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Fluorescent carbon quantum dot and application

A carbon quantum dot and fluorescence technology, which is applied to fluorescent carbon quantum dots and its application in the detection of TNP and intracellular imaging, can solve the problems of large size of nanomaterials, low safety factor, and toxic applications, and achieves low cost, Easy operation, high sensitivity and selective effect

Inactive Publication Date: 2017-09-01
HARBIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

2,4,6-Trinitrophenol (TNP) is the first nitroaromatic compound developed in the world. It is highly explosive and has a low safety factor.
However, the large size or toxicity of some functionalized nanomaterials limit their applications.

Method used

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  • Fluorescent carbon quantum dot and application
  • Fluorescent carbon quantum dot and application
  • Fluorescent carbon quantum dot and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Preparation and Characterization of Fluorescent Carbon Quantum Dots

[0024] Put 1g of citric acid and 2g of urea into a stainless steel reaction kettle, and then add 10mL of dimethylformamide to it. After the reaction kettle is closed, the mixture is heated to 160°C. After the reaction is carried out for about 6 hours, the obtained brown solution is Cool to room temperature, add 20mL NaOH (50mg / mL), mix and stir for 1min, centrifuge at about 16000rpm for about 10min, collect the precipitate, dissolve it in water and continue centrifuging (16000r / min, 10min), repeat washing twice , remove residual salt, alkali and other substances, and freeze-dry the precipitate to obtain black solid powder, that is, carbon quantum dots.

[0025] The morphology was characterized by transmission electron microscopy (e.g. figure 1 As shown in A), the carbon quantum dots in the transmission electron microscope picture are small in size and evenly distributed. (Such as figure 1...

Embodiment 2

[0027] Example 2 Fluorescent carbon quantum dots detect TNP

[0028] (1) Optional

[0029] Colorimetric detection: Nine nitroaromatic compounds were detected under the same conditions, including: toluene (toluene), nitrobenzene (NB), m-nitrotoluene (3-NT), 2,4,6- Trinitrophenol (TNP), o-nitrophenol (2-NP), m-nitrophenol (3-NP), 2,4-dinitrotoluene (DNT), 2,4,6-trinitrotoluene (TNT) and p-nitrophenol (4-NP). (Such as image 3A) shows that the ultraviolet absorption spectrum of the carbon quantum dot solution has an absorption peak at 548nm. After adding TNP, the carbon quantum dot absorption peak red shifts and a second absorption peak appears at 605nm. However, the UV absorption peaks did not change significantly after adding the same amount of other nitroaromatic compounds to the solution. (Such as image 3 In B), it can be seen that after adding TNP, the carbon quantum dot solution changed from the original pink to blue within a few seconds, while the solution mixed with...

Embodiment 3

[0034] Example 3 Cell fluorescence imaging experiment

[0035] Culture of Hela cells, a type of cell used in biological and medical research. Then use the thiazolium blue colorimetric method (MTT method) to test the toxicity of carbon quantum dots (such as Figure 6 ). It can be observed that different concentrations (0, 10, 20, 40, 50 μg / mL) of carbon quantum dots were cultured with Hela cells for 24 hours. After the culture was completed, the viability of Hela cells was tested, and the viability of the cells was strong. It shows that carbon quantum dots have low toxicity to cells. Compared with other biological probes, carbon quantum dots have good cell compatibility.

[0036] Carbon quantum dots have the characteristics of strong orange fluorescence, small particle size, high stability, good compatibility, and low toxicity, so try to apply carbon quantum dot solutions to cell imaging experiments. After the carbon quantum dots were cultured with Hela cells for 3 hours, th...

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Abstract

The invention discloses a fluorescent carbon quantum dot. The fluorescent carbon quantum dot is prepared by a method comprising the following steps: putting 1g to 2g of citric acid and 2g to 4g of urea into a reaction kettle; adding 10mL to 15mL of dimethylformamide and mixing; sealing the reaction kettle and heating the mixture to 150 DEG C to 180 DEG C; after reacting for 4h to 6h, obtaining a brown solution; cooling to room temperature; adding 20mL to 30mL of a 50mg / mL NaOH solution and mixing and stirring for 1min; centrifuging and collecting sediment; then dissolving into water and centrifuging; repeating for two times and freezing and drying sediment. The fluorescent carbon quantum dot emits orange light under an ultraviolet light source; after the fluorescent carbon quantum dot solution is mixed with TNP (Trisnonylphenylphosphite), a quenching phenomenon of fluorescent light occurs and the TNP can be detected through a colorimetric method or a fluorescence method; meanwhile, the solution is observed under a fluorescent lamp and becomes blue from pink; a detection result can be recognized by naked eyes; the fluorescent carbon quantum dot has high sensitivity and strong specificity and can be used for the field of cell imaging.

Description

technical field [0001] The invention belongs to the technical field of nano detection and polymer chemistry, and specifically relates to a fluorescent carbon quantum dot and its application in detecting TNP and intracellular imaging. Background technique [0002] In recent years, carbon quantum dots, as a new type of fluorescent nanomaterial, have many excellent properties: good photostability, good water solubility, dull pan-eye, up-conversion fluorescence properties and good biocompatibility, etc. Therefore, fluorescent carbon quantum dots have broad application prospects, including bioimaging, sensing, drug delivery, and photocatalysis. Compared with previous carbon nanomaterials, carbon quantum dots have unique luminescent properties. Especially in terms of sensors, such as biosensing for DNA and nitrite, as well as sensors for phosphate, glucose, α-fetoprotein, metal ion sensing or detection of explosives. Since nitroaromatic explosives are a serious source of water p...

Claims

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Application Information

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IPC IPC(8): C09K11/65C01B32/15G01N21/64B82Y40/00
CPCC09K11/65B82Y40/00C01P2004/04C01P2004/64C01P2006/60G01N21/643G01N21/6486G01N2021/6432
Inventor 柴芳任国娟曲凤玉王春刚苏忠民
Owner HARBIN NORMAL UNIVERSITY
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