A composition for treating ischemic stroke, its preparation method and medical application

A technology for ischemic cerebral apoplexy and a composition, applied in the field of medicine, can solve the problem that the treatment demand of ischemic cerebral apoplexy is far from being met, etc.

Active Publication Date: 2019-10-22
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] However, the clinical need for the treatment of ischemic stroke is still far from being met

Method used

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  • A composition for treating ischemic stroke, its preparation method and medical application
  • A composition for treating ischemic stroke, its preparation method and medical application
  • A composition for treating ischemic stroke, its preparation method and medical application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 Isolation and identification of prohematoxylin B and brazilian hematoxylin

[0038] 1.1 Preparation and separation:

[0039] Dried hematoxylin heartwood (21 kg), cut into thin strips, refluxed with 8 times, 6 times, 6 times of 95% ethanol for 3 times, each time for 1 hour; the extract was decompressed and the solvent was recovered to obtain 95% ethanol extract, After evaporating the solvent until there is no alcohol smell, suspend in water, extract with petroleum ether, ethyl acetate, and n-butanol in sequence, and recover the solvents respectively to obtain 60 g of petroleum ether extract, 1400 g of ethyl acetate extract, and n-butanol Extract 360g.

[0040] Take 800g of ethyl acetate extract, and go through silica gel (100-200 mesh) column chromatography, chloroform-methanol=30:1, 20:1, 10:1, 5:1, 1:1 gradient elution, TLC comparison and combination A total of 14 fractions of Fr.A-Fr.N were obtained. Fraction Fr.G was subjected to silica gel (200-300 me...

Embodiment 2-4

[0056] Example 2-4 a composition

[0057] Accurately weigh a certain amount of protohematoxylin B and brazilian hematoxylin solid powder according to the quality shown in Table 1, then mix the two, fully grind them in an agate grinding bowl to mix them evenly, and then obtain the target composition. Store at 4°C sealed and protected from light until use.

[0058] The composition of the composition of table 1 embodiment 2-4

[0059]

Embodiment 5

[0064] Example 5 Protective effect of the composition of the present invention on rat cerebral ischemia-reperfusion injury

[0065] 5.1 Test animals: SD rats, male, 8 weeks old. Provided by Victoria Liver Laboratory Animal Company. Animals in each group were fed under the same conditions, ambient temperature (23±2)°C, relative humidity 50%-60%, free access to food and water.

[0066] 5.2 Test drug:

[0067] 1) Protohematoxylin B

[0068] 2) Composition 1: the composition prepared in Comparative Example 1 (protohematoxylin B: brazilian hematoxylin=90:10)

[0069] 3) Composition 2: the composition prepared in Example 2 (protohematoxylin B: brazilian hematoxylin=70:30)

[0070] 4) Composition 3: the composition prepared in Example 3 (protohematoxylin B: brazilian hematoxylin=50:50)

[0071] 5) Composition 4: the composition prepared in Example 4 (protohematoxylin B: brazilian hematoxylin=30:70)

[0072] 6) Composition 5: the composition prepared in Comparative Example 2 (...

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Abstract

The invention provides a composition of protosappanin B and brasilin, and application thereof in resisting cerebral ischemic injury. Optimally, the mass ratio of the protosappanin B to the brazilin brazilin ranges from (80 to 20) to (20 to 80). According to the composition provided by the invention, through a synergistic effect of the two components, the cerebral ischemia area caused by ischemia reperfusion can be remarkably reduced, and the neurological score caused by cerebral ischemia is reduced.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a new composition for treating ischemic cerebral apoplexy, its preparation method and its medical application. Background technique [0002] Ischemic stroke is a serious cerebrovascular disease that causes acute neuronal necrosis due to decreased blood perfusion in certain areas of the central nervous system, which in turn causes irreversible damage to brain tissue and the entire neurological function. If the disease is not treated in time, it can cause a series of complications related to nerve damage, such as: dementia, blindness, aphasia, weakness of hands and feet, hemiplegia, etc. At present, ischemic stroke has the characteristics of high morbidity, high mortality, high disability rate and many complications in the elderly. With the improvement of people's living standards and the aggravation of population aging, ischemic stroke has become a major fatal diseas...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/352A61K31/335A61P9/10A61P25/00
Inventor 屠鹏飞曾克武赵明波
Owner PEKING UNIV
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