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Culture device for lactic acid bacteria

A culture device and colony technology, applied in the field of microaerophilic microorganisms or obligate anaerobic microorganisms, cultivating and detecting microaerobic resistant microorganisms, can solve the problems of high cost, cumbersome and inconvenient device assembly and/or use, and eliminate the need , save the effect of incubation time

Active Publication Date: 2018-12-28
3M INNOVATIVE PROPERTIES CO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, these and other devices can be expensive to manufacture and may not be suitable for disposable
These devices can also be cumbersome to assemble and / or use

Method used

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  • Culture device for lactic acid bacteria
  • Culture device for lactic acid bacteria
  • Culture device for lactic acid bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0184] Embodiment 1. The preparation of the culture device that produces anaerobic environment independently .

[0185] Construction basis figure 1 A culture device that autonomously generates an anaerobic environment. The base was made of 5 mil (0.127 mm) thick polyester film (MELINEX grade 377, biaxially oriented polyester (PET) film, available from DuPont Teijin; Chester, VA). )composition. The nutrient powder formulation (listed in Table 1) and guar gum (16 g / L) were stirred in deionized water to achieve a substantially homogeneous mixture. The pH of the mixture was targeted at 5.8 + / - 0.5, adjusted with acid or base if necessary to meet the target. This mixture was knife coated onto a substrate and dried in a convection oven at 210°F (98.9°C) for 8 minutes as described in US Patent 4,565,783. Apply the nutrient layer to a target coat weight (after drying) of 0.56g / 24in 2 (3.6mg / cm 2 )thickness of. After drying, approximately 20 mil (0.508 mm) thick polyethylene f...

Embodiment 2

[0191] A culture device autonomously producing an anaerobic environment was prepared as described in Example 1, except that instead of coating with the powder mixture of Example 1, guar gum (89.7% by weight), sodium ascorbate (10% by weight) and carbonic acid A powder mixture of sodium hydrogen (0.3% by weight) coated the binder of the protective layer.

Embodiment 3

[0193] A culture device autonomously producing an anaerobic environment was prepared as described in Example 1, except that instead of coating with the powder mixture of Example 1, guar gum (89.5% by weight), sodium ascorbate (10% by weight) and carbonic acid A powder mixture of sodium hydrogen (0.5% by weight) coated the binder of the protective layer.

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Abstract

The present disclosure provides a culture device for counting colonies of microorganisms. The device can include a base, a protective layer, and a non-porous spacer member disposed therebetween. The spacing member includes holes defining growth compartments. Disposed in the growth compartment is a cold water soluble gelling agent, a dry oxygen scavenging reagent, a dry buffer system, and an effective amount of a dry carbon dioxide generating reagent. The buffer system is selected such that an aqueous mixture with a pH of less than or equal to 6.35 is formed when the growth compartment is hydrated with a predetermined volume of deionized water.

Description

[0001] Cross References to Related Applications [0002] This patent application claims priority to US Provisional Patent Application 62 / 154,312, filed April 29, 2015, the disclosure of which is incorporated herein by reference in its entirety. Background technique [0003] Many bacteria are sensitive to oxygen and will not grow in the presence of oxygen. It may be useful to determine the viability of such anaerobic microorganisms in a variety of environments. For example, it may be important to determine the presence of anaerobic microorganisms in food and beverage processing and / or packaging facilities. It may also be important to determine the presence of anaerobic microorganisms in healthcare settings, for example, using diagnostic assays to determine the presence of pathogens. As another example, water treatment facilities test water samples for the presence of such microorganisms. [0004] A variety of devices are available for culturing microorganisms. For example, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/34C12M1/00C12Q1/04
CPCC12M23/04C12M23/20C12M25/06C12M41/34C12M41/36C12Q1/04C12N1/20C12P7/56
Inventor 贾森·W·比约克埃文·D·布鲁蒂内尔亚当·J·斯塔内纳斯
Owner 3M INNOVATIVE PROPERTIES CO
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