Production technology of compound microorganism bacterium agent for resisting bacterial wilt of eggplant

A technology of compound microbial inoculum and production process, applied in the directions of biocides, chemicals for biological control, biocides, etc., can solve the problems of inability to achieve long-term disease resistance, waste of traditional Chinese medicine resources, and insignificant effects. To achieve the effects of enhancing plant stress resistance, high survival rate, and simple and easy production process

Inactive Publication Date: 2017-12-08
烟台丰成农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The prevention and treatment of eggplant bacterial wilt has a certain history, especially the biological control of eggplant bacterial wilt is the focus of current research, such as the patent application number 201010608576.8 of Nanjing Agricultural University, which can prevent and control eggplant bacterial wilt. Microbial organic fertilizer, using self-isolated Bacillus subtilis Ⅱ-36 to add to the organic fertilizer to make bio-organic fertilizer to prevent eggplant bacterial wilt, has the disadvantage of single strain and ineffective effect
Another...

Method used

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  • Production technology of compound microorganism bacterium agent for resisting bacterial wilt of eggplant
  • Production technology of compound microorganism bacterium agent for resisting bacterial wilt of eggplant
  • Production technology of compound microorganism bacterium agent for resisting bacterial wilt of eggplant

Examples

Experimental program
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Effect test

Embodiment 1

[0025] a) Preparation of Bacillus colloidal bacteria: the strain was purchased from Beina Bio-tech, strain number BNCC173119, strain culture medium 50kg, formula was peptone 0.5kg, beef extract 0.15kg, sodium chloride 0.25kg, pH7.5,121 Sterilize at ℃ for 30min, connect to purchase bacteria, culture at 150r / min in a shaker for 24h to obtain the culture liquid; prepare 1t amplification medium, the formula is 40kg beef extract, 9kg yeast extract powder, 3kg sodium chloride, pH7.5 , Transfer to the fermentation tank for high temperature sterilization, after sterilization, when the temperature is reduced to 36 ℃, insert 50kg of bacterial seed liquid, rotate at 120r / min for 26h, transfer out the fermentation broth 4000r / min and centrifuge for 30min to obtain Bacillus colloidalis Bacteria 128.7kg.

[0026] b) Preparation of Trichoderma harzianum: purchased from the strain, purchased from the Trichoderma Strain Collection and Management Center of Shanghai Jiaotong University, prepared th...

experiment example 1

[0033] Experimental example 1: Potted eggplant bacterial wilt prevention test

[0034] The composite microbial inoculum produced by the method of Example 1 was used for potted disease prevention test. The test steps are as follows:

[0035] The experimental eggplant variety "Zi Rong No. 2" was purchased locally. The bacterial wilt pathogen Ralstonia solanacearum was purchased from Beina biological number BNCC221120. The experimental soil was taken from Datian, Youyi Village, Laishan Town, Yantai City, which is prone to eggplant bacterial wilt. The soil, texture sandy soil, pH=5.3, organic matter 7.3g / kg, total nitrogen (N) 0.762g / kg, total phosphorus (P 2 O 5 )0.577g / kg, total potassium (K 2 O) 15.3g / kg. There are 3 treatments in the experiment, namely: T1: use Jinzhengda antimicrobial agent for root irrigation, the application rate is 3ml / m 2 ; T2: Use the fungus of the present invention for spreading, the application rate is 5g / m 2 ; T3: No inoculation, as a control. A three-sea...

experiment example 2

[0041] Experimental example 2: Field trial of eggplant bacterial wilt control

[0042] The composite microbial inoculum produced by the method of Example 1 was used for field trials. Field trials were conducted in Xiaohezi Village, Fushan District, Yantai City from April 2016 to September 2016. The test field was the incidence of bacterial wilt in recent years. For more serious plots, and after organic fertilizer application, the experimental eggplant variety is Fengyan 2. Two treatments were set up in the experiment, respectively: T1: Use the inocula of the present invention for spreading, the application rate is 5kg / 667m 2 ; T2: No inocula, as a control group. Each treatment is repeated 4 times, with a plot area of ​​100m 2 .

[0043] Observe the disease on 30d, 60d, 90d, 120d after planting, disease grade: 0 is asymptomatic on the leaf surface; 1 is wilted on the leaf surface below 1 / 4 of the plant; 2 is 1 / 4 to 1 / 2 leaf The surface is wilting; the level 3 is that the leaf surf...

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Abstract

The invention provides a production technology of a compound microorganism bacterium agent for resisting bacterial wilt of an eggplant. The production technology is characterized in that raw materials of a fertilizer comprise the following components: 15-20 parts of bacillus mucilaginosus, 15-20 parts of trichoderma harzianum, 15-20 parts of streptomyces microflavus, 10-35 parts of chitosan oligosaccharide, 10-15 parts of vegetable oil sludge, and 10-15 parts of plant ash; three probiotics are mixed by thallus preparation, material mixing, pelletizing of compound bacteria, coating of the vegetable oil sludge at the first layer, coating of plant ash at the second layer, and coating of chitosan oligosaccharide at the third layer; the three probiotics are separated from the antibacterial compound which is chitosan oligosaccharide and play each function to the maximum. According to the production technology, the antagonism and disinfecting effect of the microorganism to bacterial wilt and the induced resistance of chitosan oligosaccharide to the eggplant are utilized to realize the prevention and control of bacterial wilt; the compound microorganism bacterium agent has the advantages of being free from pollution, environmentally friendly, low in cost, and long-lasting in effect.

Description

Technical field [0001] The invention belongs to the technical field of fertilizer processing, and specifically relates to a production process of a composite microbial inoculum resistant to eggplant bacterial wilt. Background technique [0002] Eggplant, Solanum (scientific name: Solanum melongena L.) Solanaceae, Solanum plant. Eggplant erect branches herb to subshrubs, up to 1 meter tall, branchlets, petioles and pedicels are all branched by 6-8-(10), flat or short-stalked stellate hairs, branchlets are mostly purple (wild There are often prickles), and the hair will gradually fall off as it grows old. The leaves are large, ovate to oblong-ovate, and the petiole is about 2-4.5 cm long (wild prickly). The fertile flower is solitary, the flower stalk is about 1-1.8 cm long, and the hair is dense. The shape and size of the fruit vary greatly. The shape of the fruit is long or round, and the colors are white, red, and purple. Fruits can be eaten as vegetables. The roots, stems...

Claims

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Application Information

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IPC IPC(8): A01N63/02A01N43/16A01N25/12A01P3/00
CPCA01N25/12A01N43/16A01N63/10A01N2300/00
Inventor 熊廷珍
Owner 烟台丰成农业科技有限公司
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