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Detection kit for acute kidney injury

A detection kit and technology for acute kidney injury, applied in the field of detection kits for acute kidney injury, can solve the problems of poor repeatability, complex reaction kinetics, inappropriateness and the like

Inactive Publication Date: 2018-02-09
FAPON BIOTECH INC +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

ELISA has low sensitivity and is mainly qualitative detection
Among them, horseradish peroxidase (HRP), a luminescent marker commonly used in chemiluminescent enzyme immunoassays, can be used for quantitative detection of some proteins, but the substrate luminescence of HRP has complex reaction kinetics and poor repeatability, so it is not suitable for detection of concentration. Relatively small fluctuation range protein detection
[0007] In summary, the traditional acute kidney injury detection kit based on NGAL as a diagnostic marker has low sensitivity and poor specificity

Method used

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  • Detection kit for acute kidney injury

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Experimental program
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Effect test

Embodiment 1

[0069] The acute kidney injury detection kit includes a first detection solution, a second detection solution, a substrate solution and NGAL standard products. Wherein the first detection solution contains the first anti-NGAL monoclonal antibody coated with magnetic particles at a concentration of 0.5 mg / mL (the mass ratio of magnetic particles to the first anti-NGAL monoclonal antibody is 10 μg:1 mg), and the concentration is 50 mmol / L HEPES (4-hydroxyethylpiperazineethanesulfonic acid), 1% BSA by mass fraction, 1% sodium chloride (NaCl) by mass fraction, 5% sucrose by mass fraction, 0.1% by mass fraction Tween-20 and PC-300 with a mass fraction of 0.1%, the rest of ultrapure water, and the pH value of the first detection solution is 7.0. The second detection solution contains the second anti-NGAL monoclonal antibody labeled with alkaline phosphatase at a concentration of 1 μg / mL (the molar ratio of alkaline phosphatase to the second anti-NGAL monoclonal antibody is 1:1), and...

Embodiment 2

[0116] The acute kidney injury detection kit includes a first detection solution, a second detection solution and NGAL standard products. Wherein the first detection solution contains the first anti-NGAL monoclonal antibody coated with magnetic particles at a concentration of 0.5 mg / mL (the mass ratio of magnetic particles to the first anti-NGAL monoclonal antibody is 30 μg:1 mg), and the concentration is 60 mmol / L HEPES (4-hydroxyethylpiperazineethanesulfonic acid), 2% BSA by mass fraction, 2% sodium chloride (NaCl) by mass fraction, 10% sucrose by mass fraction, 1% by mass fraction Tween-20 and PC-300 with a mass fraction of 0.1%, the rest of ultrapure water, and the pH value of the first detection solution is 7.0. The second detection solution contains the second anti-NGAL monoclonal antibody labeled with alkaline phosphatase at a concentration of 1 μg / mL (the molar ratio of alkaline phosphatase to the second anti-NGAL monoclonal antibody is 1:4), and the concentration is 1...

Embodiment 3

[0118]The acute kidney injury detection kit includes a first detection solution and a second detection solution. Wherein the first detection solution contains the first anti-NGAL monoclonal antibody coated with magnetic particles at a concentration of 0.5 mg / mL (the mass ratio of magnetic particles to the first anti-NGAL monoclonal antibody is 5 μg:1 mg), and the concentration is 40 mmol / L HEPES (4-hydroxyethylpiperazineethanesulfonic acid), 10% BSA by mass fraction, 2% sodium chloride (NaCl) by mass fraction, 10% sucrose by mass fraction, 1% by mass fraction Tween-20 and PC-300 with a mass fraction of 0.1%, the rest of ultrapure water, and the pH value of the first detection solution is 7.0. The second detection solution contains the second anti-NGAL monoclonal antibody labeled with alkaline phosphatase at a concentration of 1 μg / mL (the molar ratio of alkaline phosphatase to the second anti-NGAL monoclonal antibody is 1:0.5), and the concentration is 100 mmol / L of Tris-HCl...

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Abstract

The invention relates to a detection kit for acute kidney injury. The detection kit comprises a first detection solution and a second detection solution, wherein the first detection solution containsa first anti-NGAL monoclonal antibody coated with magnetic particles, and the second detection solution contains an alkaline phosphatase-labeled second anti-NGAL monoclonal antibody. The first anti-NGAL monoclonal antibody and the second anti-NGAL monoclonal antibody are against different NGAL epitopes, respectively. The detection kit for acute kidney injury carries out detection with NGAL (neutrophil gelatinase-associated lipocalin) as a diagnosis and detection marker, can rapidly detect and diagnose acute kidney injury in an early stage of acute kidney injury, and has high sensitivity and specificity during detection.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a detection kit for acute kidney injury. Background technique [0002] Acute kidney injury (acute kidney injury, AKI) is a clinical syndrome caused by a sudden decline in renal function in a short period of time (hours to days) caused by various reasons, and is a common disease that threatens the lives of critically ill patients. Usually, more than 50% of patients in intensive care have different degrees of kidney damage, and the mortality rate of acute renal failure is at the forefront of the mortality rate of critical illness. In the past few decades, people's understanding of the pathogenesis of AKI and the level of medical technology have been greatly improved, but the morbidity and mortality of AKI are still high. One of the main reasons leading to this situation is the lack of effective early diagnostic markers. Traditional diagnostic indicators (serum creatinine sCr, ...

Claims

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Application Information

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IPC IPC(8): G01N33/535G01N33/543G01N33/577
CPCG01N33/535G01N33/5434G01N33/577
Inventor 李瑞净赵存洋游梅香池朗山丁正辉胡金灵
Owner FAPON BIOTECH INC
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