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Ratio-type Ca<2+> fluorescent probe, and synthetic method and application thereof

A fluorescent probe, ratiometric technology, applied in the field of fluorescence imaging and biosensing, can solve the problem of high probe toxicity

Inactive Publication Date: 2017-08-18
EAST CHINA NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently used for Ca 2+ The detected probes are all organic fluorophore-based probes, and most of them are single-excitation and single-emission probes, so the probes are highly toxic and are often affected by factors such as light source or detector drift or complex sample environments. limit

Method used

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  • Ratio-type Ca&lt;2+&gt; fluorescent probe, and synthetic method and application thereof
  • Ratio-type Ca&lt;2+&gt; fluorescent probe, and synthetic method and application thereof
  • Ratio-type Ca&lt;2+&gt; fluorescent probe, and synthetic method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0069] Embodiment 1 ratio type Ca 2+ Preparation of fluorescent probes

[0070] (1)Ca 2+ Ligand Preparation

[0071]NaOH (10 mmol) and o-nitrophenol (10 mmol) were stirred in DMF for 30 minutes. Subsequently, 1,2-dibromoethane (10 mmol) was added and refluxed for 2 h. After the reaction at 160° C., it was cooled to room temperature, diluted with ultrapure water and filtered. The resulting solid was treated with NaHCO 3 The solution was washed several times with water, followed by recrystallization to obtain 1,2-bis(2-nitrophenoxy)ethane.

[0072] The above 1,2-bis(2-nitrophenoxy)ethane (10mmol), carbon powder (10mmol) and FeCl 3 (1mmol) was added to 90% methanol (45g) and heated to reflux, and then 85% hydrazine hydrate (20mmol) was added under reflux to continue the reflux reaction at 100°C for 5h. After the reaction is completed, filter and wash with a small amount of methanol, combine the mother liquor and washing liquid, and obtain a solid after rotary evaporation an...

Embodiment 3

[0084] Embodiment 3 selectivity and anti-interference ability detection

[0085] In order to evaluate the ratio type Ca prepared by Example 1 of the present invention 2+ The selectivity and anti-interference ability of fluorescent probes were investigated separately with the presence of common metal ions, amino acids and common active oxygen 2+ (or H+) coexist, record ratio Ca 2+ Changes in the fluorescent signal of the fluorescent probe. Depend on Figure 7 It can be seen that most substances (except Cu 2+ ) both for Ca 2+ Low detection interference (2+ partially quenched ratiometric Ca 2+ fluorescence of the fluorescent probe, but when Cu 2+ with Ca 2+ Ratio-type Ca 2+ The fluorescence of the fluorescent probe was significantly enhanced, proving that Cu 2+ influence can be ignored. Selectivity and anti-interference experiments illustrate the ratiometric Ca of the present invention 2+ Fluorescent probes have good selectivity and anti-interference ability.

[0086]...

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Abstract

The invention discloses a ratio-type Ca<2+> fluorescent probe and a synthetic method thereof, and belongs to the technical fields of fluorescent imaging and biosensing. The synthetic method comprises the following steps: synthesizing a novel Ca<2+> ligand having two aldehyde functional groups, and then modifying the Ca<2+> ligand, thus obtaining a template molecule; synthesizing fluorescent CuNCs (Copper Nano Clusters) on the basis that the template molecule is prepared, and then coupling with reference fluorescent molecules, thus constructing and obtaining the ratio-type Ca<2+> fluorescent probe in specific response with Ca<2+>. The ratio-type Ca<2+> fluorescent probe has two transmitting signals of the CuNCs and a reference probe, and is capable of correcting interference of environment and eliminating fluctuation of exciting light intensity. The invention also discloses the application of the ratio-type Ca<2+> fluorescent probe in cell imaging and biosensing. The synthetic method disclosed by the invention is simple and convenient, and the ratio-type Ca<2+> fluorescent probe prepared by the invention is high in selectivity and anti-jamming capability.

Description

technical field [0001] The present invention relates to a Ca 2+ A fluorescent probe and its application in cell imaging and biosensing belong to the technical field of fluorescence imaging and biosensing. Background technique [0002] Calcium ion is an indispensable ion for various physiological activities of the body. It is of great significance for maintaining the biopotential on both sides of the cell membrane and maintaining normal nerve conduction function. Normal muscle contraction and relaxation function, nerve-muscle conduction function and the action mechanism of some hormones are all manifested by calcium ions. In addition, intracellular Ca 2+ Has been identified as a key intracellular messenger by which cellular function and gene expression are regulated. Ca 2+ concentration fluctuations. Therefore, it is of great significance to realize the detection of calcium ions. There are currently many methods for Ca 2+ Detection, such as UV-Vis absorption spectrosco...

Claims

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Application Information

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IPC IPC(8): C09K11/58C07C227/18C07C229/18G01N21/64
CPCC09K11/58C07C201/12C07C213/02C07C227/02C07C227/16C07C227/18C07C229/18G01N21/6428G01N2021/6432C07C205/37C07C217/84
Inventor 田阳刘智超王声男敬霞
Owner EAST CHINA NORMAL UNIV
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