Biotransformation method for L-glufosinate

Abstract

The invention discloses a biotransformation method for L-glufosinate. The biotransformation method comprises the following steps: composing a reaction system from a solvent and 2-carbonyl-4-(hydroxymethylphosphoryl)butyric acid used as a substrate, and then adding a biocatalyst, coenzyme, an additive and an ammonium salt into the reaction system for a biotransformation reaction so as to obtain a transformed solution containing L-glufosinate, wherein the biocatalyst is the whole cell of a genetically engineered bacterium co-expressed by glufosinate dehydrogenase derived from Saccharomyces cerevisia and formate dehydrogenase derived from Candida boidinii; the coenzyme is NADP+; and the ammonium salt is ammonium formate. The biotransformation method is simple in process flow, free of specialrequirements on equipment and suitable for industrial production. HPLC-MS and HPLC are employed for monitoring until the substrate is fully utilized.

Description

technical field [0001] The invention relates to a preparation method of pesticides, in particular to a biotransformation method of L-glufosinate-ammonium. Background technique [0002] Glufosinate-ammonium, the chemical name is 4-[hydroxy(methyl)phosphono]-DL-homoalanine, which was developed and produced by Hearst (now Bayer, Germany) in the 1980s, and belongs to phosphonic acid Herbicides are glutamine synthesis inhibitors, non-selective contact herbicides, which were registered for use as herbicides in 1984. In 2004, my country only had the registration of glufosinate-ammonium technical, and in 2005, my country had product registration. [0003] Since glyphosate was widely used, glyphosate-resistant weeds have been increasing, and the harm has gradually increased. Paraquat is a strong herbicide that kills weeds and is highly toxic to humans and animals. On July 1, 2014, my country revoked the registration and production license of paraquat water solution, and stopped pr...

AI Technical Summary

Problems solved by technology

The synthesis process is at 500-600°C. If the control is unstable, it is easy to produce yellow phosphorus and phosphine, which are very easy to be natural, which is very dangerous.

In addition, the material is highly corrosive, and there are strict requirements on the material selection and manufacturing process of the reaction equipment. The current domestic processing and manufacturing level is difficult to meet the production requirements.

[0005] These methods are all methods for preparing glufosinate-ammonium, glufosinate-ammonium is L / D mixed type, wherein the main role is L-type, and the effect of D-type is only 1 / 8 of L-type; L-glufosinate-ammonium can be used in soil It is degraded by microorganisms, but D-glufosinate-ammonium is difficult to degrade, which can eventually lead to soil compaction