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Preparation of liquid collagen submucosal filler

A filler, collagen technology, applied in the field of biomaterials, can solve problems such as tissue damage, and achieve the effect of reducing bleeding and perforation

Inactive Publication Date: 2018-02-13
BEIJING HOTWIRE MEDICAL TECH DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Other injections such as hypertonic saline will cause different degrees of tissue damage

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Take 0.01mol / L disodium hydrogen phosphate solution, add MES solution with pH=6 to it, until the pH of the mixed solution reaches 7, dilute the neutralized mixed solution 20 times with deionized water to make a neutral buffer solution .

[0050] Take 20g of type I collagen with a purity of more than 99% and a natural triple helix structure (hydroxyproline content > 11%), put it in 600ml of neutral buffer, stir for 5-10min until uniform, and let it soak for 1.5h.

[0051] Add 1.25ml of 38.5% formaldehyde solution to the neutral buffer solution, stir for 7 minutes to make the mixture uniform, and then place it in a constant temperature water bath at 20°C at a speed of 200 rpm for 3 hours to react and crosslink.

[0052] After the reaction, the reaction product was taken out, washed with buffer solution for 30 minutes, and then washed with deionized water for 1 hour.

[0053] Freeze-dry the cleaned collagen, first pre-freeze at -40°C for 1 hour, then pre-freeze at -20°C f...

Embodiment 2

[0057] Take 0.01mol / L disodium hydrogen phosphate solution, add MES solution with pH=6 to it, until the pH of the mixed solution reaches 7, dilute the neutralized mixed solution 20 times with deionized water to make a neutral buffer solution .

[0058] Take 20g of type I collagen with a natural triple helix structure with a purity of more than 99%, put it in 600ml of buffer solution, stir for 5-10min until uniform, and let it stand for 1.5h.

[0059] Add 1.56ml of 38.5% formaldehyde solution to the buffer solution, stir for 10 minutes to make the mixture uniform, and then place it in a constant temperature water bath at 40°C at a speed of 220 rpm for 3.5 hours to react and crosslink.

[0060] After the end, the collagen was taken out, washed with buffer solution for 40 minutes, and then washed with deionized water for 1.5 hours.

[0061] Freeze-dry the collagen, first pre-freeze at -40°C for 1 hour, then pre-freeze at -20°C for 3 hours, and keep the temperature of the freezer...

Embodiment 3

[0065] Weigh 4g of the collagen powder obtained in Example 1 or Example 2, add it to 100ml of phosphate buffer solution with a concentration of 0.5mmol / L and pH=7.5, and stir evenly.

[0066] Then the mixed solution is subjected to low-temperature and high-pressure homogenization, the homogenization setting temperature is 0° C., and the homogenization pressure is 800 bar. Repeat the homogenization 9 times. A liquid collagen submucosal filler with a collagen particle size of 50-150 μm and a collagen concentration of 40 mg / ml was obtained.

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Abstract

The invention discloses preparation of a liquid collagen submucosal filler. Collagen is of type I and has a complete natural triple helix structure, and a hydroxyproline content is greater than 11%. The type I collagen and type I collagen powder prepared from the type I collagen are particularly applicable to preparation of the liquid collagen submucosal filler; and the obtained liquid collagen submucosal filler can serve as a rising agent of a focus in endoscopic mucosal resection or endoscopic submucosal decollement and a repair material of a wound in an operation process, is reasonable in support time span, can be absorbed by a tissue completely, has hemostasis and repair functions at the same time, and has a very good application effect.

Description

technical field [0001] The invention relates to the field of biomaterials, in particular to type I collagen and a liquid collagen submucosal filler prepared therefrom. Background technique [0002] At present, with the popularity of endoscopy, the detection rate of precancerous lesions and early cancers is gradually increasing. Endoscopic submucosal dissection (ESD) or endoscopic mucosal resection (EMR) has gradually become a standard minimally invasive treatment for resection of precancerous lesions and early cancers of the digestive tract, which can completely resect the lesions en bloc , thereby greatly reducing the risk of tumor recurrence. [0003] ESD surgery also has greater risks, and some complications may occur, mainly including gastrointestinal perforation and bleeding. In view of the phenomenon of perforation in patients, in order to make it safer and more convenient, most scholars at home and abroad have confirmed that submucosal injection before surgical rese...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/78A61L27/24A61L27/58A61L31/04A61L31/14
CPCA61L27/24A61L27/58A61L31/044A61L31/148A61L2300/252A61L2300/412A61L2300/604A61L2400/06C07K14/78
Inventor 张慧敏富勇
Owner BEIJING HOTWIRE MEDICAL TECH DEV CO LTD
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