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Zinc oxide nanoparticle preparation method based on silk fibroin bio-template regulation

A technology of zinc oxide nano and silk fibroin, which is applied in the field of biomedical materials, can solve the problems such as difficult to control the particle size and shape of zinc oxide nanoparticles, and achieve the effects of low cost, improved antibacterial performance, and small particle size

Active Publication Date: 2017-08-25
XUANWU HOSPITAL OF CAPITAL UNIV OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a method for preparing ZnO nanoparticles, which solves the problem that it is difficult to control the particle size and morphology of zinc oxide nanoparticles in the prior art

Method used

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  • Zinc oxide nanoparticle preparation method based on silk fibroin bio-template regulation
  • Zinc oxide nanoparticle preparation method based on silk fibroin bio-template regulation
  • Zinc oxide nanoparticle preparation method based on silk fibroin bio-template regulation

Examples

Experimental program
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Effect test

Embodiment 1

[0040] S1, dissolving silkworm cocoons after degumming to prepare silk fibroin aqueous solution; specifically:

[0041] S11, cut silkworm cocoons into pieces, weigh 5g of silkworm cocoons, and then boil them in 2L of 0.02mol / L Na2CO3 solution for 30min to remove sericin on the silk surface. After boiling, wash the degummed silk fibroin several times with deionized water and dry it naturally;

[0042] S12, dissolving the silk fibroin prepared in step S11 in 9 mol of LiBr at 60°C for about 4 hours, after the silk fibroin is completely dissolved, dialyze with a dialysis membrane for 72 hours to obtain silk with a mass volume fraction of 6-7w / v% An aqueous protein solution, storing the silk fibroin aqueous solution in a refrigerator at 4°C for use;

[0043] S2, prepare a solution with a concentration of 0.01mol / L hexamethylenetetramethylamine and a concentration of 0.01mol / L zinc nitrate, each take 19ml and mix in equal volumes to obtain 38mL;

[0044] S3, adding the silk fibroi...

Embodiment 2

[0048] S1, dissolving silkworm cocoons after degumming to prepare silk fibroin aqueous solution; specifically:

[0049] S11, cut silkworm cocoons into pieces, weigh 5g of silkworm cocoons, and then boil them in 2L of 0.02mol / L Na2CO3 solution for 30min to remove sericin on the silk surface. After boiling, wash the degummed silk fibroin several times with deionized water and dry it naturally;

[0050]S12, dissolving the silk fibroin prepared in step S11 in 9 mol of LiBr at 60°C for about 4 hours, after the silk fibroin is completely dissolved, dialyze with a dialysis membrane for 72 hours to obtain silk with a mass volume fraction of 6-7w / v% An aqueous protein solution, storing the silk fibroin aqueous solution in a refrigerator at 4°C for use;

[0051] S2, prepare a solution with a concentration of 0.01mol / L hexamethylenetetramethylamine and a concentration of 0.01mol / L zinc nitrate, each take 19ml and mix in equal volumes to obtain 38mL;

[0052] S3, adding the silk fibroin...

Embodiment 3

[0056] S1, dissolving silkworm cocoons after degumming to prepare silk fibroin aqueous solution; specifically:

[0057] S11, cut silkworm cocoons into pieces, weigh 5g of silkworm cocoons, and then boil them in 2L of 0.02mol / L Na2CO3 solution for 30min to remove sericin on the silk surface. After boiling, wash the degummed silk fibroin several times with deionized water and dry it naturally;

[0058] S12, dissolving the silk fibroin prepared in step S11 in 9 mol of LiBr at 60°C for about 4 hours, after the silk fibroin is completely dissolved, dialyze with a dialysis membrane for 72 hours to obtain silk with a mass volume fraction of 6-7w / v% An aqueous protein solution, storing the silk fibroin aqueous solution in a refrigerator at 4°C for use;

[0059] S2, prepare a solution with a concentration of 0.01mol / L hexamethylenetetramethylamine and a concentration of 0.01mol / L zinc nitrate, each take 19ml and mix in equal volumes to obtain 38mL;

[0060] S3, adding the silk fibroi...

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Abstract

The invention relates to the technical field of biomedical materials, and in particular to a zinc oxide nanoparticle preparation method based on silk fibroin bio-template regulation, which includes the following steps: (S1) after being degummed, silkworm cocoons are dissolved, so that aqueous silk fibroin solution is prepared; (S2) zincate solution and alkaline solution are blended, so that mixed solution is obtained; (S3) the aqueous silk fibroin solution obtained step S1 is added into the mixed solution in step S2 to sufficient react; (S4) after solution after the reaction in step S3 is washed and dried, zinc oxide nanoparticles are obtained. Silk fibroin is adopted as a bio-template to regulate the size and morphology of the zinc oxide nanoparticles, the grain size of the obtained zinc oxide nanoparticles is small, the morphology is irregular, the antibacterial property is enhanced, moreover, the operation method is simple, and the cost is low.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to a method for preparing zinc oxide nanoparticles based on silk fibroin biological template regulation. Background technique [0002] The use of antibiotics can significantly improve human resistance to pathogenic bacteria. However, with the abuse of antibiotics, some pathogenic bacteria have developed resistance to antibiotics, which affects the therapeutic effect of antibiotics. Compared with commonly used antibiotics, inorganic antibacterial materials have the advantages of high safety, strong durability, stable antibacterial performance and good heat resistance. Among them, nano-zinc oxide has good biocompatibility, immune regulation ability and antibacterial property, and has the advantages of less dosage, high antibacterial efficiency and low cost in the antibacterial application process. [0003] The antibacterial mechanism of nano-ZnO is mainly as follows: (1)...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C01G9/02B82Y40/00
CPCB82Y40/00C01G9/02C01P2004/03
Inventor 李明吉训明尹志臣闫峰李思颉任长虹蒋芳
Owner XUANWU HOSPITAL OF CAPITAL UNIV OF MEDICAL SCI
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