Detection and application of prawn enterocytozoon hepatopenaei trehalose-6-phosphate synthase gene

A technology of phosphate synthase and hepatic enterocystosis, which is applied in application, genetic engineering, glycosyltransferase, etc., can solve the problems of inability to conduct quantitative research, insufficient sensitivity and specificity, etc.

Active Publication Date: 2017-09-01
THIRD INST OF OCEANOGRAPHY MINIST OF NATURAL RESOURCES
View PDF1 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these detection methods also have shortcomings, including only qualitative description of the pathogenic microorganism, incapable of quantitative research, insufficient sensitivity and specificity, etc. [6]

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Detection and application of prawn enterocytozoon hepatopenaei trehalose-6-phosphate synthase gene
  • Detection and application of prawn enterocytozoon hepatopenaei trehalose-6-phosphate synthase gene
  • Detection and application of prawn enterocytozoon hepatopenaei trehalose-6-phosphate synthase gene

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0035] Below in conjunction with specific embodiment, further illustrate the present invention. It should be understood that these examples are only used to illustrate the present invention and are not intended to limit the scope of the present invention. For the experimental methods that do not indicate the specific conditions in the following examples, usually follow the routine conditions such as the Molecular Cloning Laboratory Manual [7] (Sam Brook, Russell (author), Huang Peitang (translation), "Molecular Cloning Experiment Guide", Science Press, 2002, third edition.) The experimental conditions described in, or according to the reagent or instrument manufacturer suggested conditions.

[0036] Concrete steps of the present invention are:

[0037] 1. Extraction of total RNA from shrimp infected with Enterocystis hepatica

[0038] Take 100mg of prawn hepatopancreas tissue, add 0.6mL TRI Reagent (Molecular Research Center Company), grind the sample with a grinding rod, a...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides detection and application of a prawn enterocytozoon hepatopenaei trehalose-6-phosphate synthase gene and relates to prawn enterocytozoon hepatopenaei. Reverse transcription is performed through extracting the total RNA of a prawn infected with the prawn enterocytozoon hepatopenaei, and then the one trehalose-6-phosphate synthase gene is identified through specific primer amplification. According to gene sequence information, an optimal primer is designed and used for a real-time quantitative PCR method, so that a basis is provided for detecting a transcription expression level of the gene in a prawn. The transcription level of the prawn enterocytozoon hepatopenaei trehalose-6-phosphate synthase gene in the prawn is detected through the real-time quantitative PCR method, and the real-time quantitative PCR method can be used in the detection of prawn enterocytozoon hepatopenaei diseases. The method has the characteristics of being high in sensitivity and good in specificity, and the information and the method are provided for prawn disease prevention and control.

Description

technical field [0001] The invention relates to prawn hepatic enterozoa, in particular to the detection and application of the prawn hepatic enterozoa trehalose-6-phosphate synthase gene. Background technique [0002] Enterocytozoon hepatopenaei (EHP), also known as Enterocytozoa, is a microsporidia found in prawns in recent years, mainly infecting important cultured shrimps such as Litopenaeus vannamei and Penaeus monodon [1] . It was first isolated and named in 2009 from the slow-growing tiger prawn (Penaeus monodon ) farmed in Thailand [2] . [0003] In recent years, a high number of EHP-positive shrimps have been detected in shrimp seedlings and cultured shrimps in Zhejiang, Guangdong, Fujian and other provinces in my country, and the detection rate is increasing year by year. [3] . Shrimp infection with E. hepatica generally does not cause immediate death. Shrimp can eat normally, with obvious abnormalities in body color, intestines, and stomach. Some infected shrim...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N15/54C12N9/10C12Q1/68C12Q1/48C12Q1/04C12R1/90
CPCC12N9/1051C12Q1/6893C12Q2600/158C12Y204/01015
Inventor 施泓阮灵伟徐洵
Owner THIRD INST OF OCEANOGRAPHY MINIST OF NATURAL RESOURCES
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap