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Molecular imprinting fluorescent microsphere array detection card and application thereof

A technology of molecularly imprinted microspheres and fluorescent microspheres, applied in the fields of material science and chemical analysis and detection, can solve the problems of cumbersome post-processing, and achieve the effects of shortening adsorption time, good biocompatibility, and large surface area.

Inactive Publication Date: 2017-12-29
SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the early molecularly imprinted polymers were bulk polymers prepared by bulk polymerization, and the post-processing was cumbersome.

Method used

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  • Molecular imprinting fluorescent microsphere array detection card and application thereof
  • Molecular imprinting fluorescent microsphere array detection card and application thereof
  • Molecular imprinting fluorescent microsphere array detection card and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 (1) Preparation of Acephate Molecularly Imprinted Microspheres (MIPs)

[0032] Weigh 0.02g sodium dodecyl sulfate (SDS), add 0.24mL dibutyl phthalate (DBP), 0.497g·mL -1 Polystyrene microspheres (Ps) microspheres 0.085mL, 10mL H 2 O, room temperature 25 ℃ 125rpm stirring swelling 15h, until the oil droplets disappeared. Add 0.1642g azobisisobutyronitrile (AIBN), 2.5mL toluene, 10mL4.8% polyvinyl alcohol (PVA) aqueous solution, 12.5mL H to the reaction system 2 O, stirring and swelling at room temperature 125rpm for 2h. Then add 0.1832g acephate, 0.344g methacrylic acid (MAA), 2.5mL ethylene glycol dimethacrylate (EDMA), 10mL4.8% PVA aqueous solution, 12.5mL H 2 O, stirring and swelling at room temperature for 2h. Finally, at 50°C, pass N 2protection, stirring at 180rpm for 24h, and the obtained particles were washed with methanol, hot water and tetrahydrofuran. After the reaction was completed, the obtained acephate molecularly imprinted microspheres (MI...

Embodiment 2

[0039] (1) Preparation of Acephate MIPs

[0040] Weigh 0.02g SDS, add 0.24mL DBP, 0.497g·mL -1 Ps microspheres 0.085mL, 10mL H 2 O, room temperature 25 ℃ 125rpm stirring swelling 15h, until the oil droplets disappeared. Add 0.1642g AIBN, 2.5mL toluene, 10mL4.8% PVA aqueous solution, 12.5mL H to the reaction system 2 O, stirring and swelling at room temperature 125rpm for 2h. In the reaction system, add 0.1832g acephate, 0.344g MAA, 2.5mL EDMA, 10mL4.8% PVA aqueous solution, 12.5mL H 2 O, stirring and swelling at room temperature for 2h. Finally, at 50°C, pass N 2 protection, stirring at 180rpm for 24h, and the obtained particles were washed with methanol, hot water and tetrahydrofuran. After the completion of the reaction, the obtained MIPs were centrifuged to remove the supernatant, and then methanol: acetic acid = 9:1 (v / v) was used as a solvent, and Soxhlet extraction was performed at 95° C. for 12 hours. The template molecules were removed in total. The polymer was ...

Embodiment 3

[0047] (1) Preparation of Acephate MIPs

[0048] Weigh 0.02g SDS, add 0.24mL DBP, 0.497g·mL -1 Ps microspheres 0.085mL, 10mL H 2 O, stirred and swelled at room temperature at 125 rpm for 15 hours until the oil droplets disappeared. Add 0.1642g AIBN, 2.5mL toluene, 10mL4.8% PVA aqueous solution, 12.5mL H to the reaction system 2 O, stirring and swelling at room temperature 125rpm for 2h. In the reaction system, add 0.1832g acephate, 0.344g MAA, 2.5mL EDMA, 10mL4.8% PVA aqueous solution, 12.5mL H 2 O, stirring and swelling at room temperature for 2h. Finally, at 50°C, pass N 2 protection, stirring at 180rpm for 24h, and the obtained particles were washed with methanol, hot water and tetrahydrofuran. After the completion of the reaction, the obtained MIPs were centrifuged to remove the supernatant, and then methanol: acetic acid = 9:1 (v / v) was used as a solvent, and Soxhlet extraction was performed at 95° C. for 12 hours. The template molecules were removed in total. The ...

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Abstract

The invention relates to a molecular imprinting fluorescent microsphere array detection card and application thereof. A fluorescent molecular imprinting microsphere array is adhered and fixed in a hard card to obtain the molecular imprinting fluorescent microsphere array detection card. The molecular imprinting fluorescent microsphere array detection card disclosed by the invention is high in recognition ability, high in adsorbing capacity, short in adsorption time, high in fluorescence intensity and stable, and the defects that the pretreatment is complicated and the sensitivity is low during the traditional acephate detection are overcome; ordered arrangement of the microsphere array is favorable for full contact between imprinting microspheres and targets, the defects that the microspheres are settled in a solution and the fluorescence intensity is influenced are overcome, an accurate, sensitive and rapid novel method is provided for detecting acephate pesticide residues, and a certain reference is provided for developing a detection technology for other types of pesticide residues.

Description

technical field [0001] The invention belongs to the field of material science and chemical analysis and detection, and specifically relates to a molecularly imprinted fluorescent microsphere array detection card and its application. Background technique [0002] Acephate (Acephate), also known as acephate, is a low-toxicity insecticide, but due to the increase in usage, the residue of acephate has also attracted people's attention. At present, the detection of acephate at home and abroad is limited to gas chromatography, but there are shortcomings such as cumbersome pretreatment and low detection sensitivity. [0003] Molecular imprinting technology is a technology for preparing materials with specific recognition ability for specific target molecules. It has shown broad application prospects in many fields such as biomimetic sensors, artificial enzyme catalysts, solid phase extraction, and pesticide residue detection. Among them, molecular imprinting technology has become ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6402G01N21/6428G01N2021/6432
Inventor 王颜红张红周强
Owner SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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