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Uses of mesembryanthemum crystallinum l. callus extract in delaying skin cell aging, nursing skin, treating and preventing skin cancer

一种愈伤组织、萃取物的技术，应用在冰花愈伤组织萃取物领域

Active Publication Date: 2017-09-26
BAIYUETE BEAUTY SKIN BIOTECHNOLOGY (SHANGHAI) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, melanocytoma is mainly treated clinically by surgical resection. As for patients who cannot afford surgical treatment, there is no effective treatment or prevention method

Method used

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  • Uses of mesembryanthemum crystallinum l. callus extract in delaying skin cell aging, nursing skin, treating and preventing skin cancer
  • Uses of mesembryanthemum crystallinum l. callus extract in delaying skin cell aging, nursing skin, treating and preventing skin cancer
  • Uses of mesembryanthemum crystallinum l. callus extract in delaying skin cell aging, nursing skin, treating and preventing skin cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1: the preparation of extract

[0042] The ice flower seeds (commodity code: 00K20) purchased from China Taiwan Vegetable Home Horticultural Materials Co., Ltd. were cultivated into ice flower plants, and the plants were divided into two batches, A and B. Wherein, batch A is subjected to following treatment, to provide ice flower callus:

[0043] 1. rinse the ice flower plant with 6% sodium hypochlorite solution, then rinse the ice flower plant with sterilized water, if necessary, repeat the aforementioned washing operation;

[0044] II. cut the wound on the surface of the ice flower plant to induce callus (during 1 to 3 months);

[0045] III. Under 25 ℃, 50% to 60% humidity, with MS medium (Murashige and Skoog BasalMedium, purchased from Sigma Company, product number: M5519) the callus that step II gains is cultivated (lasts 1 to 1.5 months) .

[0046] The ice flower plants of the batch B above and the ice flower callus obtained from the A batch of ice fl...

Embodiment 2

[0054] Example 2: Antioxidant Efficacy of Ice Flower Callus (IPC) Extract

[0055] Human dermal fibroblasts (CCD-966SK; purchased from ATCC) were cultured in MEM medium (purchased from Gibco, product number: 61100-061) for 24 hours. After that, the cells were divided into four groups and carried out as follows: deal with:

[0056] (1) Control group: the cells were cultured in MEM medium for 2 hours (that is, the cells were cultured in a culture medium without IP extract and IPC extract).

[0057] (2)H 2 o 2 Group: the cells were cultured in MEM medium for 1 hour (that is, the cells were cultured in a culture medium without IP extract and IPC extract), then, adding H 2 o 2 (to a final concentration of 1 millimolar in the culture medium) for 1 hour.

[0058] (3)H 2 o 2 +IP group: the cells were placed in the MEM medium containing 2 mg / ml of the ice flower plant (IP) extract obtained from Example 1 and cultivated for 1 hour, then, adding H 2 o 2 (to a final concentration...

Embodiment 3

[0062] Example 3: The effect of ice flower callus extract on delaying skin cell aging, conditioning skin, and repairing skin

[0063] (3-1) Treatment of human dermal fibroblasts

[0064] Human dermal fibroblasts (CCD-966SK; purchased from ATCC) were cultured in MEM medium for 24 hours, after which the cells were divided into four groups and processed as follows:

[0065] (1) Control group: the cells were cultured in MEM medium for 48 hours (that is, the cells were cultured in the culture medium without the extract of the ice flower callus).

[0066] (2) UVA+6hr group, UVA+24hr group, UVA+48hr group: the cells were placed in the MEM medium containing 2 mg / ml ice flower callus extract from Example 1 and cultivated for 6 hours, 24 hours hours, and 48 hours.

[0067] (3) Next, UVA was used to irradiate each group of cells above for 1 hour.

[0068] (3-2) Anti-photoaging and anti-oxidation

[0069] In order to further understand the anti-photoaging and anti-oxidation effects of...

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Abstract

Use of Mesembryanthemum crystallinum L. callus extract in the manufacture of a medicament or a skin care product, wherein the medicament or skin care product is for at least one of delaying skin cell aging, nursing skin, repairing skin, treating skin cancer, and preventing skin cancer.

Description

technical field [0001] The present invention relates to ice flower (Mesembryanthemum crystallinum L.) callus extract and application thereof, especially about using ice flower callus extract to delay skin cell aging, condition skin, repair skin, treat skin cancer, and / or prevent skin cancer application. Background technique [0002] Ultraviolet rays (UV) are one of the main factors causing skin aging and lesions. According to wavelength, they can be divided into long-wave ultraviolet (UVA), medium-wave ultraviolet (UVB), and short-wave ultraviolet (UVC). It is known that more than 90% of the ultraviolet rays in sunlight are UVA, which has strong penetrating power and can penetrate the dermis of the skin to cause damage to the skin. Frequent exposure to UVA radiation will cause skin photoaging (photoaging) ), reactive oxygen species (ROS) generation, DNA damage, collagen loss, skin relaxation, etc., may even cause skin cancer. [0003] Photoaging refers to the long-term exp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61P35/00A61P17/16A61K8/9789A61Q19/08A61Q19/00
CPCA61K8/97A61K36/185A61Q19/00A61Q19/08A61K8/9789A61P17/16A61P17/18A61P35/00A23L33/105A61Q17/04A23V2002/00A23V2200/318A61K9/0053A61K9/0095
Inventor 林咏翔郭玮文陈怡卉陈怡君施惠馨蔡云卿
Owner BAIYUETE BEAUTY SKIN BIOTECHNOLOGY (SHANGHAI) CO LTD
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