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Process method for concentrating soybean peptide by tandem membranes

A process method and soybean peptide technology, applied in the field of using tandem membrane to concentrate soybean peptides, can solve the problems of microbial bad smell, product quality influence, long production cycle, etc., and achieve the effect of shortening production cycle, improving efficiency and short production cycle.

Active Publication Date: 2018-02-09
NUOLI RUYI ANYANG BIOLOGICAL SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The main problems of soybean peptide products on the market today are the large molecular weight and heavy bitter and salty taste.
[0004] At present, soybean peptide manufacturers at home and abroad use evaporation concentration or single-membrane double-membrane concentration soybean peptide liquid. Such methods generally require a lot of energy or a long time to concentrate soybean peptide liquid to spray concentration, resulting in long retention time of peptide liquid and microorganisms. It is easy to reproduce and produce some bad smells. At the same time, due to the long production cycle found in the production process, it will affect the quality of the product in many aspects

Method used

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  • Process method for concentrating soybean peptide by tandem membranes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A process method for concentrating soybean peptides using serial membranes, the steps are as follows:

[0020] (1) Using commercially available soybean protein isolate as raw material, add saturated NaOH solution at 20°C, adjust the pH to 9, and perform alkali dissolution on the soybean protein isolate to obtain a soybean protein isolate alkaline solution;

[0021] (2) Keep the temperature of the above soybean protein isolate alkaline solution at 40°C, add Alcalase protease according to 10‰ of the weight of soybean protein isolate, stir for 2 hours, and when the degree of hydrolysis DH=20%, adjust the pH to 4.0 to inactivate the enzyme, 3500r / min centrifuged for 15min, the obtained supernatant is the crude soybean peptide liquid, the sugar content of the crude soybean peptide liquid is 20BX, and the pH value is 6;

[0022] (3) Perform total reflux nanofiltration operation on the crude soybean peptide liquid at room temperature, and perform adsorption and deodorization ...

Embodiment 2

[0026] A process method for concentrating soybean peptides using serial membranes, the steps are as follows:

[0027] (1) Using commercially available soybean protein isolate as raw material, add a saturated NaOH solution at 45°C, adjust the pH to 5, and perform alkali dissolution on the soybean protein isolate to obtain a soybean protein isolate alkaline solution;

[0028] (2) Keep the temperature of the above soybean protein isolate alkali solution at 70°C, add Alcalase protease according to 1‰ of the weight of soybean protein isolate, stir for 4 hours, and when the degree of hydrolysis DH=20%, adjust the pH to 4.0 to inactivate the enzyme, 3000r / min centrifugation for 20min, the obtained supernatant is the crude soybean peptide liquid, the sugar content of the crude soybean peptide liquid is 5BX, and the pH value is 11;

[0029] (3) Perform total reflux nanofiltration operation on the crude soybean peptide liquid at room temperature, and perform adsorption and deodorizatio...

Embodiment 3

[0033] A process method for concentrating soybean peptides using serial membranes, the steps are as follows:

[0034] (1) Using commercially available soybean protein isolate as raw material, add saturated NaOH solution at 25°C, adjust the pH to 8, and perform alkali dissolution on the soybean protein isolate to obtain a soybean protein isolate alkaline solution;

[0035] (2) Keep the temperature of the above soybean protein isolate alkaline solution at 50°C, add Alcalase protease according to 5‰ of the weight of soybean protein isolate, stir for 3 hours, and when the degree of hydrolysis DH=20%, adjust the pH to 4.0 to inactivate the enzyme, 3200r / min centrifugation for 18min, the obtained supernatant is the crude soybean peptide liquid, the sugar content of the crude soybean peptide liquid is 10BX, and the pH value is 8;

[0036] (3) Perform total reflux nanofiltration operation on the crude soybean peptide liquid at room temperature, and perform adsorption and deodorizatio...

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Abstract

The invention discloses a process method for concentrating soybean peptide by tandem membranes. According to the method, firstly, a soybean protein isolate alkali solution is prepared; Alcalase protease is added into the soybean protein isolate alkali solution; enzyme deactivation is performed after enzymolysis; a liquid supernatant obtained after centrifugation is a crude soybean peptide solution; the crude soybean peptide solution is subjected to total reflux nanofiltration operation at room temperature; during the nanofiltration operation, active carbon adsorption and deodorization are performed; six nanofiltration membranes are used for tandem during the nanofiltration operation; the interception molecular weight is 150 Da to 2000 Da; the pressure is 0.2MPa to 1.0MPa; after the treatment is performed for 5 to 20min, and inorganic salt ions with the relative molecular mass being less than or equal to 150 Da are removed, a filtered soybean peptide mixed solution is collected; and spray drying is performed, and the soybean peptide is prepared. The six nanofiltration membranes are used for tandem treatment operation; the dry substance content of the materials is improved; the costis reduced; the production period is shortened; meanwhile, the active carbon adsorption deodorization and debitterization is performed, and the foreign taste of the soybean peptide can be thoroughly eliminated; and the clear and transparent state of the soybean peptide mixed solution is maintained.

Description

technical field [0001] The invention belongs to the technical field of soybean protein deep processing, and in particular relates to a process method for concentrating soybean peptides by adopting serial membranes. Background technique [0002] Soybean peptide, also known as soybean peptide, is an enzymatic hydrolysis product of soybean protein, usually a peptide mixture composed of 3-6 amino acids, and its molecular weight is mainly concentrated below 1000. As an excellent health food, it not only has the effects of lowering blood fat, easy digestion, easy absorption, promoting energy metabolism and anti-obesity, but also can eliminate fatigue, enhance muscle stretching and anti-allergy. The product integrates nutritional characteristics, health characteristics and processing performance, has multiple regulating functions on the body, and is widely used in the production of food and health products. [0003] The main problems of soybean peptide products on the market today...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/34
CPCC07K1/34C12P21/06
Inventor 刘新旗付慧彦张海萍王华
Owner NUOLI RUYI ANYANG BIOLOGICAL SCI & TECH
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