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Bacillus velezensis capable of preventing cucumber fusarium wilt, and microcapsule slow release bacteria preparation thereof

A cucumber wilt and bacillus technology, applied in the direction of fungicides, bacteria, biocides, etc., can solve the problems of large amount of pesticide application, difficulty in functioning, high economic cost, etc., achieve high control efficiency, improve activity and stability, Portable effect

Inactive Publication Date: 2018-01-16
HUAZHONG AGRI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The application of biocontrol bacteria is restricted by various ecological factors such as the soil environment, and it is difficult to play a good role
For traditional pesticide dosage forms, due to the degradation, volatilization and leaching of drugs in the environment, the amount of pesticide applied is often larger than the actual required amount, and accordingly, the economic cost is also higher

Method used

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  • Bacillus velezensis capable of preventing cucumber fusarium wilt, and microcapsule slow release bacteria preparation thereof
  • Bacillus velezensis capable of preventing cucumber fusarium wilt, and microcapsule slow release bacteria preparation thereof
  • Bacillus velezensis capable of preventing cucumber fusarium wilt, and microcapsule slow release bacteria preparation thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Screening and Identification of Bacillus Velez NH-1

[0031] 1. Isolation of endophytic bacteria in cucumber seedlings

[0032] Endophytic bacteria in roots and stems were isolated from healthy cucumber seedlings in the cucumber wilt disease-affected area of ​​Zhengzhou, Henan. Weigh 0.5g of roots and stems for surface disinfection: 75% ethanol for 10-30s, rinse with sterile water for 2-3 times, 0.1% mercury liter for 1-3min, rinse with sterile water for 2-3 times, then add 5ml Grind well with sterile water, dilute 10 times and spread on LB plates, culture at 37°C for 48 hours, pick bacteria with different colony shapes to isolate and purify, and store the purified strains in glycerol tubes at -20°C.

[0033] 2. Screening of antagonistic endophytic bacteria

[0034] Inoculate the cucumber wilt pathogen Fusarium oxysporum on a PDA solid plate, culture at a constant temperature of 28°C for 2-3 days, pick a single colony of endophytic bacteria cultured at 37°C ...

Embodiment 2

[0043] Example 2 Bacillus Velez NH-1 Colonization Test in Cucumber Plants

[0044] 1. Screening of antagonistic bacteria resistant to rifampicin

[0045] Add 2 μg / ml rifampicin to LB solid medium, take 100 μl of Bacillus veleisi NH-1 bacteria solution, and culture at 37°C for 2-4 days. After the colony grows, select a single colony and transfer it to 5 μg / ml medium On the rifampicin-resistant plate, the resistant colonies were screened after culturing. Gradually increase the concentration of rifampicin to 10, 20, 40, 60 μg / ml respectively, and finally obtain the anti-60 μg / ml rifampicin strain. The resistant strains were continuously passaged 3-5 times on LB plates supplemented with 60 μg / ml rifampicin to stabilize the resistance of the resistant strains. The antagonistic ability of rifampicin-resistant strains to cucumber wilt was detected, and the resistant strains with similar antibacterial activity to the starting strain were selected for colonization research.

[0046]...

Embodiment 3

[0051] Example 3 Effects of Bacillus Velez NH-1 on the Defense and Protection Enzyme PAL, PPO, POD Activity in Cucumber Seedling Plants

[0052] When the cucumber seedlings grow to the three-leaf stage, select the cucumber seedlings with good growth, take the fermentation broth of the antagonistic strain NH-1 after 48 hours of fermentation, water 10ml of each plant, and then water 10ml of the fermentation broth for each plant after an interval of 24 hours, and again after an interval of 24 hours. Start to take the leaves to measure the enzyme activity. Three repetitions were set up, each with 3 seedlings, and the control group was irrigated with the same amount of distilled water.

[0053] 1. Determination of alanine ammonia lyase (PAL) activity

[0054] PAL activity was determined according to the literature method. Take 0.1g of the treated leaf tissue and add 3mL of buffer solution (5mmol / Lβ-mercaptoethanol, 1mmol / L EDTA, 1%PVP, pH 8.4), add a small amount of quartz sand i...

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Abstract

The invention discloses a Bacillus velezensis strain NH-1 capable of preventing cucumber fusarium wilt. The Bacillus velezensis strain NH-1 is preserved at China Center for Type Culture Collection, and the preservation number is CCTCC NO:M 2017336. The invention also discloses a microorganism bacteria preparation capable of preventing cucumber fusarium wilt. The active ingredient of the microorganism bacteria preparation is the Bacillus velezensis strain NH-1. The microorganism bacteria preparation is high in preventing and controlling efficiency, is safe, is economical, and is friendly to theenvironment; effect lasts long; and cucumber fusarium wilt prevention is realized via increasing the activity of protective enzymes in cucumber.

Description

technical field [0001] The invention belongs to the field of microorganism and agricultural disease prevention and control, and in particular relates to a strain of Bacillus velezensis resistant to cucumber wilt, and also relates to the application of the strain and a microbial agent containing the strain. Background technique [0002] Cucumber wilt, also known as dead seedling disease, is a soil-borne fungal disease. Fusarium wilt has a great impact on the yield and quality of cucumbers. After being infected, it will cause large-scale wilting of cucumber seedlings. In severe cases, the whole plant may die. Years can cause extinction. [0003] At present, there are still no very effective measures for the prevention and control of cucumber wilt. In production, the comprehensive prevention and control policy combining agricultural control, biological control and chemical control is mainly adopted to postpone the peak of Fusarium wilt to the later stage of harvest, thereby re...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01N25/28A01P3/00C12R1/07
Inventor 赵秀云罗文建史宣杰杨凡祁高富
Owner HUAZHONG AGRI UNIV
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