Plant root growth promoting agent
A root-promoting agent and plant technology, applied in the direction of plant growth regulators, plant growth regulators, animal repellents, etc., can solve the problems of long growth cycle and high separation cost, and achieve the effect of promoting vigorous growth
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[0004] 1. Prepare the mycelium seed solution of the acid-resistant mutant of Lentinus edodes.
[0005] (1) Activation of bacteria
[0006] Use an inoculation shovel to cut out 0.5 cm × 0.5 cm sized bacterial blocks from the shiitake mushroom strains stored on the slant medium of the test tube and inoculate them on the PDA medium on the inverted plate, and culture at a constant temperature of 25 ° C for 6 days.
[0007] (2) Liquid seeds for cultivating mycelia
[0008] Cut the activated slant strains into 0.5cm×0.5cm sized bacterial blocks and inoculate them in the liquid seed medium. Each 250mL triangular flask is connected with 5 bacterial blocks and 10 glass beads of uniform size. 100mL, cultured at 25°C, 150r / min for 6 days, and prepared as fermented seed liquid.
[0009] (3) Determination of MgSO secreted exopolysaccharide in medium 4 with KH 2 PO 4 Proportion
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