Novel listeriosis selective separating medium, preparation method and application thereof

Abstract

The invention relates to the technical flied of biological examination, and especially relates to a novel listeriosis selective separating medium, a preparation method and an application thereof. A formula of the novel listeriosis selective separating medium comprises 4 g of beef brain infusion powder, 4 g of beef heart infusion powder, 5 g of peptone, 16 g of casein peptone, 5 g of sodium chloride, 2 g of sodium pyruvate, 2 g of glucose, 2.5 g of sodium bicarbonate, 1 g of phosphoglyceride, 12. 5 g of agar powder, 5 ml of a phosphatidylinositol solution with concentration being 0.1 g / ml, 5 ml of a pyrans glucoside solution with concentration being 0.02 g / ml, and 5 ml of a propolis solution with concentration being 0.0125-128.0 [mu]g / ml in each liter of distilled water. The method employs natural antibiosis substance propolis as a selective additive of the listeriosis selective separating medium, an improved basic medium is combined for usage, the background microbe growth in various food can be effectively inhibited, so that the growth of the listeriosis in the selective medium is good, the growth of 13 types of serotype bacterial strains of listeriosis is good, and the pathogenic bacteria can be accurately and sensitively detected in the various food.

Description

technical field [0001] The invention relates to the technical field of biological testing, in particular to a novel selective isolation medium for Listeria, a preparation method and an application. Background technique [0002] Listeria monocytogenes (Listeria monocytogenes) is a food-borne pathogen that can cause serious invasive diseases such as meningitis, sepsis, and miscarriage. It is susceptible to pregnant women, the elderly, newborns, and immunocompromised populations. In 2000, the pathogen was listed as a food-borne pathogen that must be inspected by the World Health Organization's food safety work plan, and it has become a huge hidden danger to the public health of consumers. [0003] The conventional detection method of Listeria monocytogenes mainly relies on the addition of selective enrichment solution after the sample is homogenized for selective enrichment culture, and then the selective enrichment culture is streaked on a selective separation plate to separat...

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Problems solved by technology

However, the existing selective separation plates cannot effectively isolate and screen pathogenic bacteria in various samples. Effectively inhibit the growth of background microorganisms, which will cause the pathogenic bacteria to be detected to be covered by background microorganisms and cannot be detected; 2. The background microorganism levels in various food samples, environmental samples and clinical samples are quite different, which will lead to no real meaning of universal 3. Different types of selective isolation media cannot effectively separate all 13 serotype strains of Listeria monocytogenes. It leads to inaccurate test results and omission of certain serotype strains in the sample; 4. Most of the selective additives in the selective isolation medium will cause adverse effects on the environment, even after high-pressure treatment, this negative effect still exists

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