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Probe used for multi-objective ion detection and application thereof

An ion detection, multi-target technology, applied in the field of ion detection probes

Active Publication Date: 2018-01-05
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] There have been many reports on the research of life- and environment-related ions by fluorescent probe methods, but most of them are single-probe single-target tests, that is, one probe can only detect one kind of ion, and there are few reports that can realize single-probe multi-target detection.

Method used

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  • Probe used for multi-objective ion detection and application thereof
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  • Probe used for multi-objective ion detection and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0101] 1. A probe used for multi-target ion detection, whose chemical structural formula is:

[0102]

[0103] Its synthetic route is as follows:

[0104]

[0105] The specific preparation method is:

[0106] 1) Synthesis of Intermediate 1: In a 100ml three-necked flask, add 27.36mmol of tris(2-aminoethyl)amine, 3.42mmol of rhodamine B and 60ml of absolute ethanol, reflux for 36h under nitrogen protection, and depressurize Ethanol was evaporated, extracted 3 times with 100ml dichloromethane respectively, the organic phase was dried overnight with anhydrous magnesium sulfate, and the solvent was evaporated to obtain a red viscous substance, which was separated by silica gel column chromatography, and the eluent was 9 / 1 / 1 methanol / chloroform / triethylamine to obtain 1.71g of colorless viscous intermediate 1; 1 HNMR (500MHz, CDCl 3 )δppm 7.89 (d, J=5.0Hz, 1H, ArH), 7.44-7.46 (br, 2H, ArH), 7.10 (bs, 1H, ArH), 6.40~6.42 (m, 4H, ArH), 6.27 (d ,J=5.0Hz,2H,ArH),3.34(q,J=10....

Embodiment 2

[0109] Embodiment 2. Reagent preparation

[0110] (1) Preparation of the probe solution: weigh 22.63 mg of the probe prepared in Example 1, dissolve it with acetonitrile, and prepare 25 mL of the probe stock solution with a concentration of 1 mM;

[0111] (2)Hg 2+ Preparation of stock solution: Weigh 90.7 mg of mercury perchlorate trihydrate, dissolve it with acetonitrile and prepare 10 mL of a solution with a concentration of 20 mM.

[0112] (3)Cu 2+ Preparation of stock solution: Weigh 74.12 mg of copper perchlorate, dissolve it in acetonitrile, and prepare 10 mL of a solution with a concentration of 20 mM.

[0113] (4) Fe 3+ Preparation of stock solution: Weigh 70.84 g of ferric perchlorate, dissolve it with acetonitrile, and prepare 10 mL of a solution with a concentration of 20 mM for later use.

[0114] (5) Other metal ions (Li + , Na + , K + , Ag + , Ca 2+ , Mg 2+ ,Sr 2+ , Ba 2+ , Zn 2+ , Cd 2+ ,Co 2+ , Ni 2+ , Pb 2+ , Al 3+ ) Preparation of the stock ...

Embodiment 3

[0119] Example 3. Fluorescence spectrometry on Hg 2+ , Fe 3+ 、Cu 2+ and F - detection

[0120] After adding the probe solution (1mM, 200μL) into a 10mL volumetric flask, dilute to the mark with acetonitrile / water so that the acetonitrile / water ratio is 97 / 3 (v / v), shake well, and take 3mL of the solution at a ratio of 1cm. In the color dish, use 470nm as the fluorescence excitation wavelength to measure the fluorescence spectrum. Add the probe solution (1mM, 200μL) to a series of 10mL volumetric flasks, and then add the metal ion Hg 2+ , Fe 3+ , Cu 2+ , Li + , Na + , K + , Ag + , Ca 2+ , Mg 2+ ,Sr 2+ , Ba 2+ , Zn 2+ , Cd 2+ ,Co 2+ , Ni 2+ , Pb 2+ , Al 3+ Stock solution (20mM, 0.2mL), dilute to the mark with acetonitrile / water, so that the acetonitrile / water ratio is 97 / 3 (v / v), shake well, take 3mL of the solution in a 1cm cuvette, and use 470nm as the The fluorescence excitation wavelength was used for fluorescence spectrometry.

[0121] Take the probe p...

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Abstract

The invention discloses a probe used for multi-objective ion detection and the application thereof. The probe is prepared mainly by the following raw materials: 3(2-aminoethyl) amine, Rhodamine B, trichloromethane, 4-chlorin-7-nitryl-benzofuroxan and 7-hydroxy-8-coumarin formaldehyde. According to the probe, single-probe multi-objective selective detection can be realized by controlling the detection time, so that the detection cost is low, and the detection efficiency is high. Additionally, the probe is beneficial to analysis on a complex microcosmic system.

Description

technical field [0001] The invention relates to a probe for detecting ions and its application, in particular to a time-resolved, multi-target ion detection probe and its application. Background technique [0002] The main methods for detecting transition metal ions and heavy metal ions are mostly based on atomic absorption, atomic emission, inductively coupled plasma mass spectrometry and chromatography. These traditional detection methods are expensive in equipment, complex in analysis process, cumbersome in sample processing steps, and require a large amount of sample , time-consuming, so that the wide application of the method is greatly limited, and it is more difficult to realize real-time, online, continuous monitoring. Fluorescent probes have been widely valued as a powerful detection tool in biological environment systems due to their high temporal and spatial resolution capabilities. The highly sensitive, efficient and time-domain analysis and detection of fluores...

Claims

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Application Information

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IPC IPC(8): C07D491/107C09K11/06G01N21/31G01N21/64G01N21/78
Inventor 曾晞方浚安廖贤
Owner GUIZHOU UNIV
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