisiae BY21 for preventing and controlling postharvest diseases of fruits and vegetables, and preparation method and using method of saccharomyces cerevisiae BY21

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1: Biological properties of Saccharomyces cerevisiae BY21

[0018] 1. Morphological features

[0019] (1) YPDA medium (1% yeast extract powder, 2% peptone, 2% glucose, 1.8% agar, sterilized at 121°C for 20 minutes) was cultured at 26°C for 48h, and the colonies were round and white with smooth and round edges. The cell shape is ellipsoidal.

[0020] (2) After culturing in YPDA liquid medium for 24 hours, no mold was formed, the bacterial solution was turbid, and there was precipitation. Microscopically, the yeast cells were oval and budded.

[0021] 2. Molecular biological identification

[0022] The nucleic acid sequence of the Saccharomyces cerevisiae BY21 26S rDNA D1 / D2 region was amplified by PCR with the universal forward primer NL-1 (5'-GCATATCAATAAGCGGAGGAAAAG-3') and the reverse primer NL-4 (5'-GGTCCGTGTTTCAAGACGG-3'). The sequencing results of the PCR products were entered into the website www.NCBI.nlm.nih.gov for BLAST, the homologous sequences were...

Embodiment 2

[0024] The inhibitory effect of implementation example 2 Saccharomyces cerevisiae BY21 on pear fruit blue mold

[0025] 1. Experimental protocol

[0026] Take Saccharomyces cerevisiae BY21 out of the -80°C refrigerator, activate it with YPDA medium (10g of yeast extract powder, 20g of peptone, 20g of glucose, 18g of agar, 1000ml of deionized water, natural pH, sterilized at 121°C for 30min), and pick a single colony Put it into YPD liquid medium, cultivate it under the conditions of 26°C and 200r / min for 24h, centrifuge at 4000rpm for 5min, discard the supernatant, wash the collected bacteria repeatedly with sterile water for 3 times, count on the hemocytometer and prepare a concentration of 1×10 8 cells / mL of Saccharomyces cerevisiae BY21 suspension.

[0027] Activate Penicillium expansum on a PDA medium plate, culture at 26°C for 7-14 days, scrape appropriate amount of spores, and prepare a concentration of 5×10 with sterile water. 4 cells / mL of Penicillium spore suspensi...

Embodiment 3

[0031] Implementation example 3 Saccharomyces cerevisiae BY21 is to the inhibitory effect of grape postharvest disease

[0032] 1. Experimental protocol

[0033] Take Saccharomyces cerevisiae BY21 out of the -80°C refrigerator, activate it with YPDA medium (10g of yeast extract powder, 20g of peptone, 20g of glucose, 18g of agar, 1000ml of deionized water, natural pH, sterilized at 121°C for 30min), and pick a single colony Put it into YPD liquid medium, cultivate it under the conditions of 26°C and 200r / min for 24h, centrifuge at 4000rpm for 5min, discard the supernatant, wash the collected bacteria repeatedly with sterile water for 3 times, count on the hemocytometer and prepare a concentration of 1×10 8 cells / mL of Saccharomyces cerevisiae BY21 suspension.

[0034] Botrytis porri, Aspergillus aculeatus, Alternaria eichhorniae, Fusarium redolens, Colletotrichum fructicola or Trichothecium roseum ) was activated on a PDA medium plate, cultured at 26°C for 7-14 days, scrape...

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Abstract

The invention discloses saccharomyces cerevisiae BY21, which is wide in antibacterial spectrum and stable in effects, for preventing and controlling postharvest diseases of fruits and vegetables, anda using method of the saccharomyces cerevisiae BY21. The strain is preserved in China General Microbiological Culture Collection Center (CGMCC), and has a preservation number of CGMCC No. 14904. The using method of the saccharomyces cerevisiae comprises the following steps: activating a strain, carrying out fermentation culture on the strain with YPD, centrifuging, and preparing thallus into 1*10<8> cells / mL bacterial suspension with sterile water; putting fruits and vegetables such as pears, grapes, strawberries, citruses or cherry tomatoes into the bacterial suspension, soaking for 30s and then taking out, and drying in the air; putting the dried products into preservation boxes, and storing at room temperature. The saccharomyces cerevisiae strain can be used for controlling pear fruit penicillium, grape gray mold, aspergillosis, black spot, fusarium mildew, anthracnose and cephalothecium roseum, strawberry gray mold and aspergillosis, citrus penicillium as well as cherry tomato graymold and aspergillosis at the same time, and reduces the loss caused by the postharvest diseases, thus having a very good application prospect.

Description

technical field [0001] The invention relates to the field of biological control of post-harvest diseases of fruits and vegetables, in particular to a strain of Saccharomyces cerevisiae used for the biological control of post-harvest diseases of fruits and vegetables. Postharvest diseases all have significant control effects. Background technique [0002] Although the quality deterioration of fresh fruits and vegetables is affected by many factors, disease is the main reason. Among them, rot and deterioration caused by fungal diseases is the most serious factor in postharvest fruit loss. Although postharvest diseases of fruits and vegetables can be prevented and controlled in many ways such as agricultural control, physical control, chemical control and biological control, the main measure at present is chemical control (Eckert & Ogawa, 1985, 1988). However, long-term use of chemical pesticides not only leads to the development of drug resistance of pathogenic bacteria but ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/18A23B7/155C12R1/865
CPCA23B7/155C12N1/185C12R2001/865
Inventor 王友升姚婷黄津津李丽萍任向峰范雅为
Owner 北京华康信使科技有限公司
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