A detection method of form mercury in animal tissue cells
A detection method and animal tissue technology, applied in measurement devices, instruments, scientific instruments, etc., can solve the problems of high detection limit, poor selectivity, unfavorable trace form mercury detection, etc., and achieve stability enhancement and mutual conversion prevention. Effect
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Embodiment 1
[0024] The detection method of form mercury in animal tissue cells of the present invention comprises the following steps:
[0025] (1) Sample pretreatment: After treating the cell sample with 0.3% triton for 15 minutes, sonicate for 60 minutes, shake and shake, take 100 μL of sample solution, add 500 μL of extractant, shake and shake well, centrifuge at 12,000 rpm for 15 minutes at 4°C, and take the supernatant The liquid was analyzed by UPLC-ICP-MS after passing through a 0.22μm filter membrane;
[0026] (2) Chromatographic conditions: Chromatographic column: Althena C18, 120A, 4.6×250mm, 5μm; mobile phase: 10mmol / L ammonium acetate, 0.05% L-cysteine, 5% methanol, adjust pH to 2.0- with concentrated hydrochloric acid 2.5, dilute to 1L with primary water; flow rate: 1.1mL / min; injection volume: 20μL;
[0027] (3) Mass spectrometry conditions: ICP-MS working parameters: optimize the instrument conditions with 1.0μg / L tuning solution before measurement, among them, ICP-MS of T...
Embodiment 2
[0030]Standard music: Inorganic mercury, methylmercury, and ethylmercury standard solutions were purchased from the China Institute of Metrology, diluted step by step to 1000μg / L and then mixed to prepare a mixed standard solution containing 100μg / L of mercury, and then extracted solution and add a certain amount of cell culture medium to dilute inorganic mercury, methylmercury and ethylmercury step by step. Supernatant detection; on-machine detection obtained r2=0.9998 for inorganic mercury, f(x)=16373.8678*x+161.7391; r2=0.9998 for methylmercury, f(x)=14744.4700*x+214.245; r2=0.9997f(x)=14601.0805*x+69.5239; linear range: 0~5μg / L.
Embodiment 3
[0032] Recovery rate: After adding a certain amount of three forms of mercury mixture to the cultured cells, extract and detect the data of group A; then culture 4 copies of the same tissue cells under the same conditions as the data of group A, and select two of them to directly extract the form Mercury, get the data of group B, add three kinds of mercury mixed solution according to the ratio of 1:1 of the data of group A, and extract the inorganic mercury under the same conditions after shaking well, get the data of group C, get the recovery rate after calculation, the mercury is 91.6 %; methylmercury is 96.7%, ethylmercury is 97.8%.
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