Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Big data based identification method for aconitum transsectum DNA barcode and aconitum transsectum

A barcode and big data technology, applied in the field of molecular identification, can solve problems such as indistinguishability and difference in identification ability, and achieve the effects of high accuracy, short time-consuming and high repeatability

Active Publication Date: 2018-01-26
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
View PDF2 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Different gene fragments have great differences in the ability to identify different species. According to the inventor's previous research and existing research reports, the gene fragments matK, trnH-psbA and ITS have a certain ability to identify Aconitum species. However, it is still not possible to effectively distinguish Aconitum orthophylla from other commonly used medicinal plants of the genus Aconitum

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Big data based identification method for aconitum transsectum DNA barcode and aconitum transsectum
  • Big data based identification method for aconitum transsectum DNA barcode and aconitum transsectum
  • Big data based identification method for aconitum transsectum DNA barcode and aconitum transsectum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] 1. Collection and preservation of medicinal plant specimens of the genus Aconitum

[0041] Consult the literature and the collection information of major domestic herbariums, and formulate a field sampling strategy according to the sampling requirements of DNA barcodes. Each species collects as many individuals as possible in different places in its distribution area. The leaves of all individuals are dried and preserved with silica gel. for subsequent total DNA extraction. In the present invention, more than 40 kinds (including variants) of 130 leaf materials have been obtained. Among them, the samples of Aconitum aconitum came from 1 wild distribution area and 3 cultivation areas, a total of 12 samples. The list of materials and sample sources are listed in Table 1.

[0042] Table 1 Specimens and sources of medicinal plants of the genus Aconitum

[0043]

[0044]

[0045]

[0046]

[0047] 2. Total DNA extraction

[0048] The total DNA of the above-men...

Embodiment 2

[0075] Using the leaves of Aconitum aconitum and other plants of the genus Aconitum without flowers and fruits collected in medicinal planting areas and in the field as materials, the method in Example 1 was used for DNA extraction, PCR amplification and sequencing. The identification results showed that from the The psbK-psbI-sequence obtained from the known Aconitum plants is the base "ACTAAGA" at the 118-124bp position, and the base "CCTATCTATA" at the 213-222bp position, but the above-mentioned specificity is not found in other unknown plants of the genus Aconitum sex site.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a big data based identification method for an aconitum transsectum DNA barcode and aconitum transsectum, belonging to the technical field of molecular identification. By extensively investigating data information of field distribution areas, main cultivation areas, field sympatric species and closely related medicinal species, and then sampling in as many places as possibleto create a big database for DNA barcodes of samples, sequence information sites unique to aconitum transsectum are compared and found, and then the DNA barcode which can be used for identification unique to the aconitum transsectum is further obtained. The DNA barcode is a sequence with a total length of 443bp obtained by amplification of primer sequences SEQ ID NO. 1 and SEQ ID NO. 2, wherein 106-112bp is basic ACTAAGA, and 201-210bp is basic CCTATCTATA. The utilization of the DNA barcode can be greatly beneficial to the rapid identification of the medicinal plant aconitum transsectum.

Description

technical field [0001] The present invention relates to the technical field of molecular identification, in particular to a DNA barcode of Aconitum orientalis based on big data, and a method for identifying Aconitum orientalis by using the DNA barcode. Background technique [0002] Aconitum transsectum Diels, also known as Aconitum transsectum Diels, is a perennial herb of the genus Aconitum in the Ranunculaceae family. It is distributed in Yulong Mountain, Lijiang, in the northwest of Yunnan Province, and is often used in the folk to treat bruises. , Rheumatism and other diseases. The root tuber of Aconitum straight margin is the largest among the known medicinal Aconitum, and it has been cultivated in large quantities in recent years because it is rich in Bulleaconitine A. Aconitin is a diterpene alkaloid. It is the non-addictive analgesic drug acting on the peripheral nervous system with the best clinical curative effect and the largest difference between effective dose ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/6895
Inventor 杨俊波何俊王家艳王红李德铢
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com