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High-purity total flavonoids of Scutellaria baicalensis and its preparation method and medicinal use

A total flavonoids of Scutellaria baicalensis, high-purity technology, applied in the direction of drug combination, pharmaceutical formula, plant raw materials, etc., to achieve the effect of inhibiting inflammatory response, high purity, and definite curative effect

Active Publication Date: 2021-05-04
FUDAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Studies have shown that flavonoids are the main chemical components and active ingredients in Scutellaria baicalensis, mainly in the form of glycosides or aglycones, of which baicalin has the highest content; studies have found that baicalin is the anti-inflammatory effect of Scutellaria baicalensis, especially in the treatment of lung cancer. One of the important active ingredients of internal inflammation, such as Pan Yongli found that baicalin can significantly reduce the pulmonary edema in rats with acute lung injury induced by lipopolysaccharide, and at the same time have a significant down-regulation effect on inflammatory mediators in lung tissue (Pan Yongli. Chinese Experimental Prescription Science Journal, 2013,19(3):238-240.); Baicalin also has a good therapeutic effect on pneumonia caused by influenza, can significantly reduce the inflammatory lesions of lung tissue, and significantly reduce the secretion of inflammatory cytokines in lung tissue , significantly down-regulated the level of lipid peroxidation products in lung tissue (Wan Qiaofeng, Gu Ligang, Yin Shengjun, et al. Chinese Pharmacology Bulletin, 2012,28(2):208-212.), but so far, for other flavonoids in Scutellaria baicalensis There are few reports on the relevant pharmacological effects of similar compounds

Method used

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  • High-purity total flavonoids of Scutellaria baicalensis and its preparation method and medicinal use
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  • High-purity total flavonoids of Scutellaria baicalensis and its preparation method and medicinal use

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Embodiment 1

[0023] Example 1 Preparation of high-purity Scutellaria baicalensis total flavonoids and characterization

[0024] (1) Preparation of total flavonoids from Scutellaria baicalensis

[0025] In the experiment of this example, firstly, the extraction and purification process of the total flavonoids of Scutellaria baicalensis was optimized. The experiment found that the total flavonoids content of 80% ethanol extract of Scutellaria baicalensis medicinal material was higher than that of water extract, 70% and 90% ethanol extract, and then 80% ethanol extract is extracted and processed with macroporous adsorption resin, the result shows that the content of total flavonoids after macroporous resin purification is higher than after extraction, and finally determine the extraction of total flavonoids of Scutellaria baicalensis described in the present invention according to the following method:

[0026] Take 1.2Kg of dried Scutellaria baicalensis, heat and reflux with 80% ethanol for ...

Embodiment 2

[0033] Example 2 Effect of high-purity Scutellaria baicalensis total flavonoids on the survival rate and body weight of acute lung injury mice

[0034] Ninety 5-week-old SPF male BALB / c mice were randomly divided into 6 groups (groups A, B, C, D, E, and F) according to body weight: group A was the normal control group, group B was the ALI model group , Groups C, D, and E are high-purity scutellaria baicalin total flavonoids with different doses, respectively 50, 100 and 200 mg / kg, and F is 100 mg / kg baicalin, 15 rats in each group. All mice were injected with propofol injection 0.026ml / 10g into the tail vein, and the mice were infected with 30 μl of H1N1 virus in the nose under anesthesia, 15 μl in each of the left and right noses, and the normal control group was given virus dilution. Two hours after infection, different concentrations of drugs were administered orally according to the groups, 0.1ml / 10g, for 7 days. Groups A and B were given 0.5% CMC-Na. From the day of infe...

Embodiment 3

[0042] Example 3 Effect of high-purity Scutellaria baicalensis total flavonoids on pneumonia in mice with acute lung injury

[0043] 48 5-week-old SPF male BALB / c mice were randomly divided into 6 groups (Groups A, B, C, D, E, and F) according to body weight: Group A was the normal control group, group B was the model group, Groups C, D, and E are different dose groups of high-purity Scutellaria baicalensis total flavonoids, 50, 100, and 200 mg / kg respectively, and F is 100 mg / kg baicalin, 8 mice in each group; all mice are injected with propofol injection through the tail vein 0.026ml / 10g, Infect mice with 30μl nasal drip of H1N1 virus under anesthesia, 15μl each in the left and right noses, among them, the normal control group was given virus dilution, and 2 hours after infection, different concentrations of drugs were given orally according to groups, 0.1ml / 10g , administration for 4 days, groups A and B were given 0.5% CMC-Na. 24 hours after the last administration, that ...

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Abstract

The invention belongs to the field of traditional Chinese medicines, and in particular relates to a high-purity total flavonoids from Scutellaria baicalensis and its preparation method and its application in the preparation of medicines for preventing and treating acute lung injury. The total flavonoids of Scutellaria baicalensis of the present invention is obtained by purifying the ethanol extract of dried root of Scutellaria baicalensis of Labiatae plant through macroporous adsorption resin, the product yield is 25.48%, the total flavonoid content is 83.00%, and the main flavonoid components include baicalin, Melaleuca Paper glucoside, wogonoside, baicalein, wogonin, chrysin, melaleucain, etc. The total flavonoids of Scutellaria baicalensis has been proved by the whole animal model experiment to have obvious therapeutic effect on acute lung injury in mice, and the therapeutic effect is obviously better than that of baicalin at the same dose (P<0.05). The high-purity total flavonoids of Scutellaria baicalensis of the invention can be used to prepare medicines for preventing and treating acute lung injury.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicines, and relates to high-purity total flavonoids of scutellaria baicalensis and its preparation method and its medicinal use, in particular to high-purity total flavonoids of scutellaria baicalensis and its preparation method and its application in the preparation of medicines for preventing and treating acute lung injury. Background technique [0002] The prior art discloses that acute lung injury (acute lung injury, ALI) is a lung injury caused by diffuse alveolar capillary membrane damage after various direct and indirect injury factors such as severe infection, trauma, radiation, shock, etc. Edema and micro-atelectasis are the pathological features of pulmonary inflammation and increased pulmonary permeability syndrome, which is the early stage of acute respiratory distress syndrome (Acute respiratory distress syndrome, ARDS). Studies have shown that ALI may be an immune disease with a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/539A61P11/00
CPCA61K36/539A61K2236/333A61K2236/51A61K2236/55
Inventor 陈道峰支海娟卢燕朱海燕
Owner FUDAN UNIV
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