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A method for efficiently inducing embryogenic callus of garlic using inflorescence rachis

A technology of embryogenic callus and callus, applied in the field of efficient induction of garlic embryogenic callus, can solve the problems of high cost of rapid propagation, low callus induction rate, long induction time, etc., and achieve abundant sources, The effect of shortening the culture cycle and the short culture cycle

Inactive Publication Date: 2020-08-04
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a method for efficiently inducing garlic embryogenic callus by utilizing inflorescence rachis to solve the problems of low callus induction rate, long induction time, and high cost of rapid propagation in the prior art

Method used

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  • A method for efficiently inducing embryogenic callus of garlic using inflorescence rachis
  • A method for efficiently inducing embryogenic callus of garlic using inflorescence rachis
  • A method for efficiently inducing embryogenic callus of garlic using inflorescence rachis

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Experimental program
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Effect test

Embodiment 1

[0038] In order to compare the effects of different explant types on the induction of embryogenic callus of different varieties of garlic, the experiment used inflorescence rachis, bulb disc (with shoot apex), leaf base and root tip of test-tube plantlets as materials, and the variety was 'Eshuizao ', 'Xuzhou White', 'January Early', 'Portuguese Garlic' and 'Early Mature Garlic'. Peel off the outer bract leaves of the inflorescence rachis, remove the degenerated flower primordium residues, membranous bracts and flower stem parts, cut them into 4 pieces longitudinally in a cross shape, and inoculate them on the same petri dish; the sterilized Garlic cloves remove storage leaves and vegetative leaves, cut off the pseudostem and blade at the top 6mm of the bulb disc, cut off the suberized tissue of about 1 mm close to the bottom of the stem disc, and obtain a stem disc with a germinated leaf base of about 5 mm in thickness (with stem tip), inoculate 4 per petri dish; cut the whit...

Embodiment 2

[0050] In order to compare the effects of different basic media on the induction of embryogenic callus of different varieties of garlic, MS, B 5 and MS+B 5 Organic medium, the test varieties are 'Ershuizao', 'Xuzhoubai', 'Zhengyuezao' and 'Zaoshu Garlic', and the hormone is 2.5 mg·L -1 2,4-D+0.5mg·L -1 KT. The method of inducing embryogenic callus using the inflorescence axis as the explant and the calculation method of the induction rate are the same as in Example 1.

[0051] The results are shown in Table 3: 'Ershuizao', 'Xuzhoubai', 'Zhengyuezao' and 'Zaoshu Garlic' in B 5 In minimal medium and in MS and MS+B 5 There was no significant difference in the healing time between the two organic basic media, but the four varieties in B 5 The callus induction rate on the culture medium was higher than that of other basal mediums, ranging from 88% to 90%, among which 'Eshuizao' was the highest at 90.18%.

[0052] Table 3 Effects of different varieties and basal medium on the...

Embodiment 3

[0056] In order to compare the effects of different hormone types and concentrations on the induction of garlic embryogenic callus, the cytokinin used in this experiment was KT (6-glycosylaminopurine), which was set at 0.1, 0.5, and 1.0 mg·L -1 3 concentrations, auxin 2,4-D set 1.5, 2.5, 3.5mg·L -1 The complete combination design of 3 concentrations, 9 treatments in total, 3 repetitions for each treatment, 10 culture dishes for each repetition, and 4 explants for each culture dish. The growth situation was observed every day and the callus induction rate was counted. The method of inducing embryogenic callus using the inflorescence axis as the explant and the calculation method of the induction rate are the same as the implementation case 1.

[0057] The results are shown in Table 4: the present invention preferably adopts 2.5mg L -1 2,4-D+0.5mg·L -1 The callus induction rate of KT was the highest, 90.18%, which was better than other combined concentrations, indicating t...

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Abstract

The invention discloses a method for highly-efficient induction of garlic embryogenic callus by utilizing a rachis. The method provided by the invention utilizes a garlic rachis as an explant to induce a somatic embryo for the first time, constructs an optimized system capable of obtaining a large number of garlic somatic embryos in a short period of time, and has the advantages of widely-available materials of the explant, and high efficiency, large quantity, rapid speed, structural integrity and good genetic stability of somatic embryo induction. In addition, through suspension culture of the somatic embryos, a culture period can be shortened; a rapid propagation system can be built; a good asexual propagation test system can be provided for breed improvement and transgenic acceptor and mutant selection; the somatic embryos are prepared into artificial seeds, so large-scale production of seedlings is realized; and large application values and wide development prospects are achieved.

Description

technical field [0001] The invention relates to plant tissue culture technology, and specifically designs a method for efficiently inducing embryogenic callus of garlic by using inflorescence rachis. Background technique [0002] Garlic (Allium sativum L.), also known as garlic and garlic, is a biennial herb of the genus Allium in the family Liliaceae. It mainly uses young leaves (garlic seedlings or green garlic), flower stems (garlic sprouts), and bulbs (garlic heads) as edible products. , high nutritional value, is one of the important economic crops with a long history of cultivation in my country, and is also an important vegetable for exporting foreign exchange. The vast majority of garlic cultivated in my country cannot form seeds, and mainly rely on bulbs for asexual reproduction. Its sterility also brings difficulties to the genetic improvement of varieties, which is not conducive to the selection of new varieties. The yield and quality of garlic are declining year b...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 吴震程雅琪刘敏蒋芳玲孔祥宇
Owner NANJING AGRICULTURAL UNIVERSITY
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