A kind of pseudomonas and its microbial preparation, preparation method and application

A technology of microbial preparation and Pseudomonas, applied in the field of environmental protection, can solve problems such as volatile organic compound pollution, and achieve the effects of clear composition, improved environment, and no secondary pollution

Active Publication Date: 2020-07-31
上海道多生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Aiming at the above-mentioned deficiencies in the prior art, the present invention provides a microbial agent for removing environmental odor and volatile organic compounds and a preparation method to solve the problem of odor in places such as landfills, garbage transfer stations, and newly decorated houses. Air pollution and volatile organic compound pollution

Method used

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  • A kind of pseudomonas and its microbial preparation, preparation method and application
  • A kind of pseudomonas and its microbial preparation, preparation method and application
  • A kind of pseudomonas and its microbial preparation, preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Screening and identification of Pseudomonas putida (Pseudomonas putida) Daoduo 2

[0034] Add soil samples polluted in chemical plants using biphenyl to the basal medium, add solid biphenyl for enrichment culture, culture on a shaker at 30°C at 200rpm / min for 8-12 days, then start subculture, the culture conditions are the same as above, every 2 -Transfer once every 5 days. After 8-12 times of transfer, the culture solution is properly diluted and cultured with inorganic salt solid medium to screen the strains that can grow. After continuous enrichment culture, the appropriate diluted bacterial solution was taken to coat the basic solid medium plate, and one of the bacteria grew rapidly. Pick this bacterial strain, utilize the colony of this strain bacterium smooth after LB plate culture, become dark white.

[0035] The strain was sent to the Institute of Microbiology, Chinese Academy of Sciences for strain identification. According to the comprehensive analy...

Embodiment 2

[0046] Embodiment 2 Deodorizing microorganism preparation and preparation method thereof

[0047] Deodorizing microbial agents, including Pseudomonas putida Daoduo 2, can be used to solve the problem of odor and VOC pollution.

[0048] The microbial preparation is prepared by fermentation of Pseudomonas putida Daoduo 2, the fermentation medium used is molasses medium, and the number of viable bacteria in the preparation is not less than 1×10 9 CFU / mL.

[0049] The preparation method of this deodorizing microbial preparation is as follows:

[0050] (1) plate culture

[0051] Inoculate Pseudomonas putida Daoduo 2 onto solid LB medium by streaking on a plate, and incubate in an incubator at 25°C to 35°C for 12 to 24 hours to form a single colony.

[0052] The solid LB medium contains 50-100 μg / mL ampicillin.

[0053] The solid LB medium was prepared according to the following weight percentages: 0.5% yeast powder, 1% peptone, 1% sodium chloride, 1.5% agar powder and the rest ...

Embodiment 3

[0061] Example 3 Using the above-mentioned microbial preparations for deodorization at a garbage transfer station in Tianjin

[0062] The deodorant bacterial agent of the present invention is diluted with tap water according to the ratio of 1:50, and is made into the deodorant bacterial agent of working concentration. Spray evenly on the surface of new and old garbage, and judge the treatment effect by detecting the concentration of odor and volatile organic compounds, and at the same time, judge the treatment effect through the senses.

[0063] Odor concentration is an index that reflects people's subjective perception of odor, and it is measured by the three-point comparative smelly bag method, which is dimensionless. Ammonia is measured by solution absorption-spectrophotometry, and volatile organic compounds are measured by gas chromatography, and the units are mg / m 3 , from a quantitative point of view to reflect the change of the odor caused by the ambient gas. Spray on...

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Abstract

The invention discloses a strain of Pseudomonas putida (Pseudomonas putida) Daoduo 2 and a screening method thereof, and also discloses a microbial preparation containing the strain, a preparation method and application of the microbial preparation. The microbial preparation of the present invention is used to remove odor and volatile organic compounds (VOC) in the environment, and its composition is stable and controllable, non-toxic, harmless and free of secondary pollution, and it can be used in garbage transfer stations, landfills, Newly renovated houses and other places can significantly improve the sense of polluted environment, can effectively reduce the concentration of odor and volatile organic compounds (VOC), and can continuously control the concentration of odor and VOC.

Description

[0001] Cross References to Related Applications [0002] This application is based on and claims priority from a prior Chinese patent application with application number 2017101046932 filed on February 24, 2017, the entire contents of which are hereby incorporated by reference. technical field [0003] The invention belongs to the technical field of environmental protection, and particularly relates to a pseudomonas, a screening method thereof, a microbial preparation containing the pseudomonas, a preparation method and an application of the microbial preparation. Background technique [0004] With the rapid development of our society, a series of environmental problems caused by urban domestic waste have attracted people's attention, especially in urban waste landfills and transfer stations, due to the stench and peculiar smell generated by the accumulation of waste, it will make people unhappy. It will seriously affect the production and life of nearby residents. The odo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20B01D53/84B01D53/44C12R1/40
CPCB01D53/44B01D53/84B01D2257/708B01D2257/90C12N1/20C12N1/205C12R2001/40Y02P20/59Y02A50/20
Inventor 唐鸿志王伟伟杨科武
Owner 上海道多生物科技有限公司
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