Goat semen diluent used for relieving high temperature stress as well as preparation and application methods thereof

Abstract

The invention discloses a production method of frozen goat semen in a thin plastic pipe, belongs to the technical field of production of frozen livestock and poultry semen, and in particular relates to a production method of the frozen goat semen in the thin plastic pipe. The production method of the frozen goat semen in the thin plastic pipe comprises the following concrete steps: 1, preparing a frozen diluent; 2, collecting semen and carrying out microscopic examination; 3, diluting; 4, sub-packaging; 5, balancing; 6, freezing; and 7, unfreezing and rechecking. The production method of the frozen goat semen in the thin plastic pipe is simple and rapid in operation, complex instrument and equipment are not required, and the motility rate of unfrozen goat semen in the thin plastic pipe is not lower than 0.35 under the condition that the fresh semen quality meets the requirement, so that the motility rate requirement of artificial insemination on frozen semen can be met, the utilization rate of a ram also can be fully utilized, limitation of animal life is broken through, long-term storage of ram semen is realized, and application and popularization of the frozen goat semen are promoted to the utmost extent.

Description

technical field [0001] The invention relates to a diluent of goat semen for alleviating high temperature stress and a preparation method thereof, belonging to the technical field of livestock and poultry reproduction, and used for artificial insemination of goat semen in a high-temperature climate environment or diluted preservation at room temperature. Background technique [0002] Although goat semen cryopreservation technology has entered the stage of production and application, the low artificial insemination and conception rate of frozen semen is still one of the important factors that limit the production and promotion of goat frozen semen. In production practice, goat semen is still widely used in normal temperature dilution preservation and artificial insemination of fresh semen. The factors affecting the storage effect of goat semen at room temperature mainly include temperature, pH, dilution ratio and dilution method, among which high temperature stress is particul...

AI Technical Summary

Problems solved by technology

In high temperature and humid environment, the semen ejaculated from goat epididymis is prone to heat stress reaction, the metabolism and movement of sperm are accelerated, and a large amount of metabolic substrate is consumed; heat stress reaction is often accompanied by oxidative stress. During the storage of semen at room temperature, the content of acidic metabolites and reactive oxygen species (ROS) increases, resulting in a significant reduction in effective storage time and sperm motility