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Method for one-time quickly separating active polysaccharide peptide and triterpene acid of lucid ganoderma

A technology of Ganoderma lucidum triterpenoid acid and active polysaccharide, which is applied in the directions of medical preparations containing active ingredients, pharmaceutical formulations, plant/algae/fungus/moss ingredients, etc., to achieve the effects of high yield and simple process

Inactive Publication Date: 2018-04-13
福建农大菌草技术开发公司
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  • Abstract
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Problems solved by technology

[0004] The present invention will solve the problem of extraction and separation of the extraction components of the complex active components of Ganoderma lucidum

Method used

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  • Method for one-time quickly separating active polysaccharide peptide and triterpene acid of lucid ganoderma
  • Method for one-time quickly separating active polysaccharide peptide and triterpene acid of lucid ganoderma
  • Method for one-time quickly separating active polysaccharide peptide and triterpene acid of lucid ganoderma

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Embodiment 1

[0019] (1) Slice Ganoderma lucidum (thickness 2-3mm) or broken filaments, add 12-20 times the weight of deionized water, soak at room temperature for 2-4 hours, then heat at 90-100°C for 1.5-2 hours, filter, Extract the filter residue with 10 to 15 times the water at the same temperature for 1 to 1.5 hours, filter, combine the second filtrate, and centrifugally filter (3000 to 4500r / min). -0.09~-0.097MPa, the concentration specific gravity is 1.10, and the Ganoderma lucidum extract preparation solution is obtained. Take the ganoderma lucidum extract preparation liquid and pass it through the 10μm~0.2μm microporous filter membrane (mixed cellulose grease) under the pressure condition of the disc filter PALL type (ф150mm) and the peristaltic pump (Masterflex L / S type). The liquid is the microfiltrate extracted from Ganoderma lucidum, and the extraction yield is 14.74%. (After the Ganoderma lucidum extract is purified, the molecular weight of each component of the Ganoderma luci...

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Abstract

The invention discloses a method for one-time quickly separating active polysaccharide peptide and triterpene acid of lucid ganoderma. The method comprises the steps of slicing or smashing the lucid ganoderma, extracting through water, centrifuging to remove impurities, purifying, selecting three different membranes, i.e., a 10K membrane, a 6KDa membrane and a 1KDa according to a molecular weightdifference of different substances, classifying and purifying different components in an extraction solution through pore diameters from large to small, and obtaining lucid ganoderma polysaccharide peptide and ludic ganoderma triterpene acid used for developing different products or comparison products. According to the method provided by the invention, the molecular weight of different ludic ganoderma compounds is directly intercepted, so that the lucid ganoderma active polysaccharide peptide and a ludic ganoderma triterpene acid compound can be quickly separated at one time, and compared with a traditional technology, the method is simple and convenient in process, quick, high in yield, free of pollution, and suitable for industrial production.

Description

technical field [0001] The invention relates to a preparation method for one-time rapid separation of ganoderma active polysaccharide peptides and triterpenoids, in particular to a preparation method for obtaining main active components in a one-time separation of ganoderma lucidum extract by membrane separation molecular weight technology. technical background [0002] Ganoderma lucidum is a treasure in the treasure house of traditional Chinese medicine. According to the Pharmacopoeia of the People's Republic of China (Part One) (2000, 2005, 2010 editions), Ganoderma lucidum (red lucidum, purple lucidum) is recorded as a traditional Chinese medicine. Ganoderma lucidum belongs to Basidiomycetes, Polyporaceae, and fungi of the genus Ganoderma (the general name of red lucidum, purple lucidum, etc.). It has a medicinal history of more than 2,000 years in my country. "Compendium" all prove that Ganoderma lucidum has the effect of preventing and curing diseases and prolonging life...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K36/074
CPCA61K36/074C08B37/0003C08B37/006
Inventor 林树钱林冬梅王赛贞林占熺罗虹建王联福郭嘉铭
Owner 福建农大菌草技术开发公司
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