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Method for extracting ferulic acid from herba desmodii syracifolii

A technology for extracting ferulic acid from Desmodium stylifolia and ferulic acid, which can solve the problems of low solubility, cumbersome operation, and oil loss of aromatic compounds, etc.

Inactive Publication Date: 2018-02-02
桂林纽泰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the common solvent C0 2 It has the characteristics of non-polarity and small relative molecular mass, which makes the extraction effect unsatisfactory due to its low solubility and low selectivity for some substances. In actual operation, it is often necessary to increase the extraction rate by adding entraining agents. Finally, it is necessary to try to remove the entraining agent in the volatile oil, which will inevitably lead to the complexity of the process and the loss and oxidation of volatile components; in addition, because water is insoluble in CO 2 , so the supercritical C0 2 The extraction process often needs to pre-dry the raw materials, which increases the cost and causes the loss of aromatic compound oil
In addition, when extracting volatile oils from plants, due to supercritical CO 2 It has similar solubility not only to volatile oils but also to low-polarity compounds such as waxes, fatty acids, colored substances and resins in the stratum corneum. These substances will also be extracted during the extraction process, although complex systems can also be used. Pure volatile oil, but the operation is cumbersome and the requirements for equipment are more stringent

Method used

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  • Method for extracting ferulic acid from herba desmodii syracifolii

Examples

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Effect test

Embodiment 1

[0024] A method for extracting ferulic acid from Desmodium glabra, specifically comprising the steps of:

[0025] 1) Clean Desmodium sativa, dry, pulverize with a pulverizer, and cross a 20-mesh sieve;

[0026] 2) Subcritical water extraction: place the processed raw materials in an extraction kettle, add hydroxyethyl cellulose equivalent to 0.001 parts by weight of the raw materials, inject deoxygenated deionized water into the kettle at a flow rate of less than 10mL / min, and extract at a temperature of 160-170°C, pressure 8-10Mpa, extraction time 0.5h, extract Desmodium sativa, after the extraction is completed, the pressure is released, the extract flows into the collection tank and cooled to room temperature to obtain the extract;

[0027] 3) Purification: Concentrate the extract obtained in step 2), control the temperature ≤ 50°C, control the vacuum at -0.08±0.01MPa, concentrate to 0.95-1.05g / ml, perform column chromatography through DM130 macroporous resin, and load the ...

Embodiment 2

[0029] A method for extracting ferulic acid from Desmodium glabra, specifically comprising the steps of:

[0030] 1) Clean Desmodium sativa, dry, pulverize with a pulverizer, and pass through a 40-mesh sieve;

[0031] 2) Subcritical water extraction: place the processed raw materials in an extraction kettle, add hydroxyethyl cellulose equivalent to 0.001 parts by weight of the raw materials, inject deoxygenated deionized water into the kettle at a flow rate of less than 10mL / min, and extract at a temperature of 160-170°C, pressure 8-10Mpa, extraction time 1.0h, extract Desmodium sativa, after the extraction is completed, the pressure is released, the extract flows into the collection tank and cooled to room temperature to obtain the extract;

[0032]3) Purification: Concentrate the extract obtained in step 2), control the temperature to ≤50°C, control the vacuum at -0.08±0.01MPa, concentrate to 0.95-1.05g / ml, and perform column chromatography through AB-8 macroporous resin, T...

Embodiment 3

[0034] A method for extracting ferulic acid from Desmodium glabra, specifically comprising the steps of:

[0035] 1) Clean Desmodium sativa, dry, pulverize with a pulverizer, and cross an 80-mesh sieve;

[0036] 2) Subcritical water extraction: place the processed raw materials in an extraction kettle, add hydroxyethyl cellulose equivalent to 0.001 parts by weight of the raw materials, inject deoxygenated deionized water into the kettle at a flow rate of less than 10mL / min, and extract at a temperature of 160-170°C, pressure 8-10Mpa, extraction time 1.0h, extract Desmodium sativa, after the extraction is completed, the pressure is released, the extract flows into the collection tank and cooled to room temperature to obtain the extract;

[0037] 3) Purification: Concentrate the extract obtained in step 2), control the temperature ≤ 50°C, control the vacuum at -0.08±0.01MPa, concentrate to 0.95-1.05g / ml, perform column chromatography through D101 macroporous resin, and load the ...

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Abstract

The invention discloses a method for extracting ferulic acid from herba desmodii syracifolii. The method comprises the following steps: firstly, cleaning the herba desmodii syracifolii, drying in air,crushing the herba desmodii syracifolii by using a crusher, and sieving; secondly, carrying out subcritical water extraction: putting the treated raw materials into an extraction kettle, adding hydroxyethyl cellulose of which the proportion is equivalent to 0.001 weight part of the raw materials, injecting deoxygenated deionized water into the kettle, and extracting the herba desmodii syracifoliiat the extraction temperature of 160 to 170 DEG C and the pressure of 8 to 10Mpa for 0.5 to 1.0 hour; after extraction is ended, relieving the pressure, enabling an extracting solution to flow into acollecting tank, and cooling to room temperature to obtain the extracting solution; thirdly, purifying: concentrating the extracting solution obtained in the second step, carrying out column chromatography by macroporous resin, eluting with 50 percent v / v of ethanol to obtain an eluent, and concentrating and drying to obtain a finished product.

Description

technical field [0001] The invention relates to an extraction method in the field of biotechnology, in particular to a method for extracting ferulic acid from Desmodium sativa. Background technique [0002] Ferulic acid, also known as 3-methoxy-4-hydroxycinnamic acid, molecular formula, C 10 h 10 o 4 , Molecular weight: 194.19, is one of the derivatives of cinnamic acid (also known as cinnamic acid, 3 phenyl 2 acrylic acid, molecular structure). Ferulic acid can scavenge free radicals, promote the production of free radical scavenging enzymes, increase the activity of glutathione sulfur transferase and quinone reductase, and inhibit the activity of tyrosinase to regulate human physiological functions. Pharmacological effects: Anti-platelet aggregation, inhibit platelet 5-hydroxytryptamine release, inhibit platelet thromboxane A2 (TXA2) production, enhance prostaglandin activity, relieve pain, relieve vasospasm, etc. It is the basic raw material for the production of medi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C51/42C07C51/47C07C51/48C07C59/64
CPCC07C51/42C07C51/47C07C51/48C07C59/64
Inventor 谢冬养
Owner 桂林纽泰生物科技有限公司
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