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Gelatin hybridized compound as well as preparation method and application thereof

A compound and gelatin technology, which is applied in the directions of inactive components of polymer compounds, drug combinations, microcapsules, etc., can solve the problems of difficult control of drug release process, renal excretion, and limited drug load.

Active Publication Date: 2017-10-31
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing nano-drug carriers either have poor tumor permeability due to their large size, or are easily excreted by the kidneys even though the resulting drug has good tumor permeability due to their too small size, or contain Insufficiency of limited drug dosage, or insufficient control of the drug release process, etc., so it cannot effectively treat cancer

Method used

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  • Gelatin hybridized compound as well as preparation method and application thereof
  • Gelatin hybridized compound as well as preparation method and application thereof
  • Gelatin hybridized compound as well as preparation method and application thereof

Examples

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preparation example Construction

[0099] The present invention also provides a preparation method of the compound, comprising the following steps:

[0100] 1) providing a first solution, a first mixed solution, an interface forming agent and a crosslinking agent, wherein,

[0101] the first solution comprises a first solvent and gelatin dissolved in the first solvent;

[0102] The first mixed solution comprises a second solvent and nanoparticles;

[0103] 2) mixing the first solution and the first mixed solution, and reacting to obtain the first reaction solution;

[0104] 3) adding the interface forming agent to the first reaction solution, and reacting to obtain the second reaction solution; and

[0105] 4) adding the crosslinking agent to the second reaction solution, and reacting to obtain the complex.

[0106] In another preferred embodiment, the nanoparticles and the gelatin are as described in the present invention.

[0107] In another preferred embodiment, the first solvent and the second solvent m...

Embodiment 1

[0148] Example 1 Purified gelatin

[0149] Weigh 2g of commercially available unpurified gelatin (dark yellow, purchased from sigma, model G-9382), add 50mL of ultrapure water to it, stir for 2 hours in a 60°C water bath, and then add 50mL of precipitant acetone , stirred at room temperature for 1 h, then removed the supernatant, added 50 ml of ultrapure water to it again, and dissolved it by heating in a water bath at 60 °C. After being completely dissolved, the purified gelatin (Gelatin) is obtained by aliquoting and lyophilizing. Unless otherwise specified, the gelatin used to construct the microspheres is all purified gelatin (Gelatin).

[0150] The purified gelatin was tested to be as high as 90% pure.

Embodiment 2

[0151] Example 2 Preparation of complex GL

[0152] ①Weigh 50 mg of purified gelatin, dissolve it in 5 mL of ultrapure water, and stir to dissolve at 60°C;

[0153] ②Weigh 20 mg of lithotomite (LP) with a particle size of 10-30 nm (its specific surface area is 100 m 2 / g-900m 2 / g), dispersed in 2mL of ultrapure water, ultrasonicated for 30 minutes for uniform dispersion;

[0154] ③ Quickly add 0.5-1 mL of the LP solution prepared in step ② to the Gelatin solution prepared in step ①, and react at 60°C for 10 minutes;

[0155] 4. add dropwise 20 mL of interface-forming agent acetone to the mixed solution prepared in step 3., and react at room temperature for 20 minutes after the dropwise addition;

[0156] ⑤ Add 50 μL of glutaraldehyde solutions (1-25 wt %) with different concentrations to the solution prepared in step ④ dropwise, and react at room temperature for 12 hours;

[0157] ⑥ Use a rotary evaporator to evaporate the acetone in the solution of step ⑤;

[0158] ⑦ ce...

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Abstract

The invention relates to a gelatin hybridized compound as well as a preparation method and application thereof and particularly discloses a compound. The compound contains nanoparticles and biodegradable gelatin, wherein the gelatin is cross-linked to the surfaces of the nanoparticles; and the particle size of the compound is from 20 nanometers to 500 microns. The invention further discloses the preparation method and application of the compound. The compound has the excellent performance of high drug loading capacity and tumor permeability and can be gradually released in tumor cells. The preparation method is simple in process, controllable and convenient for large-scale industrialization. The compound can be widely applied to the diagnosis and treatment processes of various diseases.

Description

technical field [0001] The invention relates to the technical field of biopharmaceuticals, in particular to a gelatin hybrid compound and a preparation method and application thereof. Background technique [0002] With the increasing environmental pollution caused by modern industry, cancer has become a major problem endangering human health, and more and more patients die because of incurable cancer. Small molecule anticancer drugs are commonly used anticancer drugs, which usually have certain targets (such as chromosome modifications, heat shock proteins, molecular chaperones and protein kinases, etc.) cells are more important. However, it has the disadvantages of fast metabolism in vivo, short maintenance of effective blood drug concentration, and low bioavailability. Therefore, in order to improve its efficacy, a larger dose or multiple doses are often required, and at the same time, due to its lack of selectivity, it can kill tumor cells and also produce strong toxic ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/50A61K9/51A61K47/42A61K47/04A61K49/00A61K31/704A61P35/00
CPCA61K47/02A61K49/0093A61K9/5057A61K9/5169A61K31/704
Inventor 刘昌胜李玉林王国营王金鹅袁媛孙懿
Owner EAST CHINA UNIV OF SCI & TECH
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