Method for preparing nitrate-polluted soil mediating inoculant N4 by virtue of sweet potato starch waste liquid

A sweet potato starch and polluted soil technology, applied in the restoration of polluted soil, chemical instruments and methods, bacteria, etc., can solve the problems of vegetable quality decline, nitrate accumulation, etc.

Inactive Publication Date: 2017-08-11
TIANJIN UNIV OF SCI & TECH
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Problems solved by technology

Vegetables are a kind of crops that are easy to accumulate nitrates. Farmers use excessive nitrogen fertilizers in order to obtain high yields, resulting in the accumulation of nitrates and the decline of vegetable quality.

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  • Method for preparing nitrate-polluted soil mediating inoculant N4 by virtue of sweet potato starch waste liquid
  • Method for preparing nitrate-polluted soil mediating inoculant N4 by virtue of sweet potato starch waste liquid
  • Method for preparing nitrate-polluted soil mediating inoculant N4 by virtue of sweet potato starch waste liquid

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Embodiment Construction

[0022] The present invention will be further described in detail below in conjunction with the accompanying drawings and through specific embodiments. The following embodiments are only descriptive, not restrictive, and cannot limit the protection scope of the present invention.

[0023] A kind of method adopting sweet potato starch waste liquid to prepare nitrate-contaminated soil remediation bacterial agent N4, its transformation step is:

[0024] (1) Raw material waste liquid preparation: The raw material waste liquid is wastewater discharged from a sweet potato starch processing plant, its COD is 20000 mg / L, SS is about 1600 mg / L, pH value is 4-6, and the whole is acidic;

[0025] (2) Strain selection: Strain N4 is a heterotrophic nitrifying bacterium obtained from farmland soil after sampling and acclimation, separation by streaking, screening of strains, and purification by streaking. The specific method is in [Yang Zongzheng, Wang Xin et al. The separation of heterotrop...

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Abstract

The invention relates to a method for preparing a nitrate-polluted soil mediating inoculant N4 by virtue of sweet potato starch waste liquid. On the basis of a bio-transformation method, the sweet potato starch waste liquid, which contains a great amount of nutrient substances such as starch, polysaccharides, pectin, protein and the like, serves as a natural microbiological culture medium to culture beneficial microorganism, namely heterotrophic nitrification bacterium N4; methods for separation, purification and enlarged culture of the N4 bacterium are published in [Separation of Heterotrophic Nitrification Bacteria and Reinforcement of an Activated Sludge Denitrification Effect thereof [J] by YANG Zongzheng, WANG Xin, etc., China Water & Wastewater, 2006, 22 (21): 67-70]; the N4, as the heterotrophic nitrification bacterium, belongs to Gram-negative bacteria; and with the application of the method, a purpose of recycling the waste liquid is achieved, and the waste liquid is applied to bio-remediation of polluted soil, so that circular economy is finally achieved.

Description

technical field [0001] The invention belongs to the field of microbes in biotechnology and the recycling of waste water in environmental engineering, in particular to a method for preparing nitrate polluted soil remediation bacterial agent N4 by using sweet potato starch waste liquid. Background technique [0002] According to reports, 81.2% of the nitrate ingested by the human body comes from vegetables. Therefore, the research on the technology of reducing the accumulation of nitrate in vegetables has become a hot spot that people pay more and more attention to. Vegetables are a kind of crops that are easy to accumulate nitrates. Farmers use excessive nitrogen fertilizers in order to obtain high yields, resulting in the accumulation of nitrates and the decline of vegetable quality. Generally, when the nitrate content in the soil is high, the nitrate content in the vegetables is also high, and vice versa, but there is no one-to-one correspondence between the two. Therefore,...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09C1/10
CPCB09C1/10C02F3/34C12N1/20
Inventor 杨宗政张洁张孟瑶武莉娅曹井国王春虎吴志国田赛华齐赫东傅鹏肖云天冯会芳
Owner TIANJIN UNIV OF SCI & TECH
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