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Blood genomic DNA extraction method

An extraction method and genome technology, applied in the field of DNA extraction, can solve the problems of large consumption of consumables, cumbersome steps, long waiting time, etc., and achieve the effects of simplifying operation steps, reducing consumption and preventing cross-contamination

Inactive Publication Date: 2017-10-13
CHANGSHA KINGMED MEDICAL DIAGNOSTICS INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The entire extraction process is cumbersome, consumes a lot of consumables, and waits for a long time

Method used

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Embodiment Construction

[0030] The following will clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only some of the embodiments of the present invention, rather than all the embodiments.

[0031] The blood genome DNA extraction kit that uses described blood genome DNA extraction method to comprise following components:

[0032] Cell Lysis Solution CL, Buffer GS, Buffer GB, Buffer BD, Buffer GDB, Washing Solution PWB, Elution Buffer TB, Proteinase K, Adsorption Column, Collection Tube, Centrifuge Tube.

[0033] The blood genome DNA extraction method comprises the following steps:

[0034] Step 1: Extract 200 μL of blood sample;

[0035] Step 2: Combine 200 μL of the buffer GB and 20 μL of the proteinase K into a premix, add the blood sample into the premix, shake and mix well to obtain the first mixture;

[0036] Step 3: the first mixed solution was placed at 56°C for 10 minutes, and at the same time mixed u...

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Abstract

The invention provides a blood genomic DNA extraction method. The blood genomic DNA extraction method comprises the steps of extracting a blood sample, adding into a premixed solution, raising the temperature, overturning, uniformly mixing, instantly centrifuging mixed liquid and the like. The blood genomic DNA extraction method provided by the invention solves the technical problems in the prior art that a DNA extraction method wastes time and labor, and the consumption of consumables is high.

Description

technical field [0001] The invention relates to the field of DNA extraction, in particular to a blood genome DNA extraction method. Background technique [0002] In current blood gene testing experiments, the common method is to purchase nucleic acid extraction kits for extraction or purification. However, the entire extraction process has to go through the process of lysate lysis→incubation→centrifugation→organic extraction (or passing through the column)→rinsing several times→drying→dissolving (or elution) to finally obtain DNA. The entire extraction process has cumbersome steps, consumes a lot of consumables, and waits for a long time. Contents of the invention [0003] In order to solve the technical problems of time-consuming and labor-intensive DNA extraction methods and large consumption of consumables in the prior art, the present invention provides a blood genome DNA extraction method that solves the above-mentioned problems. [0004] A blood genome DNA extracti...

Claims

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Application Information

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IPC IPC(8): C12N15/10
CPCC12N15/101C12Q2523/308
Inventor 唐笑贺乐刘岱璿周梅华代冰彭兴旺田倩何媛
Owner CHANGSHA KINGMED MEDICAL DIAGNOSTICS INST
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