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Determination method of characteristic spectrum of Xiao shuan tong luo granules

A technology of characteristic atlas and Xiaoshuantongluo, which is applied in the field of determination of characteristic atlas of Xiaoshuantongluo granules, can solve problems such as inability to comprehensively reflect product quality status, lack of recording and measurement methods, etc., so as to ensure validity and consistency, Stable measurement conditions and comprehensive monitoring of effects

Active Publication Date: 2017-12-22
HEFEI PINGGUANG PHARMA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The quality standard of this product is recorded in the first part of the "Chinese Pharmacopoeia" 2015 edition. 1 Reference substance, rutin reference substance, borneol reference substance, through thin-layer chromatography to identify Xiaoshuantongluo granules, although the product quality can be detected to a certain extent, but the quality status of the product cannot be fully reflected; and the Pharmacopoeia also The determination method of the characteristic map of this product is not included

Method used

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  • Determination method of characteristic spectrum of Xiao shuan tong luo granules

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Embodiment 1

[0033] A method for measuring the characteristic spectrum of Xiaoshuan Tongluo granules, which adopts high performance liquid chromatography, and its chromatographic conditions include: the chromatographic column is an octylsilane bonded silica gel chromatographic column (250 × 4.6mm, 5 μm), and the mass fraction is 0.08wt% phosphoric acid aqueous solution was used as mobile phase A, acetonitrile mobile phase B was used, the detection wavelength was 262nm, the flow rate was 0.9ml / min, and the column temperature was 35°C for gradient elution;

[0034] The gradient elution process is: within 0-20min, the volume ratio of mobile phase A and mobile phase B changes gradually from 85:15 to 80:20 at a uniform speed; within 20-30min, the volume ratio of mobile phase A and mobile phase B changes from From 80:20 to 65:35 at a constant speed; within 30-45min, the volume ratio of mobile phase A and mobile phase B changes from 65:35 to 55:45 at a constant speed; within 45-50min, the volume o...

Embodiment 2

[0042] A method for measuring the characteristic spectrum of Xiaoshuan Tongluo granules, which adopts high performance liquid chromatography, and its chromatographic conditions include: the chromatographic column is an octylsilane bonded silica gel chromatographic column (250 × 4.6mm, 5 μm), and the mass fraction is 0.12wt% phosphoric acid aqueous solution is mobile phase A, with acetonitrile mobile phase B, detection wavelength is 258nm, flow velocity is 1.1ml / min, and column temperature is 25 ℃, carries out gradient elution; Described gradient elution process is the same as embodiment 1.

[0043] Sample preparation is the same as in Example 1.

[0044]Test operation: Take 50 μl samples of each reference substance solution and the test solution respectively, and record the chromatograms.

Embodiment 3

[0046] A method for measuring the characteristic spectrum of Xiaoshuan Tongluo granules, which adopts high performance liquid chromatography, and its chromatographic conditions include: the chromatographic column is an octylsilane bonded silica gel chromatographic column (250 × 4.6mm, 5 μm), and the mass fraction is 0.09wt% phosphoric acid aqueous solution is mobile phase A, with acetonitrile mobile phase B, detection wavelength is 261nm, flow velocity is 0.95ml / min, and column temperature is 32 ℃, carries out gradient elution; Described gradient elution process is the same as embodiment 1.

[0047] Sample preparation is the same as in Example 1.

[0048] Test operation: Take 10 μl samples of each reference substance solution and the test solution respectively, and record the chromatograms.

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Abstract

The invention discloses a determination method of a characteristic spectrum of Xiao shuan tong luo granules. A high performance liquid chromatography method is employed under the following chromatographic conditions: an octyl silane chemically bonded silica chromatographic column is employed, a phosphoric acid water solution is used as a mobile phase A and acetonitrile is used as a mobile phase B, detection wavelength is 258-262 nm, and gradient elution is carried out. The method can easily, quickly and accurately perform the determination of the characteristic spectrum of Xiao shuan tong luo granules under stable determination conditions. The method is more comprehensive in control on quality of the Xiao shuan tong luo granules and guarantees the effectiveness and uniformity of quality of the Xiao shuan tong luo granules.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicines, in particular to a method for measuring the characteristic spectrum of Xiaoshuan Tongluo granules. Background technique [0002] Xiaoshuan Tongluo Granule is a blood-regulating agent, which has the effects of promoting blood circulation, removing blood stasis, warming meridians and dredging collaterals. Clinically, it is mainly used for apoplexy caused by blood stasis blocking collaterals. Symptoms include sluggish expression, sluggish speech, cold hands and feet, and limb pain; ischemic stroke and hyperlipidemia patients. The prescription contains Chuanxiong, Salvia, Astragalus, Alisma, Panax notoginseng, Sophora japonica, Guizhi, turmeric, woody, borneol, and hawthorn, which are prepared through a series of processes. [0003] The quality standard of this product is recorded in the first part of the "Chinese Pharmacopoeia" 2015 edition. 1 Reference substance, rutin refer...

Claims

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 王康林桑晓斌马婕李明媛张成栋黄志生
Owner HEFEI PINGGUANG PHARMA
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