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Water-soluble fullerene nano-material as well as preparation method and application thereof

A technology of nanomaterials and fullerenes, applied in the field of water-soluble fullerene nanomaterials and its preparation method and application, to achieve the effects of general popularization and application, small dosage, and low drug cost

Inactive Publication Date: 2017-09-08
BEIJING FUNAKANG BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, one of the difficulties in the widespread application of embedded metallofullerenes is how to synthesize and separate them in large quantities.

Method used

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  • Water-soluble fullerene nano-material as well as preparation method and application thereof
  • Water-soluble fullerene nano-material as well as preparation method and application thereof
  • Water-soluble fullerene nano-material as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Embodiment 1, C 70 (OH) x (Amino Acid) y Synthesis

[0052] (1) Fullerene C 70 (OH) 29 Synthesis

[0053] C in toluene solution 70 Mix with a NaOH solution with a concentration of 50%, add a small amount of phase transfer catalyst tetramethylammonium hydroxide (TBAH), stir for 24 hours, add ethanol to precipitate the product, and dialyze to remove excess NaOH to obtain the product C 70 (OH) 29 , freeze-dried and stored.

[0054] (2)C 70 (OH) 13 (CH 2 OHCHNHCOOH) 4 Synthesis

[0055] The alkali solution of NaOH and serine (wherein the mass fraction of NaOH is 33%, the molar ratio of serine and NaOH is 1:4) is mixed with ethanol, and added dropwise to C 70 (serine and C 70 In a toluene solution with a molar ratio of 100:1), stir overnight (12h), remove the upper toluene layer, and dialyze the lower layer to remove excess NaOH, freeze-dry and store.

Embodiment 2

[0056] Embodiment 2, C 70 (OH) x (Amino Acid) y Number of modified hydroxyl groups and amino acids

[0057] (1) Determination of C 70 (OH) 29 hydroxyl number

[0058] According to elemental analysis (Flash EA 1112) result: C 53.55%, H 3.19%, Nfigure 1 shown) to calculate C 70 (OH) x number of hydroxyl groups. From thermogravimetric analysis, it can be known that C 70 (OH) x The solid powder contains 11.8% water, which is about 10 water molecules. Combined with the H content in the elemental analysis, it is estimated that the H content in the hydroxyl group is 1.87%. From this, the C can be calculated. 70 (OH) x The number of hydroxyl groups is 29.

[0059] (2) Determination of C 70 (OH) 13 (CH 2 OHCHNHCOOH) 4 The number of hydroxyl and serine

[0060] According to elemental analysis (Flash EA 1112) result: C 47.99%, H 3.21%, N 2.68%, combined thermogravimetric analysis, derivative thermogravimetric analysis curve (such as figure 2 shown) to calculate C 70 (O...

Embodiment 3

[0061] Embodiment 3, the mensuration of the hydrated particle size of water-soluble fullerene nanomaterial

[0062] Get a small amount of C prepared by Example 1 of the present invention 70 (OH) 29 and C 70 (OH) 13 (CH 2 OHCHNHCOOH) 4 The powder is dissolved in water to form a dilute solution. Due to the intermolecular interaction, C 70 (OH) 29 and C 70 (OH) 13 (CH 2 OHCHNHCOOH) 4 Agglomerates into nanoparticles in water to form particles with a hydrated particle size of 1-200nm.

[0063] The hydrated particle size of the nanoparticles in pure water at pH=7.0 was measured by dynamic light scattering (DLS, Zetasizer Nano ZSP), C 70 (OH) 29 and C 70 (OH) 13 (CH 2 OHCHNHCOOH) 4 The average particle diameters are 136.4±0.5nm and 134.7±0.4nm respectively, see image 3 .

[0064] A high-resolution atomic force microscope ( 4 BioScience AFM) determined C70 (OH) 29 and C 70 (OH) 13 (CH 2 OHCHNHCOOH) 4 The sizes of nanoparticles aggregated in water are about 40...

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Abstract

The invention discloses a water-soluble fullerene nano-material as well as a preparation method and an application thereof. A general structural formula of the water-soluble fullerene nano-material is shown as C2n(OH)x(Amino Acid)y, wherein n is less than or equal to 60 and is greater than or equal to 20, x is less than or equal to 50 and is greater than or equal to 0, y is less than or equal to 20 and is greater than or equal to 0 and Amino Acid is water-soluble amino acid. The nano-material provided by the invention is applied to preparation of medicines for treating tumors, wherein the tumors include at least one of liver cancer, lung cancer, colorectal cancer, renal cancer, pancreatic cancer, cancer in bones, breast cancer, ovarian cancer, prostatic cancer, esophagus cancer, gastric cancer, oral cancer, nose cancer, laryngeal cancer, cancer of biliary duct, cervical cancer, uterine cancer, testicle cancer, meningeoma, skin cancer, melanoma and sarcoma. The nano-material profvided by the invention is good in water solubility, good in affinity with organisms and low in preparation cost, and the nano-material can obviously block off tumor vessels and rapidly cut off nutrition supply to tumor tissues.

Description

technical field [0001] The invention relates to a water-soluble fullerene nano material and a preparation method and application thereof, belonging to the field of biomedicine. Background technique [0002] Cancer, also known as malignant tumor, is the abnormal proliferation and metastasis of cells caused by the loss of normal gene regulation of cells in local tissues under the stimulation of various carcinogenic factors. According to the "World Cancer Report 2014" published by the International Agency for Research on Cancer under the World Health Organization (WHO), cancer has become the main cause of global morbidity and death; in 2012, the number of new cancer patients worldwide increased by about 14 million In the same period, the number of deaths of cancer patients also reached 8.2 million; the report also pointed out that in the next 20 years, the number of new cancer patients each year may rise from 14 million in 2012 to 22 million. The current effective diagnosis an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K33/44A61K9/51A61K47/62A61K47/54A61K47/69A61P35/00A61N5/00
CPCA61K33/44A61N5/00A61N5/0601A61N5/0613A61N2005/0602A61N2005/0664
Inventor 王春儒周悦甄明明
Owner BEIJING FUNAKANG BIOTECH CO LTD
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