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Plant root growth promoting agent

A root-promoting agent and plant technology, applied in the direction of plant growth regulators, plant growth regulators, animal repellents, etc., can solve the problems of long growth cycle and high separation cost, and achieve the effect of promoting vigorous growth

Inactive Publication Date: 2014-01-08
TIANJIN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Lentinan has a variety of beneficial effects on animals and plants. It is generally extracted from fruiting bodies, which has a long growth cycle and high separation costs.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0004] 1. Prepare the mycelium seed solution of the acid-resistant mutant of Lentinus edodes.

[0005] (1) Activation of bacteria

[0006] Use an inoculation shovel to cut out 0.5 cm × 0.5 cm sized bacterial blocks from the shiitake mushroom strains stored on the slant medium of the test tube and inoculate them on the PDA medium on the inverted plate, and culture at a constant temperature of 25 ° C for 6 days.

[0007] (2) Liquid seeds for cultivating mycelia

[0008] Cut the activated slant strains into 0.5cm×0.5cm sized bacterial blocks and inoculate them in the liquid seed medium. Each 250mL triangular flask is connected with 5 bacterial blocks and 10 glass beads of uniform size. 100mL, cultured at 25°C, 150r / min for 6 days, and prepared as fermented seed liquid.

[0009] (3) Determination of MgSO secreted exopolysaccharide in medium 4 with KH 2 PO 4 Proportion

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PUM

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Abstract

The invention discloses a plant root growth promoting agent and belongs to the field of biochemical engineering. The method comprises the following steps: preparing a mycelium seed solution of a mushroom acid-resistant mutant; performing fermental cultivation on the mushroom mycelium, and producing a culture; precipitating or centrifuging the culture, and obtaining the supernatant; separating lentinan from the supernatant, or directly separating the lentinan from the mushroom; measuring composition and structure of sugar; and treating the plant root, stem, leaves or seeds through means of spraying, daubing and soaking or adding coating components by using 0.000001-100 mass percent of lentinan (containing neutral polysaccharides and acidic polysaccharides), so that an effect of promoting growth of the plant root system is achieved. The growth promoting agent is an edible mushroom fermented product and is safe and reliable to animals and plants. According to the growth promoting agent, vigorous growth of the plant root system can be effectively promoted, and the growth promoting agent has slight influence on other organs of plants, is not inhibited by high concentration of substances and is obviously superior to plant hormones or growth regulators. The product can be widely applied to the fields of agriculture and forestry, foods, environment and the like.

Description

technical field [0001] A plant root growth promoter. The product belongs to the field of biochemical industry. The lentinan produced by submerged culture of Lentinus edodes hyphae can effectively promote the vigorous growth of plant roots, and is not inhibited by its high concentration, which is obviously better than other plant hormones or growth regulators. The invention can be widely used in the fields of agriculture, forestry, food and environment to promote root growth of plants. Background technique [0002] Lentinan has a variety of beneficial effects on animals and plants. It is generally extracted from fruiting bodies, which has a long growth cycle and high separation costs. The use of acid-resistant mycelium to implement deep open culture can produce lentinan on a large scale; the advantage of low cost makes the use of lentinan in agriculture and other fields possible. The plant root system is a key organ for plant growth, and cultivation measures have been used...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/16A01P21/00C08B37/00
Inventor 李兴林张国运曹爱佳韩杨赵娜曹雪飞韩广建贾士儒
Owner TIANJIN UNIV OF SCI & TECH
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