Rapid estimation method for determining synthesis proportion and chelation rate of microelement compound amino acid/small-peptide chelate through combination of spectrophotometric method and XRD (X-ray diffraction)

Abstract

The invention provides a rapid estimation method for determining the synthesis proportion and the chelation rate of a microelement chelate through combination of a spectrophotometric method and an XRD (X-ray diffraction) technology. The method provided by the invention is suitable for determining an appropriate material ratio when a complex is synthesized by compound amino acid / small peptides and transition metal ions; meanwhile, the content of microelement inorganic salt in any organic microelement chelate product can be detected rapidly and accurately, so that the chelation rate of the product can be further estimated. The method is rapid in implementation, strong in applicability and simple in operation, and has the practical value for a single amino acid microelement chelate product and a compound amino acid / small-peptide microelement chelate product, therefore, the method has significance for promoting the development of a feed microelement additive industry and the publishing of relevant industrial standards.

Description

technical field [0001] The invention belongs to the application fields of chemistry, chemical industry and biochemistry, relates to the field of preparation of feed trace element additives, in particular to the preparation of a compound amino acid / small peptide trace element chelate and a method for rapidly estimating the chelation rate thereof. Background technique [0002] Trace elements refer to some elements that account for less than 0.01% of the total mass of organisms and are necessary for organisms. Such as iron, zinc, copper, manganese, silicon, iodine, bromine, selenium, etc. No matter in aquaculture, animal husbandry or poultry farming industries, even for humans, trace elements are indispensable nutrients in the metabolism of these animals and play an irreplaceable role. Since the use of trace element additives in the 1950s, it has mainly experienced three development stages. The first stage is mainly inorganic salts. It was first discovered by British Barben t...

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Problems solved by technology

Dextran gel filtration chromatography is complex to operate, takes a long time to experiment, and has high requirements for experimental equipment. It needs to be equipped with an atomic absorption spectrophotometer. Moreover, this method has a small amount of sample injection, a large dilution factor, and a large coefficient of variation. The requirements for personnel are also very high, and it is not suitable for promotion to actual production. The feasibility of other different products needs to be further studied; the organic solvent extraction method is simple to operate, and the requirements for experimental equipment and personnel are also low. However, theoretically, chelation Trace elements in the state also have certain solubility in organic solvents. For different products, choosing the appropriate organic solvent and dosage is still a problem that needs to be studied.

In short, the difficulty of determining the chelation rate of organic trace element chelate (complex) compounds lies in the following two aspects. One is that the products of organic trace element chelate (complex) compounds are abundant. There are relatively large differences in strength, and it is difficult to establish a unified method; the second is that it is difficult to accurately separate trace elements in the chelated state and free state in the sample, and the stability of organic trace elements to acids and alkalis is not strong. There is a certain degree of decomposition in the environment. Generally speaking, there is no mature separation method at present, and it is difficult to accurately measure the chelation rate.

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