Quantitative detection method of pneumococcus polysaccharide

Abstract

The invention discloses a quantitative detection method of pneumococcus polysaccharide. According to the method, a combined saccharide prepared from cheap and easily available monosaccharides is used as a standard and replaces a purchased polysaccharide standard, standard curves are built through the combined saccharide and purchased polysaccharide standard, the same batch of pneumococcus polysaccharide samples are detected, the detection results are compared so that a correction coefficient is obtained, in later detection, a standard curve is built through the combined saccharide replacing the purchased standard saccharide, the concentration of the pneumococcus polysaccharide sample is detected, and through correction coefficient correction, the final concentration of the sample to be detected is obtained. The offset between the result and a detection result obtained by purchased standard saccharide as a standard detection sample is less than or equal to 5%. Monosaccharides in the combined saccharide are cheap and easily available, the analysis method is simple, the analysis cost is greatly reduced, the problem of the high cost of the purchased standard saccharide is solved, the experimental result is small and the quantitative detection method is easy to promote. Under appropriate operating conditions, the error is controlled within 0.4%. The quantitative detection method can be used as a quantitative detection method of pneumococcus polysaccharide.

Description

technical field [0001] The invention belongs to the field of drug analysis and relates to a quantitative detection method for pneumococcal polysaccharide. Background technique [0002] Pneumococcus is one of the main pathogenic bacteria that cause invasive meningitis, pneumonia and otitis media in children, and is an important cause of hospitalization and even death of adults and children with pneumonia. Pneumococcus is a Gram-positive bacterium, and its basic virulence factor is Capsular polysaccharides can be divided into different serotypes according to the antigenicity of capsular polysaccharides. So far, 23 serotypes of Streptococcus pneumoniae polysaccharide vaccines have been successfully developed. In the 23-valent pneumococcal polysaccharide vaccine product inspection project, the content of 23-valent pneumococcal polysaccharide is an important detection index of the vaccine. [0003] Existing methods for the quantitative detection of polysaccharides include chromo...

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Problems solved by technology

[0003] Existing methods for the quantitative detection of polysaccharides include chromogenic methods, such as the anthrone-sulfuric acid method (Wu Kai, Dai Zeyou, the improved anthrone method detects the concentration of polysaccharides in the capsular polysaccharide conjugates of Streptococcus pneumoniae (Chinese Journal of Biological Products, 2007, 20 (7): 536-538), but this method simply uses glucose as a standard substance, and obtains the pneumococcal polysaccharide content by establishing a standard curve, so the result often deviates greatly from the true value

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