A kind of microcapsule for preventing and treating plant diseases and its preparation method and application

A technology for microcapsules and plant diseases, applied in the fields of botanical equipment and methods, chemicals for biological control, plant growth regulators, etc. The effect of anti-stress ability and good control effect

Active Publication Date: 2021-10-12
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current dosage form of Pseudomonas fluorescens is mainly wettable powder, and there is no report related to the microcapsule preparation of Pseudomonas fluorescens

Method used

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  • A kind of microcapsule for preventing and treating plant diseases and its preparation method and application
  • A kind of microcapsule for preventing and treating plant diseases and its preparation method and application
  • A kind of microcapsule for preventing and treating plant diseases and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1～5

[0034] Example 1 ~ 5 Preparation of Fluorescent Pseudomonas Microcapsules

[0035] 1. Fluorescence pseudoma fermentation fluid by liquid fermentation

[0036] 1.1 Optimization of fermentation medium

[0037] Cultivate raw materials and culture conditions

[0038] The strain used: Fluorescent Pseudomonas SN15-2 (separated from the root soil of plant soil can be purchased from the National Key Laboratory of Bioreactor Engineering of East China University).

[0039] Initial fermentation medium: 1.0% glycerol, 2.0% protein 胨, 0.15% of seven water sulfate, 2 potassium hydrogen phosphate, pH 7.0.

[0040] Replace carbon source: glucose, soluble starch, maltose, sucrose, corn flour, brown sugar, lactose, bran, glutinous rice flour, sorghum powder, wheat flour.

[0041] Replace the nitrogen source: yeast cream, yeast powder, peanut powder, bean cake powder, soy flour, tropsel, urea, ammonium sulfate, potassium nitrate.

[0042] Replace the inorganic salt: a hydrobiomide, calcium chloride,...

Embodiment 6

[0069] Example 6 Fluorescent Pseudomonas Microcapsules Field Wilt Disease Prevention Test

[0070] (1) Test method

[0071] In the field soil in the field soil, the three districts, the three districts, respectively, the fluorescent pseudocellular bacilli microcapsules prepared by Examples 2, 4, and 5, respectively, and the five groups of parallel tests were divided. From:

[0072] Control group 1 (blank control): Do not use any medication;

[0073] Control group 2 (acouma ester): treated with pyrostera;

[0074] Experimental Group 1: Using 100 million CFU / G Fluorescent Pseudocensis Microcapsules 300 times liquid;

[0075] Experimental Group 2: Use 500 million CFU / G fluorescent fake single cell bacilli microcapsules 300 times liquid;

[0076] Experimental Group 3: Treatment of 300 times using 1 billion CFU / G fluorescent fake single cell bacilli microcapsules;

[0077] The above-mentioned group treatment zone is applied to 500 ml of a pot of 500 ml per plant, and the second a...

Embodiment 5

[0089] Table 3 Example 5 Fluorescent Pseudomonasyl Microcapsules for Wilt Sickness of Wilt Wilt

[0090]

[0091] Table 4 Example 4 Fluorescent Pseudocentenelli microcapsules to prevent watermelon

[0092]

[0093] Table 5 Example 2 Fluorescent Pseudocentene Micropap Capsule for Wilt Wilt Sickness

[0094]

[0095] The experimental results show that the fluorescent pseudocellular bacilli microcapsules used in Examples 2, 4, 5 of the present invention 20 days after watermelon, and the rawlings of watermelon is reduced before the application, and three groups were implemented. Each treatment in the example is significantly lower than the blank control. In Example 5 1 billion CFU / G fluorescent fake single cell bacilli microcapsules treatment was highest, reaching 69.56%, comparable to chemical pesticide, lower concentration of fluorescent fake single cell bacilli microcapsules Watermelon has also shown a better prevention and treatment effect. After 2 administrations, the har...

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Abstract

The invention discloses a microcapsule for preventing and treating plant diseases. The microcapsule comprises Pseudomonas fluorescens, a protective agent and a carrier; the protective agent is selected from maltodextrin, sodium caseinate and carboxymethyl One or more than two kinds of base cellulose sodium. The invention also discloses the preparation method and application of the microcapsule. The invention adopts liquid fermentation to produce Pseudomonas fluorescens as an active ingredient, and organic compounding of carrier, protective agent and Pseudomonas fluorescens forms Pseudomonas fluorescens microcapsule preparation. The microcapsules of the present invention can be used to prevent and treat a variety of plant soil-borne diseases, especially have better control effects on vegetable bacterial wilt and plant wilt, and have stronger vitality than live bacteria preparations produced by traditional methods And stress resistance, more functions, more suitable for production applications. It solves the current Pseudomonas fluorescens biopesticide's short shelf life and high mortality rate in the processing process, which cannot be mass-produced and the dust problems in the production and application process.

Description

Technical field [0001] The present invention belongs to the field of plant disease biological prevention and treatment, and is specifically related to microcapsules for preventing plant diseases and their preparation methods and applications. Background technique [0002] Green blight is a world-defined earth biopathy caused by Ralstonia Solanacearum, referred to as cyprotypes. It is easy to get in high temperature and high humidity. It is widely distributed in tropics. The subtropical and temperate regions, even in some low temperature areas, the existence of cypros, giving great threats to global crop production. There are several chemical pesticides and bio-pesticides that prevent cypropolism, and combined with traditional governance methods, it plays a certain effect on the prevention and treatment of green blight, but it is still not very fluent. Great progress. At present, chemical prevention and treatment of green blight mainly use chemical pesticides such as caminecuro, F...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N25/28A01N63/27A01P1/00A01P3/00
CPCA01N25/28A01N63/10
Inventor 王伟王晓冰陈俊
Owner EAST CHINA UNIV OF SCI & TECH
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