A method for extracting, separating and purifying four main anthocyanins from blackcurrant pomace

Abstract

The invention relates to a method for extracting, separating and purifying four main anthocyanins from the blackcurrant residue. The object is to provide a simple, economic, green, eco-friendly, and high-yield method for extracting, separating and purifying four main high-purity anthocyanins from the blackcurrant residue. According to the method, the blackcurrant waste residue produced from industrial production is taken as a raw material, and homogenized pulverized coupling enzyme hydrolysis technology, negative-pressure cavitation reinforced extraction technology, macroporous adsorption resin concentration technology, medium-pressure quick-flash reverse chromatographic separation technology and low-temperature crystallization and recrystallization technology are adopted to obtain four anthocyanin monomer ingredients including delphinidin 3-O-glucoside, delphinidin 3-O-rutinoside, cyanidin 3-O-glucoside, and cyanidin 3-O-rutinoside and having the purity reach 95%. The method is abundant and available in raw materials, short in time consumption, less in solvent consumption, high in purity of obtained anthocyanins, low in production cost, and suitable for massive industrial production.

Description

Technical field [0001] The invention relates to a method for extracting, separating and purifying four high-purity anthocyanins from blackcurrant pomace, and belongs to the field of separation and purification of plant secondary metabolites. Background technique [0002] Black currant, scientific name black currant (Ribes nigrruu L.), commonly known as black bean fruit, alias black summer fruit, is a small deciduous shrub of saxifraga family (saxifragaceae) and tea banana (Ribes), mainly produced in my country In Northeast China and northern Xinjiang, the fruits are small dark purple-brown berries, which can be eaten or processed into juice, jam and other foods. Blackcurrant is rich in sugar, organic acids, multiple vitamins, anthocyanins, active minerals and special aromatic ingredients, and has high nutritional value and medicinal value. The total anthocyanin content is 3.5 mg / g of fresh fruit weight (Zhang Zhidong 2002). The currently known therapeutic functions of blackcurra...

AI Technical Summary

Problems solved by technology

So far, the methods for separating and purifying anthocyanin monomers generally use semi-preparative high-performance liquid chromatography or high-speed countercurrent chromatography. These methods generally have shortcomings such as expensive equipment and cumbersome methods, and cannot carry out large-scale separation and purification. It is difficult to be applied in actual production, which makes anthocyanin products lack in large quantities, and the price is very expensive

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