Bursin extraction method

Abstract

The invention discloses a bursin extraction method. The method includes: well mixing pretreated chicken bursa fabricius with water for injection, mashing and homogenizing; subjecting the homogenized bursa fabricius to twice extraction to obtain extract liquid, and adding beta-propiolactone for inactivation; hydrolyzing inactivated extract liquid to obtain hydrolysate; adopting a 0.22-0.45micron filter membrane for tangential flow microfiltration of the hydrolysate, collecting microfiltration permeation liquid, adopting a 1-5kD filter membrane for tangential flow ultrafiltration, and collecting ultrafiltration permeation liquid; adjusting an osmotic pressure of the ultrafiltration permeation liquid to obtain isoosmotic solution, adopting 0.1-0.22micron sterilization filter for sterilization, and collecting liquid namely bursin. The bursin extraction method is simple and convenient, the bursa fabricius utilization rate is increased, production cost is reduced, production time is shortened, and the extracted bursin can serve as an immunity enhancer, a feed additive, an auxiliary therapeutic agent and the like.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for extracting bursalin. Background technique [0002] Bursa of fabricus (BF) is the unique central humoral immune organ of poultry, which is equivalent to the bone marrow of mammals. There are many biologically active substances in BF, especially some small peptides with immunoregulatory effects, which can promote the differentiation and development of lymphocytes and the maturation of immune organs in birds and mammals. Generally, this type of small molecule bioactive peptides is called Farrell. Bursin (bursin, BS). In recent years, people have conducted extensive research on the biological function and mechanism of BS. It has been confirmed that most of BS have immunoregulatory functions, such as bursin tripeptide, the amino acid composition sequence is Lys-His-Gly-NH2, in which Lys:His:Gly:NH2 is 1:0.9:1:0.8, which can induce mammalian And the differentiat...

AI Technical Summary

Problems solved by technology

However, whether it is chemically synthesized or cloned, the development and application of BS peptide drugs have certain limitations.

First of all, the conformation is unstable, and natural linear peptides have good biological activity and stability in vitro, but the conformational changes caused by the molecular flexibility of linear peptides affect the strength and selectivity of binding to receptors; secondly, Low bioavailability, poor membrane permeability, easy to be degraded by enzymes, easy to remove, difficult to dissolve and easy to aggregate in some cases, make it difficult for peptides to have stable pharmacological activity in the digestive and circulatory systems The chemical synthesis method has high purity, but the cost is also high, and it is also easy to cause environmental pollution; the expression of a single BS gene through genetic engineering technology cannot meet the demand for quantity and type, and the endotoxin produced by bacteria or other hosts is difficult to remove and Purify the target protein, and it is difficult to obtain its double-stranded DNA due to too many tandem repeats, and the immunological activity of the expressed product needs to be further confirmed