A method for treating rice planthopper eggs for microinjection

Abstract

The invention discloses a rice planthopper egg treatment method for microinjection. The method comprises the following steps that fresh rice planthopper eggs are collected; according to the principle that the heads and the tails of the eggs are in the same directions respectively, the eggs are sequentially arranged and transferred to a glass slide with an double-sided adhesive, the eggs are dried for 2-3 min, and then mosquito eggs are immediately covered with water-saturated oil to prevent further drying and water loss of the mosquito eggs; microinjection is conducted, the rice planthopper eggs obtained after injection are reserved after microinjection is completed, the rice planthopper eggs and the double-sided adhesive are put into pure water together, and culture is conducted for 2-3 days; when the rice planthopper eggs enter the red-eye period, the rice planthopper eggs are gently picked out and placed on wet filter paper, 2 days later, whether larvae are incubated or not is judged under an anatomical lens, and then the incubated larvae are transferred to tender shoots of rice seedlings. According to the method, natural non-infected wolbachia brown planthopper eggs serve as microinjection receptors, the infection rate is increased by 5-20%, and the egg passage efficiency is also improved by 80-100%.

Description

technical field [0001] The invention belongs to the field of pest control, and in particular relates to a method for treating rice planthopper eggs for microinjection. Background technique [0002] Rice planthopper, Homoptera Planthoppers (Delphacidae), commonly known as midges, fire midges, rattles. Harm rice and other crops by sucking plant juice. There are three main types of planthoppers that damage rice in my country: brown planthopper (Nilaparvata lugens Stal), White-backed planthopper (Sogatella furcifera (Horvath)) and gray planthopper (Laodelphax striatellus Fallén), among which the occurrence and damage of brown planthopper is the most serious, followed by white-backed planthopper. It mainly has the following hazards: (1) adult and nymph piercing and sucking hazards. Infected rice clusters in the field often start from spots or patches, and are yellower and shorter than normal rice plants from a distance, commonly known as "fake piercing", "yellow pond" or...

AI Technical Summary

Problems solved by technology

[0004] (3) Excrement often leads to mold growth, affecting photosynthesis and respiration of rice

This method is unstable for passage, and the infection rate is low