Respiratory tract micro-fluidic chip rapid detection technology and kit

Abstract

The invention is based on a micro-fluidic chip technology and performs rapid detection on seven respiratory tract pathogenic bacteria in combination with a nucleic acid isothermal amplification technology, and belongs to the field of safe and rapid detection of respiratory tract pathogenic bacteria. According to the technology, a sample subjected to rapid treatment is mixed with a reagent in a kit. The mixed liquid is added into a micro-fluidic chip. A primer pre-loaded into the micro-fluidic chip is subjected to a nucleic acid isothermal amplification reaction with a possible microbial gene template in the mixed liquid at certain single temperature. A fluorescent dye in the reaction system is combined with an amplification product to generate a fluorescent signal. Whether the seven pathogens exist in the respiratory tract or not is determined through instrument reading. According to the method, the need for the sample quantity is reduced through the micro-fluidic chip technology, the detection throughput is increased, and seven pathogenic bacteria can be detected at the same time. By adopting the nucleic acid isothermal amplification technology, the detection specificity and sensitivity are improved; the operation is simple, and the reaction time is short. A simple and rapid respiratory tract pathogenic bacteria detection technology is provided for clinical use.

Description

technical field [0001] The technology belongs to the field of rapid detection of respiratory pathogenic bacteria. It involves a microfluidic chip rapid detection technology and nucleic acid isothermal amplification technology; specifically, it relates to a respiratory microfluidic chip rapid detection technology and a kit, specifically including methicillin-resistant Staphylococcus aureus, Staphylococcus aureus, large intestine Escherichia, Klebsiella pneumoniae, Pseudomonas aeruginosa, Acinetobacter baumannii, Stenotrophomonas maltophilia specific gene conservation sequence has specific primers and primer sets; also relates to the use of primers and primer sets Detection of Methicillin-resistant Staphylococcus aureus, Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Acinetobacter baumannii, Stenotrophomonas maltophilia by isothermal amplification method detection methods and detection kits. Background technique [0002] Lower respira...

AI Technical Summary

Problems solved by technology

The probes and microspheres in this scheme are all expensive raw materials, and the price of the fluorescent quantitative PCR instrument is relatively high, which requires trained professionals to operate, and some hospitals do not have relevant instruments

In addition, in the PCR process of this method, more heating and cooling processes are designed, which makes the entire experiment time period longer

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