A compound probiotic agent for improving the immunity of pet dogs and its preparation and application
A technology of compound probiotics and immunity, applied in the direction of biochemical equipment and methods, applications, drug combinations, etc., can solve the problems of reducing the incidence of diarrhea, not providing, etc., to reduce the incidence and degree of incidence, and increase feed intake , the effect of increasing the growth rate
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Embodiment 1
[0053] The preparation of four kinds of probiotics of embodiment 1
[0054] Preparation of Lactobacillus plantarum KT-Lp9 inoculum, Lactobacillus casei zhang inoculum, Lactobacillus plantarum P-8 inoculum, Bifidobacterium animalis subsp. lactis V9 inoculum:
[0055] Separately carry out high-density fermentation of 4 strains of fermentation strains: take one ring of activated Lactobacillus plantarum KT-Lp9, Lactobacillus casei zhang, Lactobacillus plantarum P-8, Bifidobacterium animalis subsp. respectively inoculated into MRS medium, cultured at the same temperature of 33-37°C and at the same speed of 50-100rpm for 18-24 hours to obtain the primary seed liquid respectively; %~10% (v / v) was transferred into the MRS medium again for secondary activation, and the secondary seed solution was obtained after the activation time of 18~24 hours; / v) respectively connected to different fermenter culture medium, the temperature is 33-37°C, the rotation speed is 50-100rpm, the ventilati...
Embodiment 2
[0061] Embodiment 2 Preparation of a compound probiotic agent that improves the immunity of pet dogs
[0062] Compound mixing: the Lactobacillus plantarum KT-Lp9 inoculum prepared by Example 1, Lactobacillus casei zhang inoculum, Lactobacillus plantarum P-8 inoculum, Bifidobacterium animalis milk subsp. V9 inoculum in mass ratio 1: 1:1:2 for mixing, and adding diluted carrier skim milk powder for compounding, and the total number of viable bacteria was 2×10 9 CFU / g, 4×10 9 CFU / g and 1×10 10 CFU / g three specifications of compound probiotics;
[0063] The compound probiotic agent is sub-packaged through a powder packaging machine filled with nitrogen gas, 2g / bag.
Embodiment 3
[0064] Embodiment 3 Preparation of a compound probiotic agent for improving the immunity of pet dogs
[0065] Compound mixing: the Lactobacillus plantarum KT-Lp9 inoculum prepared by Example 1, Lactobacillus casei zhang inoculum, Lactobacillus plantarum P-8 inoculum, Bifidobacterium animalis milk subspecies V9 inoculum in mass ratio 4: 4:4:7 for mixing, and adding diluted carrier skim milk powder for compounding, and the total number of viable bacteria was 2×10 9 CFU / g, 4×10 9 CFU / g and 1×10 10 CFU / g three specifications of compound probiotics;
[0066] The compound probiotic agent is sub-packaged through a powder packaging machine filled with nitrogen gas, 2g / bag.
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