A method for judging the immune castration effect of chickens

A judging method and titer testing technology, which is applied in the field of judging the immune castration effect of chickens, can solve problems such as weight loss, wound infection, and no chicken castration effect, and achieve good stability, high accuracy, and guaranteed immunity. The effect of potency

Active Publication Date: 2019-03-19
成都瑞盛高科技股份有限公司
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  • Application Information

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Problems solved by technology

However, in addition to being "cruel", surgical "castration" has major disadvantages: it will cause bleeding, easily cause wound infection, and may even lead to animal death; it is very stressful to animals, which will cause food loss and weight loss. The breeding cycle is extended; the technology is primitive and labor-intensive, which is not suitable for modern intensive and large-scale production; the requirements for the operator's technical experience are quite high, and the range of applicable animals is also very limited; "Break resistance" and increase the burden on livestock owners
However, how to determine the immune castration effect of immunized animals is the focus of current research. In the prior art, there are reports on methods for determining the immune castration effect of other animals, but there is no method for judging the castration effect of chickens.

Method used

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  • A method for judging the immune castration effect of chickens
  • A method for judging the immune castration effect of chickens
  • A method for judging the immune castration effect of chickens

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1 prepares antigen

[0039] Prepare cLHRH protein (protein content is 6.75mg / ml, protein purity is 92%) by vaccine production test procedure with chicken luteinizing hormone releasing hormone recombinant antigen castration vaccine production strain, then dilute with normal saline, make its protein content respectively 0.25mg / ml, 0.5mg / ml, 0.75mg / ml, 1.0mg / ml, 2.0mg / ml, 3.0mg / ml and 4.0mg / ml, made into "water-in-oil-in-water" according to the vaccine emulsification process formula A small sample of the dosage form (the final concentration of the antigen is 0.025mg / ml, 0.05mg / ml, 0.075mg / ml, 0.1mg / ml, 0.2mg / ml, 0.3mg / ml and 0.4mg / ml), the physical properties and no After passing the bacterial test, it was refrigerated at 2-8°C and used for this test.

Embodiment 2

[0040] Embodiment 2 exploratory preliminary test

[0041] 1. Inject the final antigen concentrations of 0.025mg / ml, 0.05mg / ml, 0.075mg / ml, 0.1mg / ml, 0.2mg / ml, 0.3mg / ml and 0.4mg / ml antigen, the control group was injected with normal saline, 8 rats in each group, a total of 128 rats. Intramuscular injection, 0.5ml / feather.

[0042] 2. Observation records: Observe and record the external castration indicators such as sexual characteristics and sexual behaviors of chickens at each experimental stage, especially the unique changes in combs, wattles and feathers in the process of sexual maturity.

[0043] 3. Determination of serum antibody and sex hormone content: blood was collected 15 days, 30 days, 60 days, 90 days, and 120 days after immunization, serum was separated, and cLHRH antibody titer and testosterone and estradiol contents were measured.

[0044] Wherein, the content of testosterone and estradiol was determined by chemiluminescence detection kit (Siemens).

[0045] ...

Embodiment 3

[0112] Embodiment 3 repeats and expands test

[0113] According to the exploratory test results, in view of the reliability of the testicular weight suppression effect as an indicator of castration effect, and the stability and significance of the correlation between the 15-day serum antibody titer and the 120-day testicular weight suppression effect, we are in Jiujin Repeated and expanded verification tests were carried out on yellow and green-footed hemp chickens in order to further verify the stability and reliability of relevant technical parameters, and to explore the corresponding relationship between antibody titer and castration efficiency 15 days after immunization, so that, Provide more reliable technical parameters and supporting basis for the establishment of vaccine antibody efficacy testing methods and judgment standards.

[0114] 1. Inject antigens to 15-20 day-old nine-jin yellow commercial broiler chickens, and the dose of injected antigens is the same as in E...

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Abstract

The invention discloses a judgment method of a chicken immunity castration effect. The method comprises the following steps: diluting chicken lutropin releasing hormone antigens, then adding an adjuvant to emulsify, afterwards, immunizing broilers, measuring the valence of antibodies of chicken lutropin releasing hormone of the broilers, then observing appearance characteristics and measuring the weights of sex hormone and sexual gland, taking confidence intervals in which the valence of antibodies after immunizing for 15 days is not lower than 10*IC50 and the active suppression of testis weight after immunizing for 4 months is 95% as a judgment standard of 10*IC50 to judge the effective rate of castration. By researching the correlation of the valence of cLHRH antibodies with the appearance characteristics of immunized chicken, hormone content and the weight of the sexual gland, a judgment method of the chicken immunity castration effect is formulated, the method is good in stability and high in accuracy, and can fully guarantee the immunizing potency of castration vaccines within the immunizing period of validity.

Description

technical field [0001] The invention belongs to the technical field of castration effect determination, and in particular relates to a method for determining the immune castration effect of chickens. Background technique [0002] "Castration" is one of the major inventions in ancient my country, commonly known as "castration". It is a traditional animal husbandry production technology to improve meat quality. It has been used for thousands of years and has a decisive position in my country's animal husbandry economy and traditional food civilization. In addition to improving the meat quality, taste and flavor of livestock and poultry, castration can make animals docile, easy to manage, use, gain weight quickly, reduce calorie consumption, and improve feed utilization. However, in addition to being "cruel", surgical "castration" has major disadvantages: it will cause bleeding, easily cause wound infection, and may even lead to animal death; it is very stressful to animals, wh...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/74
CPCG01N33/74
Inventor 吉传义何信群伏刚杨建波李峰谢建勇龚文波方鹏飞严成郝伟伟潘华柱扶海
Owner 成都瑞盛高科技股份有限公司
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