A kind of method of extracting oosporin from Chaetomium chaetomium fermented liquid

Abstract

The invention belongs to the technical field of biology and particularly relates to a method for extracting oosporein from a chaetomium cupreumfermentation liquor. The method comprises the steps thatchaetomium cupreum is obtained through screening and separation and is fermented, filtered, concentrated, acidized, extracted, re-concentrated and crystallized, and the oosporein is finally obtained.The oosporein directly acts on plant diseases, the limitations of the chaetomium cupreum serving as a biocontrol bacterium for biological prevention and control are overcome, use of medication is accurate, plant damage is reduced, the dosage of an organic solvent for extracting the oosporein by using a direct extraction method is decreased, the energy consumption needed by oosporein extraction isreduced, the extracting efficiency is improved, the effect of conveniently and quickly obtaining high-purity oosporein at low energy consumption is achieved, and the method has a great popularizationand use value.

Description

technical field [0001] The invention belongs to the field of biotechnology. It relates to a method for producing oosporin crystals by fermenting microorganisms, in particular to a method for producing and preparing oosporin crystals by using Chaetomium chaetomium. Background technique [0002] Chaetomium belongs to the kingdom Eumycophyta, the phylum Ascomycota, the class Sordariomycetes, the order Sordariales, and the family Chaetomiaceae. [0003] Chaetomium is a common fungus that is widely distributed on various substrates in nature, such as plant residues, soil, animal manure, seeds and wood. [0004] For a long time, Chaetomium is a kind of microorganism that has been widely studied at home and abroad, not only Chaetomium has good antibacterial activity against various plant pathogenic bacteria. Moreover, Chaetomium also has a biocontrol effect. The earliest report can be traced back to 1954. Martin et al. found that after the Brazilian oat seeds were infected by Ch...

AI Technical Summary

Problems solved by technology

[0007] At present, in a report entitled "Purification and Identification of Antifungal Active Compounds Produced by Chaetomium CH-1", although a method for extracting oosporin from Chaetomium fermentation broth is proposed, But this method is to use organic solvent to extract directly, and needs to extract three times, operation is troublesome, and the amount of organic solvent is too large

In addition, because the final extraction effect was not directly given in the report, the applicant made several attempts based on the test method and found that the extraction rate was only about 70%, and the production capacity was extremely low, so it could not be put into production.