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New application of zymosan in resisting plant viruses

An anti-plant virus, zymosan technology, applied in the field of anti-plant virus pesticides and pesticides, to achieve the effect of no residue to produce drug resistance, not easy to produce drug resistance

Inactive Publication Date: 2017-09-01
NORTHWEST A & F UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Regarding the biological activity of zymosan, the predecessors believed that the polysaccharide has the functions of clearing heat and detoxification, anti-tumor, enhancing immunity and lowering blood pressure. In terms of anti-plant virus activity and its mechanism of action, there have been no relevant research reports at home and abroad.

Method used

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  • New application of zymosan in resisting plant viruses
  • New application of zymosan in resisting plant viruses
  • New application of zymosan in resisting plant viruses

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Example 1: Indoor therapeutic effect of zymosan on TMV virus.

[0014] (1) Experimental conditions: indoor potted tobacco was selected as the crop, and the variety was Nicotiana cordata; the target was TMV.

[0015] (2) Experimental design and arrangement: the experimental drug was zymosan, and 8% Ningnanmycin aqueous solution control drug and water control were set up. 3 treatments, repeated 3 times.

[0016] (3) Application method: Tobacco heart leaf grows to the 5-6 leaf stage and is used for the test. Using the half-leaf dead spot method, the entire leaf was first inoculated with TMV virus, and 2 hours after the inoculation, the left half of the leaf was smeared with zymosan or a control agent, and the right half of the leaf was smeared with water as a control.

[0017] (4) Investigation method: 5 days after applying the agent, count the number of dead spots on the left and right half leaves, and calculate the control effect according to the following formula:

...

Embodiment 2

[0022] Example 2: Field control effect of zymosan on tobacco mosaic virus disease.

[0023] (1) Experimental conditions: field tobacco was selected as the crop, and the variety was K326; the target was TMV.

[0024] (2) Experimental design and arrangement: the experimental drug was zymosan, and 8% Ningnanmycin aqueous solution control drug and water control were set up. 3 treatments, repeated 3 times, a total of 9 plots. The experimental plots were arranged in random blocks.

[0025] (3) Application method: Spray the pesticide 20 days after transplanting the tobacco, and spray the pesticide for the second time after every 7 days.

[0026] (4) Investigation method: Conduct drug efficacy investigation 14 days after the second application of the drug, and record the incidence according to the following standards:

[0027] Grade 0: The whole plant is disease-free; Grade 1: The new leaves show slight mosaic; Grade 2: One-third to one-half of the leaves show mosaic; Grade 3: One-...

Embodiment 3

[0033] Example 3: Field control effect of zymosan on wheat yellow dwarf virus disease.

[0034] (1) Experimental conditions: the crop is field wheat, the variety is Xiaoyan 6; the target is BYDV.

[0035] (2) Experimental design and arrangement: the experimental drug was zymosan, and 8% Ningnanmycin aqueous solution control drug and water control were set up. 3 treatments, repeated 3 times, a total of 9 plots. The experimental plots were arranged in random blocks.

[0036] (3) Application method: Spray the pesticide 20 days after the wheat turns green, and spray the pesticide for the second time after every 7 days.

[0037] (4) Investigation method: Conduct drug efficacy investigation 14 days after the second spraying, investigate 10 connected wheat plants at each point, investigate 250 plants in each plot, and record the incidence according to the following standards:

[0038] Grade 0: No disease in the whole plant; Grade 1: Yellowing of some leaf tips; Grade 2: Yellowing ...

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Abstract

The present invention relates to the new use of zymosan for anti-plant virus, especially relates to the use of zymosan to prepare medicine with anti-virus effect. The zymosan involved in the present invention has control effects on various plant virus diseases, such as tobacco mosaic virus disease, wheat yellow dwarf virus disease or rice stripe leaf blight virus disease and the like. The zymosan of the present invention can be prepared into a pharmaceutical composition by a method known in the prior art, and any pesticide pharmaceutically acceptable dosage form, such as water, soluble liquid, emulsifiable concentrate, microemulsion, suspension, emulsion in water, Wettable powder or water dispersible granule pharmaceutical composition, etc. In addition, zymosan has no toxic effects on humans, animals and the ecological environment, and is an ideal biological pesticide for the development of green and organic agriculture.

Description

technical field [0001] The invention relates to the technical field of pesticides, in particular to the field of anti-plant virus pesticides, and further relates to the new application of zymosan in anti-virus. Background technique [0002] Plant virus diseases are an important class of plant diseases. The global economic losses caused by plant virus diseases are as high as 60 billion U.S. dollars every year, of which the loss of food crops alone is as high as 20 billion U.S. dollars. Plant viruses do not have a complete cell structure, and they are absolutely parasitic to plants. In addition, plants themselves lack a complete immune system. Once a plant is infected by a virus, it will carry the virus for life and suffer continuous damage. Prevention and control posed great difficulties. At present, there is no specific anti-plant virus agent in agricultural production. Facing the serious damage of plant virus diseases and the shortage of anti-plant virus agents, it is of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/16A01P1/00A01P21/00
CPCA01N43/16
Inventor 不公告发明人
Owner NORTHWEST A & F UNIV
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