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Extraction method of cucurbitane type triterpene compound and medical application in resisting Alzheimer's disease

A triterpene compound and cucurbitane-type technology, which is applied in the field of drug extraction, can solve the problems of side effects and reduced drug efficacy, and achieve the effect of low price, simple method and wide source

Active Publication Date: 2018-01-09
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, AD is a neurodegenerative disease, and the death of nerve cells caused during the course of the disease is irreversible, and these therapeutic drugs can only delay the pathological process, and the curative effect of the drug gradually decreases with the development of the disease, and there are side effects. Therefore, in recent years, new drugs have been sought Drugs and methods aimed at the etiology of AD have become the focus and difficulty of current research

Method used

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  • Extraction method of cucurbitane type triterpene compound and medical application in resisting Alzheimer's disease
  • Extraction method of cucurbitane type triterpene compound and medical application in resisting Alzheimer's disease
  • Extraction method of cucurbitane type triterpene compound and medical application in resisting Alzheimer's disease

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Effect test

Embodiment 1

[0041] Embodiment 1: Extract cucurbitane type triterpenoids in bitter melon fruit

[0042] A method for extracting cucurbitane-type triterpenoids from balsam pear fruits, comprising the following steps:

[0043] (1) After pulverizing 1.5kg (dry weight) of bitter gourd (Momordica charantia), extract it with 5L industrial grade methanol, place it in a shaker for 3 days with shaking at room temperature, vacuum filter, take the filtrate, and concentrate under reduced pressure to obtain Methanol extract 224g.

[0044] (2) Alternately use ethyl acetate and water to dissolve the sample of the methanol extract obtained in (1) and transfer it to a separatory funnel for distribution. After standing overnight, spin the ethyl acetate layer and the water layer to obtain ethyl acetate Layer sample 30g, water layer sample 170g.

[0045] (3) The sample of the ethyl acetate layer is separated by a silica gel open column (silica gel 200-300 mesh), and the elution system is n-hexane:acetone=99...

Embodiment 2

[0069] Embodiment 2: the biological activity analysis of compound 1-11

[0070] Studies have found that NGF can promote nerve growth and neuroprotection, can prevent or reduce neuronal degeneration, and to a certain extent can prevent the progression of AD. Because PC12 cells have the general characteristics of nerve cells, under the action of NGF, they will stop dividing, grow protrusions, and transform into neuron-like cells. Therefore, using PC 12 cells as an effective in vitro biological activity identification system to screen compounds with neuromimetic activity may become an effective drug for the treatment of Alzheimer's disease.

[0071] The analytical method includes the following steps:

[0072] (1) Culture of PC 12 cells (rat adrenal pheochromoma cell line): In a 100mm culture dish, add 10mL containing 20×10 4 The DMEM medium (containing 10% horse serum and 5% fetal calf serum) of each PC 12 cell was replaced after two days, and subcultured three days later. Whe...

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Abstract

The invention provides a cucurbitane type triterpene compound and an extraction method. The extraction method comprises the following steps: grinding bitter gourd fruit into powder; performing digestion in methanol; extracting with ethyl acetate and water to obtain crude extracts of water layer and ester layer; separating and purifying the crude extracts respectively to obtain the target compound.The cucurbitane type triterpene compound provided by the invention is a novel compound, the extraction method is simple and easy, and the yield is high. The cucurbitane type triterpene compound provided by the invention remarkably increases the neurite differentiation rate of PC12 cells in an in-vitro screening model resisting Alzheimer's disease, and can be used for preparing medicines and healthcare products resisting Alzheimer's disease. The invention provides a basis for new drug research and development as well as basic research against Alzheimer's disease, and has important practical significance. The structural formula of the cucurbitane type triterpene compound is shown in the specification.

Description

technical field [0001] The invention belongs to the field of medicine extraction, and relates to a method for extracting cucurbitane-type triterpene compounds, in particular to a method for extracting cucurbitane-type triterpene compounds 1-11 from bitter gourd fruit and its medical application for anti-Alzheimer's disease. Background technique [0002] With the development of society, the improvement of living and medical standards, the life expectancy of human beings is extended, resulting in an increase in the elderly population. According to the latest United Nations statistics on the world population, in 2015, the world's elderly population reached 901 million, accounting for 12% of the total population, and this proportion will continue to increase at a rate of 3.26% per year. It is estimated that by 2050, this number It will reach 2.1 billion, and the elderly population in major regions of the world will account for a quarter. The world has entered an aging society. ...

Claims

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Application Information

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IPC IPC(8): C07J71/00C07J9/00A61P25/28
Inventor 戚建华曹雪丽孙玉娟程丽红
Owner ZHEJIANG UNIV
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