Cultivation method for new variety of Chinese herbaceous peony
A breeding method and technology of new varieties, applied in the field of cultivating new varieties of peony, can solve the problems of affecting the mutation rate and mutagenesis survival rate, low probability of new varieties of peony, and difficulty in producing flower and color varieties, so as to improve seed cell activity and germination efficiency, increase seed cell activity, improve nutrient absorption and resistance
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Embodiment 1
[0016] A method for cultivating new varieties of peony, comprising the following steps:
[0017] (1) Storing at constant temperature: first sterilize and dip the peony seeds, and then put them in an incubator for storage at a temperature of 45°C and a humidity of 81% for 4.5 hours. Prepare preserved seeds;
[0018] (2) Ionization activation: Immerse the preserved seeds in the inducer for ionization. The colchicine, aloperine and galantamine contained in the inducer can increase the mutation probability of the seeds. Treat them under the conditions of voltage 47V and current 0.64A 38min, the alternating frequency of the positive and negative poles is 6 times / min, the seeds are stimulated by the alternating electric current, the activity of the seed cells is improved, and the mutagenesis rate of the highly active seeds will be higher, and the ionized seeds will be obtained;
[0019] (3) Variable temperature mutagenesis: immerse the ionized seeds in the protective solution and p...
Embodiment 2
[0026] (1) Storing at constant temperature: first sterilize and dip the peony seeds, and then put them in an incubator for storage at a temperature of 47°C and a humidity of 84%, and store them for 5.5 hours, which can make the seeds quickly absorb water and expand, and improve the activity and germination rate of seed cells. Prepare preserved seeds;
[0027] (2) Ionization activation: Immerse the preserved seeds in the inducer for ionization. The colchicine, aloperine and galantamine contained in the inducer can increase the mutation probability of the seeds, and treat them under the conditions of voltage 49V and current 0.72A 39 minutes, the alternating frequency of positive and negative poles is 6 times / min, and the seeds are stimulated by constantly changing the electric current to improve the activity of seed cells, and the mutagenesis rate of highly active seeds will be higher, and ionized seeds will be obtained;
[0028] (3) Variable temperature mutagenesis: immerse the...
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