Split bioreactor

Abstract

The invention relates to a split bioreactor. The split bioreactor is composed of a pressure bin, culture bins and pressure seal devices; the several culture bins with independent seal structures can be placed in the pressure bin, and the pressure bin use efficiency can be improved by replacing the culture bins; a pressure gas inlet pipe and a pressure gas outlet pipe are installed in a pressure bin box, the contact parts of a pressure bin cover and the pressure bin box are in sealed connection through a seal gasket, and the pressure bin cover is fixed to the pressure bin box through fast connectors; the pressure seal devices corresponding to the culture bins are installed on the pressure bin cover separately, annular mechanical grippers are connected with guide holes in the pressure bin cover through transmission rods on the annular mechanical grippers, and the transmission rods are in sealed connection with the guide holes in the pressure bin cover through seal elements. When the interior of the pressure bin is pressurized, the annular mechanical grippers press gel silica membranes of the culture bins under driving of the pressure seal devices, so that sealing is formed between the gel silica membranes and the upper end faces of culture tanks, liquid will not overflow when the pressure bin is pressurized, and safe sterile operation is achieved.

Description

technical field [0001] The invention relates to a bioreactor, in particular to a split type bioreactor, and belongs to the technical field of bioreactors. Background technique [0002] A bioreactor is an experimental tool used to cultivate biological cells and tissues. It provides cells and tissues with a high-pressure culture environment for 1 hour a day. One blow, aseptic (this is the basis of cell culture), sufficient nutritional environment (including the supply of nutrients (the degradation rate of various cells must be fast) and the removal of waste), to meet the needs of biological tissues every other day liquid. [0003] Integrated bioreactor with existing circulation system and pressurization system. Such as patent publication number CN103589638B, a pneumatic self-circulation animal cell culture bioreactor and its use method, the magnetic stirring system, heating system and control system located at the bottom of the large tank, the upper end of the large tank is ...

AI Technical Summary

Problems solved by technology

The operation is complex, prone to human error, resulting in liquid leakage, once the liquid leaks, it will cause pollution

The integrated design does not allow routine liquid changes every other day, and cannot meet the nutritional conditions of the cell and tissue culture environment

The integrated design results in a bulky pressurized chamber that carries cultured tissues in the incubator for long-term cultivation. The humid and hot environment in the incubator causes rapid aging of components, reduces the stability of the equipment, and increases the risk of contamination

The integrated design makes the operation of putting cells and tissues into the reactor and taking them out of the reactor complicated, and it is an operation with a high risk of contamination

Integrated design, in order to achieve the safety conditions of the internal pressurized chamber in a high-pressure environment, the reactor is designed to be heavy, and it is difficult to carry and operate for practical operation

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