Method for extracting magnolol and honokioln from cortex magnoliae officinalis

Abstract

The invention provides a method for extracting magnolol and honokioln from cortex magnoliae officinalis. The method comprises the following steps: (1) pre-treatment; (2) ethanol extraction; (3) concentration; (4) water precipitation for impurities removal; (5) crystallization, wherein the pre-treatment in step (1) comprises the following steps: drying the cortex magnoliae officinalis and then cutting the dried cortex magnoliae officinalis into small segments with the length of 1-2cm; soaking the small segments into grape juice at a solid-liquid ratio of 1g: 10mL at room temperature for 4-5h and washing the soaked small segments with water after the soaked small segments are taken out; adding the washed small segments into ginger juice with the mass concentration of 50% at a solid-liquid ratio of 1g: (0.20-0.25)mL, soaking the washed small segments at the temperature of 80-85 DEG C for 2-3h after the washed small segments and the ginger juice are mixed uniformly; draining off water after the soaked small segments are taken out and fumigating the small segments with onions for 3-5h. According to the method, the cortex magnoliae officinalis is respectively soaked by the grape juice and fumigated with the onions for pre-treatment before the step of soaking the cortex magnoliae officinalis into the ginger juice and after the step of soaking the cortex magnoliae officinalis into theginger juice, and then extraction is performed, so that the obtained cortex magnoliae officinalis is not only high in extraction rate, but also good in purity; the purity of the cortex magnoliae officinalis can reach 99% or above through HPLC detection.

Description

technical field [0001] The invention belongs to the technical field of biological extraction, and in particular relates to a method for extracting magnolol and honokiol from magnolia bark. Background technique [0002] Magnolia officinalis is a perennial Magnoliaceae plant, as a commonly used clinical traditional Chinese medicine, its main active ingredients are magnolol, honokiol, isomagnanolol, tetrahydromagnolol, magnolol, etc., among which magnolol The content of phenol and honokiol is the highest. The Chinese Pharmacopoeia stipulates that the content of these two phenols can be used as medicine if the content of these two phenols reaches more than 2% in the medicinal material. Generally, the content of the trunk bark of a tree that has grown for about 15 years is between 3-5%. [0003] Magnolol and honokiol in magnolia bark have the functions of relaxing muscles, inhibiting the central nervous system, anti-inflammation, antibacterial, anti-pathogenic microorganisms, an...

AI Technical Summary

Problems solved by technology

Although this process can realize industrial production, the cycle is long and the cost is high; (4) Although silica gel column chromatography has a good separation effect on active ingredients and high product yield, it is very difficult for large-scale industrial production. Difficult to implement and high cost; (5) Supercritical CO 2 The extraction method and equipment are expensive, the initial investment is large, the cost is too high, and large-scale industrial production is difficult to achieve