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Compound enzyme used for extracting pueraria flavones, and method used for extraction with compound enzyme

A technology of pueraria flavonoids and compound enzyme is applied in the field of compound enzymes, which can solve the problems of long extraction time, low extraction rate, difficulty in large-scale industrial production and the like, and achieve the effects of simple extraction method, improved extraction rate and easy operation.

Inactive Publication Date: 2017-10-13
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The extraction rate of the traditional method is low, the extraction time is long, and the resources are not fully utilized
Ultrasonic-assisted extraction and microwave-assisted extraction to prepare total flavonoids of kudzu root are short, but due to large equipment investment, high operation and maintenance costs, it is difficult to be used in large-scale industrial production

Method used

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  • Compound enzyme used for extracting pueraria flavones, and method used for extraction with compound enzyme
  • Compound enzyme used for extracting pueraria flavones, and method used for extraction with compound enzyme
  • Compound enzyme used for extracting pueraria flavones, and method used for extraction with compound enzyme

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Take by weighing 3.0g pulverized pueraria root and cross 40 mesh sieves, add the disodium hydrogen phosphate-citric acid buffer solution of pH5.0 according to the liquid-to-solid ratio 30:1, add β-glucosidase (100IU / g)-pectinase ( 20000IU / g) compound enzyme, the amount of compound enzyme added is 350IU, the ratio of β-glucosidase enzyme activity to the total enzyme activity is 40%, and suction filtration after 1h in 45°C water bath. Add 70% ethanol to the filter cake according to the liquid-to-solid ratio of 20:1, extract under reflux at 80°C for 1 h, filter with suction, combine the filtrates before and after the two times, and measure the total flavonoids content of Pueraria lobata after mixing.

Embodiment 2

[0028] Weigh 3.0 g of kudzu root crushed and passed through a 40-mesh sieve, add 300 IU of β-glucosidase (100 IU / g), 300 IU of pectinase (20000 IU / g), and 300 IU of cellulase (30000 IU / g). Add pH 5 citric acid-disodium hydrogen phosphate buffer solution according to the liquid-to-solid ratio of 30:1, take a water bath at 50°C for 1 hour, and then filter with suction. During the suction filtration, add 70% ethanol to the filter cake according to the liquid-to-solid ratio of 20:1, extract under reflux at 80°C for 1 hour, and then filter with suction. The two filtrates were combined before and after, and the content of total flavonoids in Pueraria lobata was determined after mixing.

[0029] Determination of Pueraria flavonoids

[0030] (1) Drawing of standard curve

[0031] Accurately weigh 0.0010g of rutin, dissolve with 30% ethanol, and completely transfer to a 250mL volumetric flask to volume. Take this rutin standard solution 0, 2.5, 5.0, 7.5, 10.0, 12.5mL respectively in...

Embodiment 3

[0046] Example 3 β-glucosidase-pectinase compound enzyme treatment to extract pueraria flavonoids

[0047] 3-1 The effect of pH on the extraction rate of kudzu root flavonoids

[0048] (1) Weigh 3.0 g of kudzu root that is pulverized and passed through a 40 mesh sieve, and add disodium hydrogen phosphate-citric acid buffer solution with pH 4.0, 4.5, 5.0, 5.5 and 6.0 respectively according to the liquid-to-material ratio of 20:1, and the amount of compound enzyme added 300IU, β-glucosidase accounted for 50%, after 1h water bath at 50 ℃, suction filtration. Add 70% ethanol to the filter cake at a liquid-to-material ratio of 20:1, reflux at 80°C for 1 hour, and suction filter. The two filtrates were combined before and after. Pueraria flavonoids extraction rate as figure 2 It was shown that with the increase of pH, the extraction rate of Pueraria flavonoids first increased and then decreased, and the extraction rate of Pueraria flavonoids was the highest when the pH was 5.0. ...

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Abstract

The invention discloses a compound enzyme used for extracting pueraria flavones, and a method used for extraction with the compound enzyme. The compound enzyme comprises pectase and beta-glucosidase; the total enzyme dosage of pectase and beta-glucosidase ranges from 150 to 400IU; the enzyme activity of beta-glucosidase accounts for 20 to 80% of the total enzyme dosage. The method comprises following steps: radix puerariae is weighed, smashed, and sieved; a disodium hydrogen phosphate-citric acid buffer solution with a pH value ranging from 4.0 to 6.0 is added at a liquid-to-solid ratio of 10-40:1; pectase and beta-glucosidase are added; reaction is carried out at 40 to 60 DEG C for 0.5 to 2.5h; and pumping filtering is carried out so as to obtain a finished product. The advantages are that: the compound enzyme can be used for extracting pueraria flavones, extraction efficiency can be increased effectively, the method is safe, and is friendly to the environment, no pollution is caused, the method is simple, and operation is convenient.

Description

technical field [0001] The invention belongs to the field of compound enzymes, and in particular relates to a compound enzyme for extracting kudzu root flavonoids and an extraction method using the compound enzyme. Background technique [0002] Pueraria is an edible herb rich in flavonoids. Pueraria flavonoids have antibacterial, blood circulation, anti-cancer and anti-cancer effects. At the same time, it has various pharmacological effects such as lowering blood pressure, reducing myocardial oxygen consumption, promoting retinal blood circulation, inhibiting platelet aggregation, anti-arrhythmia, anti-oxidation and enhancing body immunity. main active ingredient. The extraction process is crucial to the yield of kudzu flavonoids. At present, the extraction methods of Pueraria flavonoids mainly include solvent extraction method and reflux extraction method. Because the traditional method of extracting flavonoids with water and organic solvents is to dissolve the active in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/42C12N9/18C12N9/26C12N9/88C07G99/00
CPCC12N9/18C12N9/2408C12N9/2437C12N9/2445C12N9/88C12Y301/01011C12Y302/01015C12Y302/01021C12Y402/02002C07G99/00
Inventor 张雪松朱媛曹正凡军民宋刚刘海燕
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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