Process method for concentrating soybean peptide by tandem membranes
A process method and soybean peptide technology, applied in the field of using tandem membrane to concentrate soybean peptides, can solve the problems of microbial bad smell, product quality influence, long production cycle, etc., and achieve the effect of shortening production cycle, improving efficiency and short production cycle.
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Embodiment 1
[0019] A process method for concentrating soybean peptides using serial membranes, the steps are as follows:
[0020] (1) Using commercially available soybean protein isolate as raw material, add saturated NaOH solution at 20°C, adjust the pH to 9, and perform alkali dissolution on the soybean protein isolate to obtain a soybean protein isolate alkaline solution;
[0021] (2) Keep the temperature of the above soybean protein isolate alkaline solution at 40°C, add Alcalase protease according to 10‰ of the weight of soybean protein isolate, stir for 2 hours, and when the degree of hydrolysis DH=20%, adjust the pH to 4.0 to inactivate the enzyme, 3500r / min centrifuged for 15min, the obtained supernatant is the crude soybean peptide liquid, the sugar content of the crude soybean peptide liquid is 20BX, and the pH value is 6;
[0022] (3) Perform total reflux nanofiltration operation on the crude soybean peptide liquid at room temperature, and perform adsorption and deodorization ...
Embodiment 2
[0026] A process method for concentrating soybean peptides using serial membranes, the steps are as follows:
[0027] (1) Using commercially available soybean protein isolate as raw material, add a saturated NaOH solution at 45°C, adjust the pH to 5, and perform alkali dissolution on the soybean protein isolate to obtain a soybean protein isolate alkaline solution;
[0028] (2) Keep the temperature of the above soybean protein isolate alkali solution at 70°C, add Alcalase protease according to 1‰ of the weight of soybean protein isolate, stir for 4 hours, and when the degree of hydrolysis DH=20%, adjust the pH to 4.0 to inactivate the enzyme, 3000r / min centrifugation for 20min, the obtained supernatant is the crude soybean peptide liquid, the sugar content of the crude soybean peptide liquid is 5BX, and the pH value is 11;
[0029] (3) Perform total reflux nanofiltration operation on the crude soybean peptide liquid at room temperature, and perform adsorption and deodorizatio...
Embodiment 3
[0033] A process method for concentrating soybean peptides using serial membranes, the steps are as follows:
[0034] (1) Using commercially available soybean protein isolate as raw material, add saturated NaOH solution at 25°C, adjust the pH to 8, and perform alkali dissolution on the soybean protein isolate to obtain a soybean protein isolate alkaline solution;
[0035] (2) Keep the temperature of the above soybean protein isolate alkaline solution at 50°C, add Alcalase protease according to 5‰ of the weight of soybean protein isolate, stir for 3 hours, and when the degree of hydrolysis DH=20%, adjust the pH to 4.0 to inactivate the enzyme, 3200r / min centrifugation for 18min, the obtained supernatant is the crude soybean peptide liquid, the sugar content of the crude soybean peptide liquid is 10BX, and the pH value is 8;
[0036] (3) Perform total reflux nanofiltration operation on the crude soybean peptide liquid at room temperature, and perform adsorption and deodorizatio...
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